The effect of NaCI and proline on somatic embryos formation and germination of date palm （Phoenix dactylifera L.） cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with d...The effect of NaCI and proline on somatic embryos formation and germination of date palm （Phoenix dactylifera L.） cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with different concentrations of salt （NaCI） （0.5%, 1%, 1.5%, 2%, 2.5% and 3%, respectively） and proline （25, 50 and 75 mg L1） either individually or in combination. Results showed that the addition of NaC1 （starting at 1%） caused a significant increase in the cylindrical embryos formation time, while their lengths and fresh weights were decreased at salt concentrations （0.5% and 2%）. No significant effect was noticed on the formation time by using proline except at concentration of 25 mg L^-1 which caused an increase in their lengths and fresh weights. The interaction between all salt concentrations and proline （25 mg LL） caused a significant increase in the formation time as well as in their lengths and fresh weights. The addition of NaC1 to the media caused a significant effect on the germination time and a reduction on the percentages of somatic embryo germination starting at 2% and the following ones. A reduction in the lengths of radicals and the plumules of the formed embryos was noticed at 1.5% NaCI. Proline addition reduced the initial time and increased the germination percentages as well as the lengths of radicals and plumules. The interaction between salt and proline treatments caused a significant reduction in the initiation time and an increase in the germination percentages and lengths of radicals and plumules.展开更多
文摘The effect of NaCI and proline on somatic embryos formation and germination of date palm （Phoenix dactylifera L.） cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with different concentrations of salt （NaCI） （0.5%, 1%, 1.5%, 2%, 2.5% and 3%, respectively） and proline （25, 50 and 75 mg L1） either individually or in combination. Results showed that the addition of NaC1 （starting at 1%） caused a significant increase in the cylindrical embryos formation time, while their lengths and fresh weights were decreased at salt concentrations （0.5% and 2%）. No significant effect was noticed on the formation time by using proline except at concentration of 25 mg L^-1 which caused an increase in their lengths and fresh weights. The interaction between all salt concentrations and proline （25 mg LL） caused a significant increase in the formation time as well as in their lengths and fresh weights. The addition of NaC1 to the media caused a significant effect on the germination time and a reduction on the percentages of somatic embryo germination starting at 2% and the following ones. A reduction in the lengths of radicals and the plumules of the formed embryos was noticed at 1.5% NaCI. Proline addition reduced the initial time and increased the germination percentages as well as the lengths of radicals and plumules. The interaction between salt and proline treatments caused a significant reduction in the initiation time and an increase in the germination percentages and lengths of radicals and plumules.
