Orally absorbable gold nanoparticles(AuNP)having cancer ablation therapy is strongly demanded to treat glioblastoma multiforme(GBM)for patients with its highest incidence rate.Here,we develop a milk protein lactoferri...Orally absorbable gold nanoparticles(AuNP)having cancer ablation therapy is strongly demanded to treat glioblastoma multiforme(GBM)for patients with its highest incidence rate.Here,we develop a milk protein lactoferrin-conjugated AuNP for its oral absorption and targeting to the GBM through the interaction between lactoferrin(Lf)and lactoferrin receptor(LfR)that is highly expressed in the intestine,blood-brain barrier and GBM.For stability and long circulation of AuNP,glutathione and polyethylene glycol(PEG)is introduced,which is called to Lf-PEG-AuNP.When Lf-PEG-AuNP are orally administered to orthotopic GBM-bearing mice,11-fold and 8-fold higher concentrations of AuNP are measured in bloodstreams and GBM in the brain,respectively,compared with unconjugated-AuNP.Therefore,orally administered Lf-PEG-AuNP exhibit an outstanding temperature rise in GBM by irradiating laser and significantly reduce tumor volume.Collectively,we suggest that the Lf-PEG-AuNP can fundamentally target GBM in the brain through oral absorption,and that its efficient photothermal therapy is possible.展开更多
基金supported by the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT&Future Planning(NRF-2020R1A2C3005834).
文摘Orally absorbable gold nanoparticles(AuNP)having cancer ablation therapy is strongly demanded to treat glioblastoma multiforme(GBM)for patients with its highest incidence rate.Here,we develop a milk protein lactoferrin-conjugated AuNP for its oral absorption and targeting to the GBM through the interaction between lactoferrin(Lf)and lactoferrin receptor(LfR)that is highly expressed in the intestine,blood-brain barrier and GBM.For stability and long circulation of AuNP,glutathione and polyethylene glycol(PEG)is introduced,which is called to Lf-PEG-AuNP.When Lf-PEG-AuNP are orally administered to orthotopic GBM-bearing mice,11-fold and 8-fold higher concentrations of AuNP are measured in bloodstreams and GBM in the brain,respectively,compared with unconjugated-AuNP.Therefore,orally administered Lf-PEG-AuNP exhibit an outstanding temperature rise in GBM by irradiating laser and significantly reduce tumor volume.Collectively,we suggest that the Lf-PEG-AuNP can fundamentally target GBM in the brain through oral absorption,and that its efficient photothermal therapy is possible.
