Abstract： Viral diseases are an important limiting factor in many crop production systems in Azerbaijan. Symptomatic plants in main crop-producing areas were tested by ELISA （enzyme-linked immunosorbent assay） usin...Abstract： Viral diseases are an important limiting factor in many crop production systems in Azerbaijan. Symptomatic plants in main crop-producing areas were tested by ELISA （enzyme-linked immunosorbent assay） using specific monoclonal and polyclonal antibodies. Then RCA （rolling circle amplification） of circular DNA and PCR using different specific primer pairs have indicated that the tested symptomatic plant samples were completely infected by the following viruses： Luteovirus [BLRV （Bean leaf roll virus）], Potyviruses [BCMV （Bean common mosaic virus）, BYMV （Bean yellow mosaic virus）], Bromovirus [（AMV） Alfa-alfa mosaic virus], Geminiviruses [CpCDV （ Cickpea chlorotic dwarf virus） and TYLCV （Tomato yellow leaf curl virus）] and Nanoviruses [two different FBNYV （Faba bean necrotic yellow virus） and FBNSV （Faba bean necrotic stunt virus）]. At the same time generation sites of superoxide and hydrogen peroxide radicals and activity of antioxidant enzymes were studied in the naturally infected plants.展开更多
文摘Abstract： Viral diseases are an important limiting factor in many crop production systems in Azerbaijan. Symptomatic plants in main crop-producing areas were tested by ELISA （enzyme-linked immunosorbent assay） using specific monoclonal and polyclonal antibodies. Then RCA （rolling circle amplification） of circular DNA and PCR using different specific primer pairs have indicated that the tested symptomatic plant samples were completely infected by the following viruses： Luteovirus [BLRV （Bean leaf roll virus）], Potyviruses [BCMV （Bean common mosaic virus）, BYMV （Bean yellow mosaic virus）], Bromovirus [（AMV） Alfa-alfa mosaic virus], Geminiviruses [CpCDV （ Cickpea chlorotic dwarf virus） and TYLCV （Tomato yellow leaf curl virus）] and Nanoviruses [two different FBNYV （Faba bean necrotic yellow virus） and FBNSV （Faba bean necrotic stunt virus）]. At the same time generation sites of superoxide and hydrogen peroxide radicals and activity of antioxidant enzymes were studied in the naturally infected plants.
