Five horses with lesions compatible with dermatomycosis were selected and skin scrapings were taken from all affected areas. Samples were then treated with 10% KOH and observed by direct microscopic examination;fungal...Five horses with lesions compatible with dermatomycosis were selected and skin scrapings were taken from all affected areas. Samples were then treated with 10% KOH and observed by direct microscopic examination;fungal cultures using Mycobiotic Agar and Borelli Agar were also preformed;Trichophyton mentagrophytes was identified in two cases, whereas Candida albicans was identified in the other three. Skin lesions in cases 1 and 2 were treated topically with ketoconazol ointment, while in the remaining cases a weekly bath with propolis-based shampoo and application of propolis-based ointment on the lesions 2 to 3 times a week were implemented. Treatment in all cases lasted 4 weeks. Pictures were taken before and after treatment and, making use of the Image Pro-Express<sup>®</sup> software, therapy efficacy was determined by measurement of lesion area diameter and a significant reduction in lesion size was observed by the end of the 4 weeks of treatment with ketoconazole and propolis-based products. Thus proving the effectiveness of such propolis-based products, making them a natural therapeutic alternative for the treatment of cutaneous mycoses in horses, and carrying none of the toxic side effects of conventional pharmaceuticals.展开更多
Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Obje...Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Objectives: To establish an in vitro infection model of M. pachydermatis-exposed RK13 cells in order to evaluate cell morphological changes as well as changes in the structure of actin filaments. Methods: Cultures of RK13 cells were infected with M. pachydermatis, alterations caused by the yeast were evaluated by optical and fluorescence microscopy. Results: M. pachydermatis adheres itself to the cell and produces the formation of multiple agglomerates that cause changes in cell morphology, formation of cell aggregates in overlays, presence of syncytia and destruction of cell culture structure. The damaged cells presented changes in the actin filaments consisting of thickening of the cell cortex and loss of stress fibers. On the other hand, the formation of perinuclear actin rings in the yeasts was observed. Conclusions: An in vitro infection model was established with M. pachydermatis and alterations in cell morphology were observed consisting of changes in the structure of the actin filaments, overgrowth of the cells and the presence of syncytia.展开更多
文摘Five horses with lesions compatible with dermatomycosis were selected and skin scrapings were taken from all affected areas. Samples were then treated with 10% KOH and observed by direct microscopic examination;fungal cultures using Mycobiotic Agar and Borelli Agar were also preformed;Trichophyton mentagrophytes was identified in two cases, whereas Candida albicans was identified in the other three. Skin lesions in cases 1 and 2 were treated topically with ketoconazol ointment, while in the remaining cases a weekly bath with propolis-based shampoo and application of propolis-based ointment on the lesions 2 to 3 times a week were implemented. Treatment in all cases lasted 4 weeks. Pictures were taken before and after treatment and, making use of the Image Pro-Express<sup>®</sup> software, therapy efficacy was determined by measurement of lesion area diameter and a significant reduction in lesion size was observed by the end of the 4 weeks of treatment with ketoconazole and propolis-based products. Thus proving the effectiveness of such propolis-based products, making them a natural therapeutic alternative for the treatment of cutaneous mycoses in horses, and carrying none of the toxic side effects of conventional pharmaceuticals.
文摘Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Objectives: To establish an in vitro infection model of M. pachydermatis-exposed RK13 cells in order to evaluate cell morphological changes as well as changes in the structure of actin filaments. Methods: Cultures of RK13 cells were infected with M. pachydermatis, alterations caused by the yeast were evaluated by optical and fluorescence microscopy. Results: M. pachydermatis adheres itself to the cell and produces the formation of multiple agglomerates that cause changes in cell morphology, formation of cell aggregates in overlays, presence of syncytia and destruction of cell culture structure. The damaged cells presented changes in the actin filaments consisting of thickening of the cell cortex and loss of stress fibers. On the other hand, the formation of perinuclear actin rings in the yeasts was observed. Conclusions: An in vitro infection model was established with M. pachydermatis and alterations in cell morphology were observed consisting of changes in the structure of the actin filaments, overgrowth of the cells and the presence of syncytia.
