AIM To determine if sorafenib, an antineoplastic agent, could prevent the development of spinal epidural fibrosis(EF). METHODS The study used CD105 and osteopontin antibodiesin an immunohistochemical approach to quant...AIM To determine if sorafenib, an antineoplastic agent, could prevent the development of spinal epidural fibrosis(EF). METHODS The study used CD105 and osteopontin antibodiesin an immunohistochemical approach to quantify EF that occurred as a consequence of laminectomy in rats. Wistar albino rats(n = 16) were divided into two groups: control(L1-2 level laminectomy only) and sorafenib treatment(L1-2 level laminectomy + topical sorafenib). The animals were euthanatized after 6 wk, and the EF tissues were examined for histopathological changes after immunohistochemical staining. The EF grades were assigned to the tissues, and the treatment and control groups were compared. RESULTS The EF thickness, inflammatory cell density, and arachnoid adherences determined by light microscopy were significantly higher in the control group compared to the sorafenib-treated group. Based on fibrosis scores, the extent of EF in the treatment group was significantly lower than in the controls. Immunohistochemical staining for CD105 to identify microvessels revealed that the EF grades based on vessel count were significantly lower in the treatment group. Staining for osteopontin did not show any significant differences between the groups in terms of the extent of EF. The staging of EF based on vascular counts observed after immunohistochemical staining for CD105, but not for osteopontin, was compatible with conventional staging methods. Neither toxic effects on tissues nor systemic side effects were observed with the use of sorafenib. CONCLUSION Local administration of sorafenib significantly reduced post-laminectomy EF. Decreased neovascularization in spinal tissue may be due to the sorafenib-induced inhibition of vascular endothelial growth factor.展开更多
In this paper, the effects of profile shift in cylindrical helical gear mechanisms have been investigated with numerical and analytical calculations. The mathematical model for computer simulation of gears has been de...In this paper, the effects of profile shift in cylindrical helical gear mechanisms have been investigated with numerical and analytical calculations. The mathematical model for computer simulation of gears has been designed and the numerical calculations have been carried out. Analytical calculations have been made with an excel program which was designed at different profile shift coefficients for a selected mechanism. Analytical calculations of the same mechanism have been verified by using ANSYS 14.5. The results of analytical and numerical solutions have been compared to profile shift coefficients.展开更多
文摘AIM To determine if sorafenib, an antineoplastic agent, could prevent the development of spinal epidural fibrosis(EF). METHODS The study used CD105 and osteopontin antibodiesin an immunohistochemical approach to quantify EF that occurred as a consequence of laminectomy in rats. Wistar albino rats(n = 16) were divided into two groups: control(L1-2 level laminectomy only) and sorafenib treatment(L1-2 level laminectomy + topical sorafenib). The animals were euthanatized after 6 wk, and the EF tissues were examined for histopathological changes after immunohistochemical staining. The EF grades were assigned to the tissues, and the treatment and control groups were compared. RESULTS The EF thickness, inflammatory cell density, and arachnoid adherences determined by light microscopy were significantly higher in the control group compared to the sorafenib-treated group. Based on fibrosis scores, the extent of EF in the treatment group was significantly lower than in the controls. Immunohistochemical staining for CD105 to identify microvessels revealed that the EF grades based on vessel count were significantly lower in the treatment group. Staining for osteopontin did not show any significant differences between the groups in terms of the extent of EF. The staging of EF based on vascular counts observed after immunohistochemical staining for CD105, but not for osteopontin, was compatible with conventional staging methods. Neither toxic effects on tissues nor systemic side effects were observed with the use of sorafenib. CONCLUSION Local administration of sorafenib significantly reduced post-laminectomy EF. Decreased neovascularization in spinal tissue may be due to the sorafenib-induced inhibition of vascular endothelial growth factor.
文摘In this paper, the effects of profile shift in cylindrical helical gear mechanisms have been investigated with numerical and analytical calculations. The mathematical model for computer simulation of gears has been designed and the numerical calculations have been carried out. Analytical calculations have been made with an excel program which was designed at different profile shift coefficients for a selected mechanism. Analytical calculations of the same mechanism have been verified by using ANSYS 14.5. The results of analytical and numerical solutions have been compared to profile shift coefficients.
