The outbreak of oxidative stress, inflammatory diseases and mycoses, constitute an important public health problem. This could be due to the increase of risk factors, side effects and expensive therapeutic molecules a...The outbreak of oxidative stress, inflammatory diseases and mycoses, constitute an important public health problem. This could be due to the increase of risk factors, side effects and expensive therapeutic molecules available. In the aim to find the potential spring of new therapeutic molecules with efficient and wide spectrum of action, the antiradical, anti-inflammatory and antifungal activities of two essential oils were evaluated. The essential oils were extracted by hydrodistillation. The antiradical activity was evaluated to use DPPH （2,2-diphenyl-l-picrylhydrazyl） scavenging method and the anti-inflammatory activity was determined using the enzymatic method. The disc diffusion and dilution methods were used to evaluate growth inhibition of three yeasts, three moulds and three dermatophytes. The yields of extraction of Apium graveolens and Thymus vulgaris essential oils were 0.14% and 0.32% respectively. These essential oils showed antiradical properties with respective SC50 of 0.41 and 0.06 g/L for Apium graveolens and Thymus vulgaris. Only Thymus vulgaris presented an anti-inflammatory activity with an IC50 of 0.19 g/L. Cryptococcus neoformans was the most susceptible fungal strain while C. albicans was the most resistant one. The results were compared with the standard antifungal. These results show that these essential oils could be exploited as potential spring of molecules endowed with antiradical, anti-inflammatory and antifungal activities.展开更多
Due to the harmful effect of free radical on physiological and pathological state of our body on one hand, and the increase of the fungal infection on the other hand, drug that can reduce free radical and inhibit fung...Due to the harmful effect of free radical on physiological and pathological state of our body on one hand, and the increase of the fungal infection on the other hand, drug that can reduce free radical and inhibit fungal growth is needed. This work aims to evaluate the antioxidant and antifungal activities of the Ocimum gratissimum L. essential oils from center and west region of Cameroon. Essential oil was analyzed by GC （gas chromatography） and GC coupled with MS （mass spectrometry）. The antioxidant activities of essential oils were studied by the Diphenylpicrylhydrazyl method and the β-carotene bleaching test. The antifungal activities were assessed using micro-dilution technique for yeasts and agar dilution method for Aspergillus. Thymol and γ-terpinene, eugenol and thymol were the major compounds for Yaounde and Dschang, respectively. The scavenging capacity of sample from Dschang was higher than that of Yaounde. Also, the β-carotene bleaching tests of the sample from Dschang were better than that from Yaounde. The antifungal activity of the sample from Yaounde was higher than that from Dschang on yeasts and Aspergillus isolates, respectively. This work presents and compares the chemical composition, antioxidant and antifungal activity of Ocimum gratissimum essential oil from center and west region of Cameroon.展开更多
文摘The outbreak of oxidative stress, inflammatory diseases and mycoses, constitute an important public health problem. This could be due to the increase of risk factors, side effects and expensive therapeutic molecules available. In the aim to find the potential spring of new therapeutic molecules with efficient and wide spectrum of action, the antiradical, anti-inflammatory and antifungal activities of two essential oils were evaluated. The essential oils were extracted by hydrodistillation. The antiradical activity was evaluated to use DPPH （2,2-diphenyl-l-picrylhydrazyl） scavenging method and the anti-inflammatory activity was determined using the enzymatic method. The disc diffusion and dilution methods were used to evaluate growth inhibition of three yeasts, three moulds and three dermatophytes. The yields of extraction of Apium graveolens and Thymus vulgaris essential oils were 0.14% and 0.32% respectively. These essential oils showed antiradical properties with respective SC50 of 0.41 and 0.06 g/L for Apium graveolens and Thymus vulgaris. Only Thymus vulgaris presented an anti-inflammatory activity with an IC50 of 0.19 g/L. Cryptococcus neoformans was the most susceptible fungal strain while C. albicans was the most resistant one. The results were compared with the standard antifungal. These results show that these essential oils could be exploited as potential spring of molecules endowed with antiradical, anti-inflammatory and antifungal activities.
文摘Due to the harmful effect of free radical on physiological and pathological state of our body on one hand, and the increase of the fungal infection on the other hand, drug that can reduce free radical and inhibit fungal growth is needed. This work aims to evaluate the antioxidant and antifungal activities of the Ocimum gratissimum L. essential oils from center and west region of Cameroon. Essential oil was analyzed by GC （gas chromatography） and GC coupled with MS （mass spectrometry）. The antioxidant activities of essential oils were studied by the Diphenylpicrylhydrazyl method and the β-carotene bleaching test. The antifungal activities were assessed using micro-dilution technique for yeasts and agar dilution method for Aspergillus. Thymol and γ-terpinene, eugenol and thymol were the major compounds for Yaounde and Dschang, respectively. The scavenging capacity of sample from Dschang was higher than that of Yaounde. Also, the β-carotene bleaching tests of the sample from Dschang were better than that from Yaounde. The antifungal activity of the sample from Yaounde was higher than that from Dschang on yeasts and Aspergillus isolates, respectively. This work presents and compares the chemical composition, antioxidant and antifungal activity of Ocimum gratissimum essential oil from center and west region of Cameroon.
