Determining the species of origin of skeletal remains is critical in a forensic and anthropologic context. However, there are very few methods that use a chemical approach to assist in this determination. In this stud...Determining the species of origin of skeletal remains is critical in a forensic and anthropologic context. However, there are very few methods that use a chemical approach to assist in this determination. In this study, Raman spectroscopy was used to discriminate bone samples originating from four different species (bovine, porcine, turkey and chicken). Spectra were obtained using a near infrared laser at 785-nm. All spectra were combined in a single matrix and processed using partial least squares discriminate analysis (PLS-DA) with leave-one-out cross-validation. Three com-ponents were found to adequately describe the system. The first two components which contributed over 85% of spec-tral data was seen to completely separate the four species of origin in a two dimensional scores plot. A 95% confidence interval was draw around score points of each species class with very slight overlap. The first two components were seen to have large contributions from bioapatite and collagen, the main components of bone. This study serves as a preliminary investigation to evaluate the effectiveness of Raman spectroscopy to discriminate the species of origin of bone tissue.展开更多
Two critical issues in forensic science are identifying body fluid traces found at crime scenes and preserving them for DNA analysis. However, the majority of current biochemical tests for body fluid identification, w...Two critical issues in forensic science are identifying body fluid traces found at crime scenes and preserving them for DNA analysis. However, the majority of current biochemical tests for body fluid identification, which are applicable at the crime scene, are presumptive and destructive to the sample. Raman Spectroscopy provides a suitable alternative to current methods as a nondestructive, confirmatory, and potentially in field method. Our laboratory has developed a chemometric model for the identification of five main body fluids using Raman spectroscopy. This model was developed using samples obtained from healthy donors. Thus, it is of most importance for the forensic application of the method to validate its performance for donors with diseases that might affect the biochemical composition of body fluids. In this study, the developed method was validated using peripheral blood samples acquired from donors with Celiac Disease, Sickle Cell Anemia, and Type 2 Diabetes. It was shown that the method correctly identified all samples as peripheral blood indicating that no false positives could occur because the blood traces were originated from donors suffering from the diseases.展开更多
文摘Determining the species of origin of skeletal remains is critical in a forensic and anthropologic context. However, there are very few methods that use a chemical approach to assist in this determination. In this study, Raman spectroscopy was used to discriminate bone samples originating from four different species (bovine, porcine, turkey and chicken). Spectra were obtained using a near infrared laser at 785-nm. All spectra were combined in a single matrix and processed using partial least squares discriminate analysis (PLS-DA) with leave-one-out cross-validation. Three com-ponents were found to adequately describe the system. The first two components which contributed over 85% of spec-tral data was seen to completely separate the four species of origin in a two dimensional scores plot. A 95% confidence interval was draw around score points of each species class with very slight overlap. The first two components were seen to have large contributions from bioapatite and collagen, the main components of bone. This study serves as a preliminary investigation to evaluate the effectiveness of Raman spectroscopy to discriminate the species of origin of bone tissue.
文摘Two critical issues in forensic science are identifying body fluid traces found at crime scenes and preserving them for DNA analysis. However, the majority of current biochemical tests for body fluid identification, which are applicable at the crime scene, are presumptive and destructive to the sample. Raman Spectroscopy provides a suitable alternative to current methods as a nondestructive, confirmatory, and potentially in field method. Our laboratory has developed a chemometric model for the identification of five main body fluids using Raman spectroscopy. This model was developed using samples obtained from healthy donors. Thus, it is of most importance for the forensic application of the method to validate its performance for donors with diseases that might affect the biochemical composition of body fluids. In this study, the developed method was validated using peripheral blood samples acquired from donors with Celiac Disease, Sickle Cell Anemia, and Type 2 Diabetes. It was shown that the method correctly identified all samples as peripheral blood indicating that no false positives could occur because the blood traces were originated from donors suffering from the diseases.
