The observation of the sparkling discharges during the micro-arc oxidation process in KOH aqueous electrolyte was achieved. The change of surface morphology was progressively observed and a plausible pore formation me...The observation of the sparkling discharges during the micro-arc oxidation process in KOH aqueous electrolyte was achieved. The change of surface morphology was progressively observed and a plausible pore formation mechanism is proposed. Cell proliferation and ALP activity of micro- arc oxidized titanium was evaluated by human body derived osteoblasts and sligtaly better than those of blasted surface.展开更多
The modification of 3D printed porous titanium(Ti),especially for the internal pore structure,is critical and has received more attention to promoting its osteogenesis for clinical use.Ultra-violet(UV)responsive chito...The modification of 3D printed porous titanium(Ti),especially for the internal pore structure,is critical and has received more attention to promoting its osteogenesis for clinical use.Ultra-violet(UV)responsive chitosan(CSMA),as an injectable filling material,was firstly incorporated into porous Ti,and then CSMA was in-situ mineralized by carbon oxide(CO_(2))diffusion(CSMA/CaCO_(3)).Their physical-chemical and biological properties were investigated in vitro.CaCO_(3) crystals within CSMA hydrogels were successfully deposited into pores of porous Ti,which exhibited favorable biocompatibility.Ti implants filled with CSMA/CaCO_(3) promoted adhesion and proliferation of bone mesenchymal stem cells(BMSCs).Moreover,Ti implant filled CSMA/CaCO_(3) hydrogels could increase alkaline phosphatase(ALP)activities,up-regulate osteopontin(OPN)and osteocalcin(OCN)expression levels,and enhance extracellular mineralization.3D printed porous Ti filled with mineralized UV-responsive chitosan hydrogel could promote proliferation and osteogenesis of BMSCs,and have great potential for the modification of porous Ti implants in bone tissue engineering.展开更多
Three-dimensional printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues.In consideration of the disadvantages of bone sub...Three-dimensional printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues.In consideration of the disadvantages of bone substitutes,including poor mechanical properties,lack of vascularization and insufficient osteointegration,functional modification strategies can provide multiple functions and desired characteristics of printing materials,enhance their physicochemical and biological properties in bone tissue engineering.Thus,this review focuses on the advances of functional engineering strategies for 3D printed biomaterials in hard tissue replacement.It is structured as introducing 3D printing technologies,properties of printing materials(metals,ceramics and polymers)and typical functional engineering strategies utilized in the application of bone,cartilage and joint regeneration.展开更多
At the time of implanting bone-related implants into human body,a variety of biological responses to the material surface occur with respect to surface chemistry and physical state.The commonly used biomaterials(e.g.t...At the time of implanting bone-related implants into human body,a variety of biological responses to the material surface occur with respect to surface chemistry and physical state.The commonly used biomaterials(e.g.titanium and its alloy,Co–Cr alloy,stainless steel,polyetheretherketone,ultra-high molecular weight polyethylene and various calcium phosphates)have many drawbacks such as lack of biocompatibility and improper mechanical properties.As surface modification is very promising technology to overcome such problems,a variety of surface modification techniques have been being investigated.This review paper covers recent advances in surface modification techniques of bone-related materials including physicochemical coating,radiation grafting,plasma surface engineering,ion beam processing and surface patterning techniques.The contents are organized with different types of techniques to applicable materials,and typical examples are also described.展开更多
Dense hydroxyapatite （HA） ceramic is a promising material for hard tissue repair due to its unique physical properties and biologic properties. However, the brittleness and low compressive strength of traditional HA...Dense hydroxyapatite （HA） ceramic is a promising material for hard tissue repair due to its unique physical properties and biologic properties. However, the brittleness and low compressive strength of traditional HA ceramics limited their applications, because previous sintering methods produced HA ceramics with crystal sizes greater than nanometer range. In this study, nano-sized HA powder was employed to fabricate dense nanocrystal HA ceramic by high pressure molding, and followed by a three-step sintering process. The phase composition, microstructure, crystal dimension and crystal shape of the sintered ceramic were examined by X-ray diffraction （XRD） and scanning electron microscopy （SEM）. Mechanical properties of the HA ceramic were tested, and cytocompatibility was evaluated. The phase of the sintered ceramic was pure HA, and the crystal size was about 200 nm. The compressive strength and elastic modulus of the HA ceramic were comparable to human cortical bone, especially the good fatigue strength overcame brittleness of traditional sintered HA ceramics. Cell attachment experiment also demonstrated that the ceramics had a good cytocompatibility.展开更多
Hepatoma cells （Hepg2s） as typical cancer cells cultured on hydroxyl （-OH） and methyl （- CH3） group surfaces were shown to exhibit different proliferation and morphological changes. Hepg2s cells on OH surfaces g...Hepatoma cells （Hepg2s） as typical cancer cells cultured on hydroxyl （-OH） and methyl （- CH3） group surfaces were shown to exhibit different proliferation and morphological changes. Hepg2s cells on OH surfaces grew much more rapidly than those on -CH3 surfaces. Hepg2s cells on -OH surfaces had the larger contact area and the more flattened morphology, while those on CH3 surfaces exhibited the smaller contact area and the more rounded morphology. After 7 days of culture, the migration of Hepg2s cells into clusters on the CH3 surfaces behaved significantly slower than that on the OH surfaces. These chemically modified surfaces exhibited regulation of Hepg2s cells on proliferation, adhesion, and migration, providing a potential treatment of liver cancer.展开更多
Based on the last 10 successful series of the China–Korea Symposium on Biomaterials and Nano-biotechnology,we initiated a new invitation-based bilateral forum for established leaders and emerging young scientists in ...Based on the last 10 successful series of the China–Korea Symposium on Biomaterials and Nano-biotechnology,we initiated a new invitation-based bilateral forum for established leaders and emerging young scientists in the field,the 2014 China–Korea symposium on biomimetic and regenerative medical materials,which was held from November 26 to 28 in Wuhan,China.In the past decade,a lot of breakthrough achievements in biomedical materials and regenerative medicine have been made in our two countries.Currently,biomaterials science and engineering has been evolved into a critical stage of bioactive integration and tissue regeneration from a simple functional replacement and substitution.Biomimetic and regenerative medical materials will become main topics in the field.展开更多
文摘The observation of the sparkling discharges during the micro-arc oxidation process in KOH aqueous electrolyte was achieved. The change of surface morphology was progressively observed and a plausible pore formation mechanism is proposed. Cell proliferation and ALP activity of micro- arc oxidized titanium was evaluated by human body derived osteoblasts and sligtaly better than those of blasted surface.
基金financially supported partly by the Zhejiang Provincial Natural Science Foundation of China(No.LY20E010006)partly by the Fundamental Research Funds for the Central Universities(No.WK9110000152)+1 种基金partly by the Key Research and Development Plan of Anhui Province(No.20194a0720097)partly by the National Natural Science Foundation of China(Nos.51502265 and 81701033).
文摘The modification of 3D printed porous titanium(Ti),especially for the internal pore structure,is critical and has received more attention to promoting its osteogenesis for clinical use.Ultra-violet(UV)responsive chitosan(CSMA),as an injectable filling material,was firstly incorporated into porous Ti,and then CSMA was in-situ mineralized by carbon oxide(CO_(2))diffusion(CSMA/CaCO_(3)).Their physical-chemical and biological properties were investigated in vitro.CaCO_(3) crystals within CSMA hydrogels were successfully deposited into pores of porous Ti,which exhibited favorable biocompatibility.Ti implants filled with CSMA/CaCO_(3) promoted adhesion and proliferation of bone mesenchymal stem cells(BMSCs).Moreover,Ti implant filled CSMA/CaCO_(3) hydrogels could increase alkaline phosphatase(ALP)activities,up-regulate osteopontin(OPN)and osteocalcin(OCN)expression levels,and enhance extracellular mineralization.3D printed porous Ti filled with mineralized UV-responsive chitosan hydrogel could promote proliferation and osteogenesis of BMSCs,and have great potential for the modification of porous Ti implants in bone tissue engineering.
基金supported partly by the Zhejiang Provincial Natural Science Foundation of China(LY20E010006)partly by the National Natural Science Foundation of China(51502265 and 81701033).
文摘Three-dimensional printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues.In consideration of the disadvantages of bone substitutes,including poor mechanical properties,lack of vascularization and insufficient osteointegration,functional modification strategies can provide multiple functions and desired characteristics of printing materials,enhance their physicochemical and biological properties in bone tissue engineering.Thus,this review focuses on the advances of functional engineering strategies for 3D printed biomaterials in hard tissue replacement.It is structured as introducing 3D printing technologies,properties of printing materials(metals,ceramics and polymers)and typical functional engineering strategies utilized in the application of bone,cartilage and joint regeneration.
基金This work was partly supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education,Korea(2012R1A1A2040717)the National Basic Research Program of China funded by the Ministry of Science and Technology(MOST)of China(2011CB606205)+1 种基金the National Science and Technology Supporting Program of China funded by the MOST of China(2012BAI17B02)the National Natural Science Fund funded by the National Natural Science Foundation of China(21371106).
文摘At the time of implanting bone-related implants into human body,a variety of biological responses to the material surface occur with respect to surface chemistry and physical state.The commonly used biomaterials(e.g.titanium and its alloy,Co–Cr alloy,stainless steel,polyetheretherketone,ultra-high molecular weight polyethylene and various calcium phosphates)have many drawbacks such as lack of biocompatibility and improper mechanical properties.As surface modification is very promising technology to overcome such problems,a variety of surface modification techniques have been being investigated.This review paper covers recent advances in surface modification techniques of bone-related materials including physicochemical coating,radiation grafting,plasma surface engineering,ion beam processing and surface patterning techniques.The contents are organized with different types of techniques to applicable materials,and typical examples are also described.
文摘Dense hydroxyapatite （HA） ceramic is a promising material for hard tissue repair due to its unique physical properties and biologic properties. However, the brittleness and low compressive strength of traditional HA ceramics limited their applications, because previous sintering methods produced HA ceramics with crystal sizes greater than nanometer range. In this study, nano-sized HA powder was employed to fabricate dense nanocrystal HA ceramic by high pressure molding, and followed by a three-step sintering process. The phase composition, microstructure, crystal dimension and crystal shape of the sintered ceramic were examined by X-ray diffraction （XRD） and scanning electron microscopy （SEM）. Mechanical properties of the HA ceramic were tested, and cytocompatibility was evaluated. The phase of the sintered ceramic was pure HA, and the crystal size was about 200 nm. The compressive strength and elastic modulus of the HA ceramic were comparable to human cortical bone, especially the good fatigue strength overcame brittleness of traditional sintered HA ceramics. Cell attachment experiment also demonstrated that the ceramics had a good cytocompatibility.
文摘Hepatoma cells （Hepg2s） as typical cancer cells cultured on hydroxyl （-OH） and methyl （- CH3） group surfaces were shown to exhibit different proliferation and morphological changes. Hepg2s cells on OH surfaces grew much more rapidly than those on -CH3 surfaces. Hepg2s cells on -OH surfaces had the larger contact area and the more flattened morphology, while those on CH3 surfaces exhibited the smaller contact area and the more rounded morphology. After 7 days of culture, the migration of Hepg2s cells into clusters on the CH3 surfaces behaved significantly slower than that on the OH surfaces. These chemically modified surfaces exhibited regulation of Hepg2s cells on proliferation, adhesion, and migration, providing a potential treatment of liver cancer.
基金This work was supported by the National Natural Science Foundation of China[81461148032,51481340284,81471792]National Research Foundation of Korea[NRF-2014K2A2A1000942].
文摘Based on the last 10 successful series of the China–Korea Symposium on Biomaterials and Nano-biotechnology,we initiated a new invitation-based bilateral forum for established leaders and emerging young scientists in the field,the 2014 China–Korea symposium on biomimetic and regenerative medical materials,which was held from November 26 to 28 in Wuhan,China.In the past decade,a lot of breakthrough achievements in biomedical materials and regenerative medicine have been made in our two countries.Currently,biomaterials science and engineering has been evolved into a critical stage of bioactive integration and tissue regeneration from a simple functional replacement and substitution.Biomimetic and regenerative medical materials will become main topics in the field.
