Supercritical fluid chromatography(SFC),which uses supercritical fluid with higher diffusivity and lower viscosity than liquid as its mobile phase,can provide fast,green,high-throughput and good performance methods fo...Supercritical fluid chromatography(SFC),which uses supercritical fluid with higher diffusivity and lower viscosity than liquid as its mobile phase,can provide fast,green,high-throughput and good performance methods for the analysis of complicated samples.It is an advantageous alternative for the separation of polar and nonpolar compounds,and even chiral isomers.As an important constituent of living organisms,lipids and the related lipidomics researches have attracted dramatic attention during the past years.The separation and identification of lipids and lipid isomers are of great significance and remain challenging.In this mini-review,the principle of SFC and its applications in lipid isomer separation are summarized.展开更多
Ambient mass spectrometry facilitates surface detection under ambient conditions and has quickly found its applications in various areas.However,the sensitivity of AMS methods is usually not as good as that of encapsu...Ambient mass spectrometry facilitates surface detection under ambient conditions and has quickly found its applications in various areas.However,the sensitivity of AMS methods is usually not as good as that of encapsulated ion sources due to less efficient ion transmission.In this work,a briefly machined external ion funnel(threepole electrostatic lens)was adopted and installed between direct analysis in real time and the mass spectrometer inlet to increase detection sensitivity.Software investigation with SIMION version 8.0 was employed to calculate electric fields and to simulate the trajectories of charged particles in the electric field.Both the geometrical parameters of the ion funnel and the electric parameters were studied to obtain the optimal ion focusing results,which demonstrated the ion funnel equipment would greatly improve the ion transmission efficiency.The equipment showed good prospect in direct analysis in real time-mass spectrometry analysis in the future.展开更多
A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis,which was then applied to separate and identify seven classes of sphingolipids in plasma.The time-consuming of this novel method was mu...A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis,which was then applied to separate and identify seven classes of sphingolipids in plasma.The time-consuming of this novel method was much shorter than previous 2D LC–MS method,while the sensitivity,repeatability and resolution for sphingolipids analysis were better or comparable.Besides,the differences of lipids in human plasma among diabetes mellitus patients,diabetes mellitus associated with atherosclerosis patients and control subjects were also compared.Finally,12 sphingolipids species were identified as the amounts of which in diabetes mellitus associated with atherosclerosis patients were significantly higher than them in diabetes mellitus patients(VIP>1.0,p value<0.05,fold change>1.5).As the isomers of GalCs and GluCs were successfully separated,it was found that five GluCs,rather than GalCs,were included in these 12 potential biomarkers.These results suggest the need to separate GalCs from GluCs,and also indicated that there may be some extra activation pathways of diabetes mellitus associated with atherosclerosis,different from the atherosclerosis pathway and the diabetes mellitus pathway,causing cardiovascular disease.展开更多
Hepatocellular carcinoma(HCC)accounts for the major primary liver cancer,and its morbidity and mortality rates are very high.Identification of novel biomarkers for the early diagnosis and treatment of this disease is ...Hepatocellular carcinoma(HCC)accounts for the major primary liver cancer,and its morbidity and mortality rates are very high.Identification of novel biomarkers for the early diagnosis and treatment of this disease is of crucial importance.In this study,a continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography—quadrupole time-of-flight mass spectrometry(NP/RP 2D LC-QToF/MS)method was used to detect lipid species in human plasma from HCC patients and healthy controls.As a result,the two groups were clearly separated by principal component analysis.Besides,23 lipid species with corrected p value<0.05 and fold change>1.5 were proposed as potential lipid biomarkers for HCC.Among them,9 lipid species,including galactosylceramide(36:5),free fatty acid(20:4),phosphatidylethanolamine(40:6),phosphatidylethanolamine(38:6),diacylglycerol(40:5),diacylglycerol(44:2),lactosylceramide(40:3),phosphatidylcholine(40:6),and free fatty acid(22:5),exhibited excellent ability to distinguish HCC patients from healthy controls with the area under the receiver-operating characteristic curve>0.900,showing promising clinical value.Taken together,these results indicate the great potential of NP/RP 2D LC-QToF/MS method in disease diagnosis,which might be helpful in clinical practice.展开更多
Metabolomics has become a valuable approach to discover novel biomarkers and to increase the mechanistic understanding of many deceases,while lipidomics is one of the hottest points of research areas related to metabo...Metabolomics has become a valuable approach to discover novel biomarkers and to increase the mechanistic understanding of many deceases,while lipidomics is one of the hottest points of research areas related to metabolomics,which attracted dramatic attention during the past years.Therefore,this special issue focuses on the analytical methods for metabolomics and lipidomics to provide our readers with recent developments in these areas.展开更多
基金the National Natural Science Foundation of China(Nos.21527809 and 21175005).
文摘Supercritical fluid chromatography(SFC),which uses supercritical fluid with higher diffusivity and lower viscosity than liquid as its mobile phase,can provide fast,green,high-throughput and good performance methods for the analysis of complicated samples.It is an advantageous alternative for the separation of polar and nonpolar compounds,and even chiral isomers.As an important constituent of living organisms,lipids and the related lipidomics researches have attracted dramatic attention during the past years.The separation and identification of lipids and lipid isomers are of great significance and remain challenging.In this mini-review,the principle of SFC and its applications in lipid isomer separation are summarized.
基金This work was financially supported by the National Natural Science Foundation of China(Grant Nos.21275012 and 21175008).
文摘Ambient mass spectrometry facilitates surface detection under ambient conditions and has quickly found its applications in various areas.However,the sensitivity of AMS methods is usually not as good as that of encapsulated ion sources due to less efficient ion transmission.In this work,a briefly machined external ion funnel(threepole electrostatic lens)was adopted and installed between direct analysis in real time and the mass spectrometer inlet to increase detection sensitivity.Software investigation with SIMION version 8.0 was employed to calculate electric fields and to simulate the trajectories of charged particles in the electric field.Both the geometrical parameters of the ion funnel and the electric parameters were studied to obtain the optimal ion focusing results,which demonstrated the ion funnel equipment would greatly improve the ion transmission efficiency.The equipment showed good prospect in direct analysis in real time-mass spectrometry analysis in the future.
基金the National Key R&D Program of China(2017YFC0906800)National Natural Science Foundation of China(Grant nos.21527809 and 21175005).
文摘A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis,which was then applied to separate and identify seven classes of sphingolipids in plasma.The time-consuming of this novel method was much shorter than previous 2D LC–MS method,while the sensitivity,repeatability and resolution for sphingolipids analysis were better or comparable.Besides,the differences of lipids in human plasma among diabetes mellitus patients,diabetes mellitus associated with atherosclerosis patients and control subjects were also compared.Finally,12 sphingolipids species were identified as the amounts of which in diabetes mellitus associated with atherosclerosis patients were significantly higher than them in diabetes mellitus patients(VIP>1.0,p value<0.05,fold change>1.5).As the isomers of GalCs and GluCs were successfully separated,it was found that five GluCs,rather than GalCs,were included in these 12 potential biomarkers.These results suggest the need to separate GalCs from GluCs,and also indicated that there may be some extra activation pathways of diabetes mellitus associated with atherosclerosis,different from the atherosclerosis pathway and the diabetes mellitus pathway,causing cardiovascular disease.
基金the National Natural Science Foundation of China(Grant numbers,21527809 and 21175005).
文摘Hepatocellular carcinoma(HCC)accounts for the major primary liver cancer,and its morbidity and mortality rates are very high.Identification of novel biomarkers for the early diagnosis and treatment of this disease is of crucial importance.In this study,a continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography—quadrupole time-of-flight mass spectrometry(NP/RP 2D LC-QToF/MS)method was used to detect lipid species in human plasma from HCC patients and healthy controls.As a result,the two groups were clearly separated by principal component analysis.Besides,23 lipid species with corrected p value<0.05 and fold change>1.5 were proposed as potential lipid biomarkers for HCC.Among them,9 lipid species,including galactosylceramide(36:5),free fatty acid(20:4),phosphatidylethanolamine(40:6),phosphatidylethanolamine(38:6),diacylglycerol(40:5),diacylglycerol(44:2),lactosylceramide(40:3),phosphatidylcholine(40:6),and free fatty acid(22:5),exhibited excellent ability to distinguish HCC patients from healthy controls with the area under the receiver-operating characteristic curve>0.900,showing promising clinical value.Taken together,these results indicate the great potential of NP/RP 2D LC-QToF/MS method in disease diagnosis,which might be helpful in clinical practice.
文摘Metabolomics has become a valuable approach to discover novel biomarkers and to increase the mechanistic understanding of many deceases,while lipidomics is one of the hottest points of research areas related to metabolomics,which attracted dramatic attention during the past years.Therefore,this special issue focuses on the analytical methods for metabolomics and lipidomics to provide our readers with recent developments in these areas.
