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Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An in vitro study 被引量:4
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作者 Dipanwita Das Neelakshi Sarkar +7 位作者 isha sengupta Ananya Pal Debraj Saha Manikankana Bandopadhyay Chandrima Das Jimmy Narayan Shivram Prasad Singh Runu Chakravarty 《World Journal of Gastroenterology》 SCIE CAS 2016年第47期10341-10352,共12页
AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time ... AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the Hep G2.2.15 cells with TLR4 specific ligand. Nuclear factor(NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.RESULTS The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.CONCLUSION The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future. 展开更多
关键词 Hepatitis B virus Toll like receptor 4 Cell cycle Epigenetic marks Innate immune response
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