Soil microbial biomass carbon (MBC), β-glucosidase, acid phosphatase and fluorescein diacetate (FDA) activities and bacterial community structure were assessed in a long-term (26 years) experiment, at physiological s...Soil microbial biomass carbon (MBC), β-glucosidase, acid phosphatase and fluorescein diacetate (FDA) activities and bacterial community structure were assessed in a long-term (26 years) experiment, at physiological stages of sorghum growth, comparing different management methods for organic (manure, straw residues) and inorganic (urea) amendments at the INERA field station in Saria (Burkina Faso). Annual application of manure led to the highest soil microbial biomass and enzyme activities. Investigations indicated that only microbial biomass and β-glucosidase activities were affected during the cropping season. Phosphatase and FDA enzyme activities did not depend on the crop development stages. The application of N fertilizer modified phosphatase and FDA enzyme activities, the activities being higher in soils amended with N fertilizer. The bacterial community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the eubacterial 16S rRNA gene. Cluster analysis of PCR-DGGE patterns showed two major clusters, the first containing the mineral fertilization and straw treatments and the second, the straw + urea, manure and manure + urea treatments. Sorghum grain yields were the highest for manure treatments. In this long-term experiment, applying straw did not produce a better grain yield than that obtained in the un-amended plot.展开更多
The aim was to study the effects of organic management like the application of organic matters on crop production. This research is placed in the context of climate change impact mitigation. A field experiment was con...The aim was to study the effects of organic management like the application of organic matters on crop production. This research is placed in the context of climate change impact mitigation. A field experiment was conducted during the dry season. Rainfall inputs were simulated by irrigation to study the effects of water stress during the flowering period of a grain on the agronomic and the physiological behavior of the plant. The measurements were made on the volumetric soil moisture, stomatal conductance, and leaf area index (LAI), grain yield, straw and weight of 100 grains. The water use efficiency (WUE) and yield losses were evaluated. The results of the volumetric soil moisture showed that the use of localized input under water stress (STR-T1) recorded the lowest moisture in the surface horizons. Treatment with localized input under water stress with or without fertilization (STR-T1, STR-T1 + N) showed an ability of stomatal regulation compared to the control (STR- T0) and the input application by spreading (STR- T2). (STR-T1 + N) has initiated an early stomatal closure of the plant because of the effect of nitrogen. However, despite a more pronounced water stress with stomatal closure, the LAI and the grain yield were greater with (STR-T1) and (STR-T1 + N). The results showed that the inputs of localized organic fertilization with or without nitrogen grain yields were the highest regardless of the hydric regime applied. However the losses of grain yield were higher in treatments with organic inputs in spreading and localized under water stress. The WUE by the crop was reduced compared to the control with organic inputs under STR. In this study we show that the use of organic matter increases de farmers risk and this notion of risk is high and it is necessary to consider this risk in the proposals of technical innovations.展开更多
Saba senegalensis is a wild edible fruit plant species with a high economic potential which can be used to fight food insecurity in rural areas and to reduce poverty. Domestication programs are being carried out to bo...Saba senegalensis is a wild edible fruit plant species with a high economic potential which can be used to fight food insecurity in rural areas and to reduce poverty. Domestication programs are being carried out to boost production. However, no studies have been done to determine the optimal soil properties for growing S. senegalensis. This study was carried out to determine the effects of the physical and chemical properties of different substrates on the mycorrhization and growth of S. senegalensis under semi-controlled conditions. S. senegalensis seeds were grown for 4 months in the nursery using five substrates: S1 (1/2 sand + 1/2 potting soil), S2 (1/3 sand + 2/3 potting soil), S3 (2/3 sand + 1/3 potting soil), S4 (potting soil) and S5 (sand). The intensity of mycorrhization was highest for plants grown on substrates with a lowest clay, silt, and nutrient content S3 (29.5%) and S5 (23.5%) respectively. Substrates with much higher clay and silt content stimulated better the growth of S. senegalensis than substrates with lower silt clay and nutrient content. In the context of domestication, the quality of the substrates could be used to stimulate the mycorrhization and the growth of S. senegalensis and thus quickly produce vigorous plants.展开更多
文摘Soil microbial biomass carbon (MBC), β-glucosidase, acid phosphatase and fluorescein diacetate (FDA) activities and bacterial community structure were assessed in a long-term (26 years) experiment, at physiological stages of sorghum growth, comparing different management methods for organic (manure, straw residues) and inorganic (urea) amendments at the INERA field station in Saria (Burkina Faso). Annual application of manure led to the highest soil microbial biomass and enzyme activities. Investigations indicated that only microbial biomass and β-glucosidase activities were affected during the cropping season. Phosphatase and FDA enzyme activities did not depend on the crop development stages. The application of N fertilizer modified phosphatase and FDA enzyme activities, the activities being higher in soils amended with N fertilizer. The bacterial community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the eubacterial 16S rRNA gene. Cluster analysis of PCR-DGGE patterns showed two major clusters, the first containing the mineral fertilization and straw treatments and the second, the straw + urea, manure and manure + urea treatments. Sorghum grain yields were the highest for manure treatments. In this long-term experiment, applying straw did not produce a better grain yield than that obtained in the un-amended plot.
文摘The aim was to study the effects of organic management like the application of organic matters on crop production. This research is placed in the context of climate change impact mitigation. A field experiment was conducted during the dry season. Rainfall inputs were simulated by irrigation to study the effects of water stress during the flowering period of a grain on the agronomic and the physiological behavior of the plant. The measurements were made on the volumetric soil moisture, stomatal conductance, and leaf area index (LAI), grain yield, straw and weight of 100 grains. The water use efficiency (WUE) and yield losses were evaluated. The results of the volumetric soil moisture showed that the use of localized input under water stress (STR-T1) recorded the lowest moisture in the surface horizons. Treatment with localized input under water stress with or without fertilization (STR-T1, STR-T1 + N) showed an ability of stomatal regulation compared to the control (STR- T0) and the input application by spreading (STR- T2). (STR-T1 + N) has initiated an early stomatal closure of the plant because of the effect of nitrogen. However, despite a more pronounced water stress with stomatal closure, the LAI and the grain yield were greater with (STR-T1) and (STR-T1 + N). The results showed that the inputs of localized organic fertilization with or without nitrogen grain yields were the highest regardless of the hydric regime applied. However the losses of grain yield were higher in treatments with organic inputs in spreading and localized under water stress. The WUE by the crop was reduced compared to the control with organic inputs under STR. In this study we show that the use of organic matter increases de farmers risk and this notion of risk is high and it is necessary to consider this risk in the proposals of technical innovations.
文摘Saba senegalensis is a wild edible fruit plant species with a high economic potential which can be used to fight food insecurity in rural areas and to reduce poverty. Domestication programs are being carried out to boost production. However, no studies have been done to determine the optimal soil properties for growing S. senegalensis. This study was carried out to determine the effects of the physical and chemical properties of different substrates on the mycorrhization and growth of S. senegalensis under semi-controlled conditions. S. senegalensis seeds were grown for 4 months in the nursery using five substrates: S1 (1/2 sand + 1/2 potting soil), S2 (1/3 sand + 2/3 potting soil), S3 (2/3 sand + 1/3 potting soil), S4 (potting soil) and S5 (sand). The intensity of mycorrhization was highest for plants grown on substrates with a lowest clay, silt, and nutrient content S3 (29.5%) and S5 (23.5%) respectively. Substrates with much higher clay and silt content stimulated better the growth of S. senegalensis than substrates with lower silt clay and nutrient content. In the context of domestication, the quality of the substrates could be used to stimulate the mycorrhization and the growth of S. senegalensis and thus quickly produce vigorous plants.
