期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Regulation of c-SMAC formation and AKT-mTOR signaling by the TSG101-IFT20 axis in CD4^(+)T cells
1
作者 Jiung Jeong in kang +11 位作者 Yumin Kim Keun Bon Ku Jang Hyun Park Hyun-Jin Kim Chae Won Kim Jeongwoo La Hi Eun Jung Hyeon Cheol Kim Young Joon Choi Jaeho Kim Joon Kim Heung Kyu Lee 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2023年第5期525-539,共15页
CD4^(+)T cells play major roles in the adaptive immune system,which requires antigen recognition,costimulation,and cytokines for its elaborate orchestration.Recent studies have provided new insight into the importance... CD4^(+)T cells play major roles in the adaptive immune system,which requires antigen recognition,costimulation,and cytokines for its elaborate orchestration.Recent studies have provided new insight into the importance of the supramolecular activation cluster(SMAC),which comprises concentric circles and is involved in the amplification of CD4^(+)T cell activation.However,the underlying mechanism of SMAC formation remains poorly understood.Here,we performed single-cell RNA sequencing of CD4^(+)T cells left unstimulated and stimulated with anti-CD3 and anti-CD28 antibodies to identify novel proteins involved in their regulation.We found that intraflagellar transport 20(IFT20),previously known as cilia-forming protein,was upregulated in antibody-stimulated CD4^(+)T cells compared to unstimulated CD4^(+)T cells.We also found that IFT20 interacted with tumor susceptibility gene 101(TSG101),a protein that endocytoses ubiquitinated T-cell receptors.The interaction between IFT20 and TSG101 promoted SMAC formation,which led to amplification of AKT-mTOR signaling.However,IFT20-deficient CD4^(+)T cells showed SMAC malformation,resulting in reduced CD4^(+)T cell proliferation,aerobic glycolysis,and cellular respiration.Finally,mice with T-cell-specific IFT20 deficiency exhibited reduced allergen-induced airway inflammation.Thus,our data suggest that the IFT20-TSG101 axis regulates AKT-mTOR signaling via SMAC formation. 展开更多
关键词 CD4^(+)T cell Intraflagellar transport 20 Tumor susceptibility gene 101 AKT-mTOR signaling Asthma Supramolecular activation cluster
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部