The present study reports bioactive constituents from biquinho pepper,such as carotenoids,capsaicinoids,and capsinoids,identified by UHPLC-MS.Biquinho pepper extract showed an antioxidant potential by DPPH(33.17±...The present study reports bioactive constituents from biquinho pepper,such as carotenoids,capsaicinoids,and capsinoids,identified by UHPLC-MS.Biquinho pepper extract showed an antioxidant potential by DPPH(33.17±0.08%),ORAC(32.35±0.02μM TE/g),β-carotene/linoleic acid(55.80±1.30%),and FRAP(315.88±4.23μmol TE/g)assays.Additionally,in vivo antioxidant analyses demonstrated that the pepper extract(170μg/mL)decreased the damage promoted by H_(2)O_(2) in Saccharomyces cerevisiae cells.Fish balls were prepared using different concentrations of pepper(0.0%,0.5%,0.75%,1.0%)to investigate its antioxidant effect during frozen storage.Biquinho pepper effectively reduced the formation of cholesterol oxides and preserved essential fatty acids,mainly in samples containing 1.0%of pepper.Moreover,this treatment was more efficient than sodium erythorbate(p<0.05),which was used as standard.The kinetics of cholesterol oxidation was also determined,showing that both cholesterol degradation and cholesterol oxides formation fit a first-order kinetic model(R^(2)>0.8).展开更多
基金the Natl.Brazilian Research Foundations(CAPES)for the scholarship,S˜ao Paulo Research Foundation(FAPESP)Grant 2008/58035-6 for the use of the UHPLC-MS equipmentthe FAPERJ for financial support(Grant E-26/010.000857/2015).
文摘The present study reports bioactive constituents from biquinho pepper,such as carotenoids,capsaicinoids,and capsinoids,identified by UHPLC-MS.Biquinho pepper extract showed an antioxidant potential by DPPH(33.17±0.08%),ORAC(32.35±0.02μM TE/g),β-carotene/linoleic acid(55.80±1.30%),and FRAP(315.88±4.23μmol TE/g)assays.Additionally,in vivo antioxidant analyses demonstrated that the pepper extract(170μg/mL)decreased the damage promoted by H_(2)O_(2) in Saccharomyces cerevisiae cells.Fish balls were prepared using different concentrations of pepper(0.0%,0.5%,0.75%,1.0%)to investigate its antioxidant effect during frozen storage.Biquinho pepper effectively reduced the formation of cholesterol oxides and preserved essential fatty acids,mainly in samples containing 1.0%of pepper.Moreover,this treatment was more efficient than sodium erythorbate(p<0.05),which was used as standard.The kinetics of cholesterol oxidation was also determined,showing that both cholesterol degradation and cholesterol oxides formation fit a first-order kinetic model(R^(2)>0.8).
