巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发...巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。展开更多
This study was conducted wiffl a spring soybean variety Jindou 19 to test file effects of rhizobia inoculation or non-inoculation and nitrogen application rates at 0, 27, 54 and 75 kg/hm^2 (N1-4) on root nodule dry ...This study was conducted wiffl a spring soybean variety Jindou 19 to test file effects of rhizobia inoculation or non-inoculation and nitrogen application rates at 0, 27, 54 and 75 kg/hm^2 (N1-4) on root nodule dry weight, root nodule number, yield and yield components of soybean. The results showed that the nitrogen fertilizer significantly affected soybean root noduce and soybean yield, the dry weight and number of root nodules increased gradually with the increase of nitrogen application rate, and the yield of file rhizobia inoculated treatment was significantly higher than that of file rhizobia non-inoculated treatment; and under file condition of file rhizobia inoculation, with nitrogen application amount at 75 kg/hm^2, file Jindou 19 performed a better nitrogen fixing effect in file growing stage, and its plot yield was 4 997.417 kg/hm^2, 4.06% higher than that of file treatment without inoculating rhizobium and 12.50% higher than that of the treatment without applying nitrogen fertilizer.展开更多
文摘巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。
文摘This study was conducted wiffl a spring soybean variety Jindou 19 to test file effects of rhizobia inoculation or non-inoculation and nitrogen application rates at 0, 27, 54 and 75 kg/hm^2 (N1-4) on root nodule dry weight, root nodule number, yield and yield components of soybean. The results showed that the nitrogen fertilizer significantly affected soybean root noduce and soybean yield, the dry weight and number of root nodules increased gradually with the increase of nitrogen application rate, and the yield of file rhizobia inoculated treatment was significantly higher than that of file rhizobia non-inoculated treatment; and under file condition of file rhizobia inoculation, with nitrogen application amount at 75 kg/hm^2, file Jindou 19 performed a better nitrogen fixing effect in file growing stage, and its plot yield was 4 997.417 kg/hm^2, 4.06% higher than that of file treatment without inoculating rhizobium and 12.50% higher than that of the treatment without applying nitrogen fertilizer.