Genotype independent transformation andregeneration of Indian cotton(Gossypiumhirsutum L.)cultivar was standardized with Bt-Cry 1A(b)gene by Agrobacterium-mediation.Apical meristem of elite G.hirsutum cultivarLRK-516 ...Genotype independent transformation andregeneration of Indian cotton(Gossypiumhirsutum L.)cultivar was standardized with Bt-Cry 1A(b)gene by Agrobacterium-mediation.Apical meristem of elite G.hirsutum cultivarLRK-516 and LRA 5166 were co-cultivated withA.tumefaciens LBA 4404 carrying synthetic Bt-展开更多
RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.Th...RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The展开更多
文摘Genotype independent transformation andregeneration of Indian cotton(Gossypiumhirsutum L.)cultivar was standardized with Bt-Cry 1A(b)gene by Agrobacterium-mediation.Apical meristem of elite G.hirsutum cultivarLRK-516 and LRA 5166 were co-cultivated withA.tumefaciens LBA 4404 carrying synthetic Bt-
文摘RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The
