Aberrant cytological localization of p16 and CDK4 in colorectal epithelia in the normal adenoma carcinoma sequence

AIM: To study the correlation between the patterns of subcellular expression of p16 and CDK4 in colorectal epithelia in the normal-adenoma-carcinoma sequence. METHODS: Paraffin sections of 43 cases of normal colorectal epithelia and corresponding adenomas as well as carcinomas were analysed immunocytochemically for subcellular expression of p16 and CDK4 proteins. RESULTS: Most carcinomas showed more cytoplasmic overexpression for p16 and CDK4 than the adenomas from which they arised or the adjacent normal mucosa. Most normal or non-neoplastic epithelia showed more p16 and CDK4 expression in the nucleus than their adjacent adenomas and carcinomas. There was a significant difference between the subcellular expression pattern of p16 and CDK4 in normal-adenoma-carcinoma sequence epithelia (P < 0.001). Neither p16 nor CDK4 subcellular patterns correlated with histological grade or Dukes’ stage. CONCLUSION: Interaction of expression of p16 and CDK4 plays an important role in the Rb/p16 pathway. Overexpression of p16 and CDK4 in the cytoplasm, as well as loss expression of p16 in the nucleus might be important in the evolution of colorectal carcinoma from adenoma and, of adenoma from normal epithelia.

Clinico-pathological aspects of colorectal serrated adenomas

瞄准：学习颜色的协会有侵略的癌，本地复发， synchronicity 和损害的元慢性的表面的锯齿状腺瘤(SA ) 。方法：从在一个八年的时期(1987-1995 ) 上的 1,096 个病人的 4,536 息肉的一个总数回顾地被检验。显示出至少 50% serrated 建筑学的腺瘤被三称为 SA 考察病理学家。结果：(2%) 91 所有息肉被称为 SA，它在 46 个病人被发现。侵略的癌从 46 在 3 被看见(6.4%) 病人，一个人是家庭腺瘤息肉病(FAP ) 的一个盒子。男优势被注意，发育异常的温和的度的特征在多数被看见(n=75， 83%) 锯齿状腺瘤。后续变化了有 5.75 年的一吝啬的时间的 1-12 年。SA 的复发在 3 被看见(6.4%) 盒子，在 16 的同步 SA (34.8%) 在 9 的盒子和异时 SA (19.6%) 盒子。结论：侵略的癌在锯齿状腺瘤产生是稀罕的，为 2 的财务(4.3%) 在这个系列的 cases studied。