[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus ...[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.展开更多
Ethanol is considered a better fuel than methanol in direct alcohol fuel cells because of the high energy density and low toxicity.Compared with noble metal catalysts,nickel-based catalysts are much cheaper in price.H...Ethanol is considered a better fuel than methanol in direct alcohol fuel cells because of the high energy density and low toxicity.Compared with noble metal catalysts,nickel-based catalysts are much cheaper in price.However,present nickel-based catalysts still surfer from some disadvantages such as low activity and high overpotential.In this paper;we show a new and high efficient nickel-based catalyst for ethanol oxidation.A layer of anodized nickel passivation film(Ni-APF)was formed on the surface of nickel sheet by anodic oxidation method with carbon dots(CDs)as co-catalyst.At the current density of 110 mA·cm^(-2),the potential for Ni-APF/CDs was only 0.541 V(vs.Ag/AgCI),which was 18.8% lower than that of Ni-APF.Low overpotential could reduce electrode thermal loss and increase output energy.Ni-APF/CDs showed 144.4 mA·cm^(-2) peak current density at peak potential 0.662 V(vs.Ag/AgCI),which was 31% higher than that of Ni-APF(110.3 mA·cm^(-2)).In this system,CDs mainly function in the increase of charge-transfer capacity and the promotion oxidation of carbonaceous intermediates.展开更多
Carbon dioxide electrochemical reduction(CO_(2)RR)has been recognized as an efficient way to mitigate CO_(2)emissions and alleviate the pressure on global warming and associated environmental consequences.Gold(Au)is r...Carbon dioxide electrochemical reduction(CO_(2)RR)has been recognized as an efficient way to mitigate CO_(2)emissions and alleviate the pressure on global warming and associated environmental consequences.Gold(Au)is reported as stable and active electrocatalysts to convert CO_(2)to CO at low overpotential due to its moderate adsorption strength of^(*)COOH and^(*)CO.The request for improved catalytic performance,however,is motivated by current unsatisfied catalytic selectivity because of the side hydrogen evolution reaction.In this context,the design of Au based binary catalysts that can boost CO selectivity is of great interest.In the present work,we report that Au nanoparticles can be feasibly dispersed and anchored on silicon nanowires to form Au-Si binary nanomaterials.The Au-Si may stably drive CO_(2)RR with a CO Faraday efficiency of 95.6%at−0.6 V vs.RHE in 0.5 mol/L KHCO_(3)solution.Such selectivity outperforms Au particles by up to 61%.Controlled experiments illustrate that such catalytic enhancement can chiefly be ascribed to electronic effects of binary catalysts.Theoretical calculations reveal that spontaneously produced silicon oxide may not only inhibit hydrogen evolution reaction,but also stabilize the key intermediate^(*)COOH in CO formation.展开更多
基金Supported by Shandong Province Key R&D Program(2021LZGC015)Taishan Industry Leading Talent Project(LJNY201812)。
文摘[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.
基金the National Key Research and Development Program of China(No.2017YFA0204800)the National MCF Energy R&D Program(No.2018YFE0306105)+2 种基金the National Natural Science Foundation of China(No.51902217)the Collaborative Innovation Center of Suzhou Nano Science&Technology,the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the 111 Project.
文摘Ethanol is considered a better fuel than methanol in direct alcohol fuel cells because of the high energy density and low toxicity.Compared with noble metal catalysts,nickel-based catalysts are much cheaper in price.However,present nickel-based catalysts still surfer from some disadvantages such as low activity and high overpotential.In this paper;we show a new and high efficient nickel-based catalyst for ethanol oxidation.A layer of anodized nickel passivation film(Ni-APF)was formed on the surface of nickel sheet by anodic oxidation method with carbon dots(CDs)as co-catalyst.At the current density of 110 mA·cm^(-2),the potential for Ni-APF/CDs was only 0.541 V(vs.Ag/AgCI),which was 18.8% lower than that of Ni-APF.Low overpotential could reduce electrode thermal loss and increase output energy.Ni-APF/CDs showed 144.4 mA·cm^(-2) peak current density at peak potential 0.662 V(vs.Ag/AgCI),which was 31% higher than that of Ni-APF(110.3 mA·cm^(-2)).In this system,CDs mainly function in the increase of charge-transfer capacity and the promotion oxidation of carbonaceous intermediates.
基金supported by the National Key Research and Development Program of China (No. 2020YFA0406103)National Natural Science Foundation of China (Nos. 51902217 and 21771134)+4 种基金National Key Research and Development Program of China(No. 2017YFA0204800)National MCF Energy R&D Program (No. 2018YFE0306105)the Suzhou Key Laboratory of Functional Nano & Soft Materials, Collaborative Innovation Center of Suzhou Nano Science & Technologythe 111 ProjectJoint International Research Laboratory of Carbon-Based Functional Materials and Devices
文摘Carbon dioxide electrochemical reduction(CO_(2)RR)has been recognized as an efficient way to mitigate CO_(2)emissions and alleviate the pressure on global warming and associated environmental consequences.Gold(Au)is reported as stable and active electrocatalysts to convert CO_(2)to CO at low overpotential due to its moderate adsorption strength of^(*)COOH and^(*)CO.The request for improved catalytic performance,however,is motivated by current unsatisfied catalytic selectivity because of the side hydrogen evolution reaction.In this context,the design of Au based binary catalysts that can boost CO selectivity is of great interest.In the present work,we report that Au nanoparticles can be feasibly dispersed and anchored on silicon nanowires to form Au-Si binary nanomaterials.The Au-Si may stably drive CO_(2)RR with a CO Faraday efficiency of 95.6%at−0.6 V vs.RHE in 0.5 mol/L KHCO_(3)solution.Such selectivity outperforms Au particles by up to 61%.Controlled experiments illustrate that such catalytic enhancement can chiefly be ascribed to electronic effects of binary catalysts.Theoretical calculations reveal that spontaneously produced silicon oxide may not only inhibit hydrogen evolution reaction,but also stabilize the key intermediate^(*)COOH in CO formation.