Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened ...Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using～1H and～(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries.展开更多
A new metabolite,named seimatoric acid（1）,representing a new oxobutanoic acid derivative has been isolated from Seimatosporium sp., in addition to four known compounds viz.,2-hydroxymethyl-4β,5α,6β-trihydroxycycl...A new metabolite,named seimatoric acid（1）,representing a new oxobutanoic acid derivative has been isolated from Seimatosporium sp., in addition to four known compounds viz.,2-hydroxymethyl-4β,5α,6β-trihydroxycyclohex-2-enone（2）,（-）-phyllostine（3）,（＋）-epiepoxydon（4） and（＋）-epoxydon monoacetate（5）.Similarly one new benzoic acid derivative,named colletonoic acid（6） was isolated from the ethyl acetate fraction of Colletotrichum sp.The structures of the new compounds were elucidated by detailed ^1 H NMR,^13C NMR,COSY,HMQC.HMBC spectroscopic analysis,and HR-E1-MS.Seimatoric acid（1）was also isolated from another taxonomical unidentified fungal strain 4295 in ourgroup.The structures of the known compounds were elucidated by their spectral data comparison to literature data.Preliminary studies showed that colletonoic acid（6） showed good antibacterial,antifungal,and antialgal activities.展开更多
基金supported by the BMBF(Bundesministerium fr Bildung und Forschung,project no.03F0360A)
文摘Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using～1H and～(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries.
基金BASF AG and the BMBF(Bundesministerium für Bildung und Forschung,No.03F0360A)
文摘A new metabolite,named seimatoric acid（1）,representing a new oxobutanoic acid derivative has been isolated from Seimatosporium sp., in addition to four known compounds viz.,2-hydroxymethyl-4β,5α,6β-trihydroxycyclohex-2-enone（2）,（-）-phyllostine（3）,（＋）-epiepoxydon（4） and（＋）-epoxydon monoacetate（5）.Similarly one new benzoic acid derivative,named colletonoic acid（6） was isolated from the ethyl acetate fraction of Colletotrichum sp.The structures of the new compounds were elucidated by detailed ^1 H NMR,^13C NMR,COSY,HMQC.HMBC spectroscopic analysis,and HR-E1-MS.Seimatoric acid（1）was also isolated from another taxonomical unidentified fungal strain 4295 in ourgroup.The structures of the known compounds were elucidated by their spectral data comparison to literature data.Preliminary studies showed that colletonoic acid（6） showed good antibacterial,antifungal,and antialgal activities.
