Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendo...Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendorff model and to discuss the mechanisms. SD rat were randomly divided into 4groups: control group(perfused with KH buffer),10^(-4)mol·L^(-1),5×10^(-4)mol·L^(-1),and 10^(-3)mol·L^(-1)(perfused with betaine in different concentration respectively for 10 min before ischemia followed by KH buffer perfused). During the experiment we detected LVEDP,LVDP,dp/dt,and store the tissue to do western blot and TTC staining. The result showed that in control group the recovery rate is 19.6%,after pretreatment of betaine the recovery rate all increased significantly and in a dose-dependent manner. The LVEDP is markedly decreased in betaine treatment groups compare to control group. Coronary flow only in highest betaine treatment group(10^(-3)mol·L^-) is markedly increased compare to control group,other two groups have no change. Western blotting showed that SOD level is significantly increased in betaine treatment group compare to control group and in a dose dependent manner. TTC staining suggested that infarction size is reduced in betaine treatment group compare to control group. Above all,the results suggest that betaine have protect effect on I/R injury in heart and this effect may relate to ROS signaling pathway.展开更多
Curcum in, a dietary phytochemical, exhibits multifunctional natural product with regulatory effects on inflammation. However, the poor bioavailability limits its clinical applications. Thus, we designed and synthesiz...Curcum in, a dietary phytochemical, exhibits multifunctional natural product with regulatory effects on inflammation. However, the poor bioavailability limits its clinical applications. Thus, we designed and synthesized a novel monocarbonyl analogue of curcumin B7 and their inhibition against monocyte chemotactic protein-1(MCP-1) and interleukin-8(IL-8) release was evaluated in H_2O_2-stimulated human vascular endothelial cells(ECs) in a dose-responsive manner, while exhibiting no cytotoxicity in ECs. Taken together, these insights on the novel compound B7 may serve as potential agents for the treatment of atherosclerosis.展开更多
基金The project supported by National Natural Science Foundation of China(81400182)National Training Programs of Innovation and Entrepreneurship for Undergraduates(201610439129)
文摘Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendorff model and to discuss the mechanisms. SD rat were randomly divided into 4groups: control group(perfused with KH buffer),10^(-4)mol·L^(-1),5×10^(-4)mol·L^(-1),and 10^(-3)mol·L^(-1)(perfused with betaine in different concentration respectively for 10 min before ischemia followed by KH buffer perfused). During the experiment we detected LVEDP,LVDP,dp/dt,and store the tissue to do western blot and TTC staining. The result showed that in control group the recovery rate is 19.6%,after pretreatment of betaine the recovery rate all increased significantly and in a dose-dependent manner. The LVEDP is markedly decreased in betaine treatment groups compare to control group. Coronary flow only in highest betaine treatment group(10^(-3)mol·L^-) is markedly increased compare to control group,other two groups have no change. Western blotting showed that SOD level is significantly increased in betaine treatment group compare to control group and in a dose dependent manner. TTC staining suggested that infarction size is reduced in betaine treatment group compare to control group. Above all,the results suggest that betaine have protect effect on I/R injury in heart and this effect may relate to ROS signaling pathway.
基金National Natural Science Foundation of Chinagrant number:30800449+1 种基金Scientific Research Foundation for University of South Chinagrant number:2011XQD11
文摘Curcum in, a dietary phytochemical, exhibits multifunctional natural product with regulatory effects on inflammation. However, the poor bioavailability limits its clinical applications. Thus, we designed and synthesized a novel monocarbonyl analogue of curcumin B7 and their inhibition against monocyte chemotactic protein-1(MCP-1) and interleukin-8(IL-8) release was evaluated in H_2O_2-stimulated human vascular endothelial cells(ECs) in a dose-responsive manner, while exhibiting no cytotoxicity in ECs. Taken together, these insights on the novel compound B7 may serve as potential agents for the treatment of atherosclerosis.
