Hydroxyapatite,an essential mineral in human bones composed mainly of calcium and phosphorus,is widely used to coat bone graft and implant surfaces for enhanced biocompatibility and bone formation.For a strong implant...Hydroxyapatite,an essential mineral in human bones composed mainly of calcium and phosphorus,is widely used to coat bone graft and implant surfaces for enhanced biocompatibility and bone formation.For a strong implant-bone bond,the bone-forming cells must not only adhere to the implant surface but also move to the surface requiring bone formation.However,strong adhesion tends to inhibit cell migration on the surface of hydroxyapatite.Herein,a cell migration highway pattern that can promote cell migration was prepared using a nanosecond laser on hydroxyapatite coating.The developed surface promoted bone-forming cell movement compared with the unpatterned hydroxyapatite surface,and the cell adhesion and movement speed could be controlled by adjusting the pattern width.Live-cell microscopy,cell tracking,and serum protein analysis revealed the fundamental principle of this phenomenon.These findings are applicable to hydroxyapatite-coated biomaterials and can be implemented easily by laser patterning without complicated processes.The cell migration highway can promote and control cell movement while maintaining the existing advantages of hydroxyapatite coatings.Furthermore,it can be applied to the surface treatment of not only implant materials directly bonded to bone but also various implanted biomaterials implanted that require cell movement control.展开更多
基金This work was supported by a National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)[grant number 2020R1A2C2010413]the Korea Medical Device Development Fund grant funded by the Korea government(the Ministry of Science and ICT,the Ministry of Trade,Industry and Energy,the Ministry of Health&Welfare,the Ministry of Food and Drug Safety)(NTIS Number:9991007189)+1 种基金the KIST project(grant number 2E31121)the KU-KIST Graduate School of Converging Science and Technology Program.
文摘Hydroxyapatite,an essential mineral in human bones composed mainly of calcium and phosphorus,is widely used to coat bone graft and implant surfaces for enhanced biocompatibility and bone formation.For a strong implant-bone bond,the bone-forming cells must not only adhere to the implant surface but also move to the surface requiring bone formation.However,strong adhesion tends to inhibit cell migration on the surface of hydroxyapatite.Herein,a cell migration highway pattern that can promote cell migration was prepared using a nanosecond laser on hydroxyapatite coating.The developed surface promoted bone-forming cell movement compared with the unpatterned hydroxyapatite surface,and the cell adhesion and movement speed could be controlled by adjusting the pattern width.Live-cell microscopy,cell tracking,and serum protein analysis revealed the fundamental principle of this phenomenon.These findings are applicable to hydroxyapatite-coated biomaterials and can be implemented easily by laser patterning without complicated processes.The cell migration highway can promote and control cell movement while maintaining the existing advantages of hydroxyapatite coatings.Furthermore,it can be applied to the surface treatment of not only implant materials directly bonded to bone but also various implanted biomaterials implanted that require cell movement control.
