Objective： To explore the effects and possible mechanisms of Guiqi Oral Liquid （归芪口服液, GQOL） on the recovery of hematopoiesis in acute irradiation injured mice. Methods： The acute irradiation injured mice wer...Objective： To explore the effects and possible mechanisms of Guiqi Oral Liquid （归芪口服液, GQOL） on the recovery of hematopoiesis in acute irradiation injured mice. Methods： The acute irradiation injured mice were randomly divided into 2 groups： the treated group and the control group, and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy ^60Coγ-ray, every one of them was given 0.4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th, 8th and 14th day after irradiation, the bone marrow mononuclear cells （BMMNC） and megakaryocytes in bone marrow tissues of the mice were counted, the proportion of hematopoietic tissues （by area） was measured, and the expression of adhesion molecules, CD44 and CD54, in bone marrow were estimated by immunochemistry. The colony forming unit of spleen （CFU-S） in the mice were counted on the 8th day after irradiation. Results： On the 4th, 8th, 14th day after irradiation, the count of BMMNC and megakaryocyte, and the proportion of hematopoietic tissues in the treated group were higher than those in the control group （P〈0.01 or P〈0.05）. CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day （P〈0.01）, but near normal on the 14th day （P〈0.01）. On the 8th day, CFU-S count in the treated group was higher than that in the control group （P〈0.01）. Conclusion： GQOL can regulate the expression of adhesion molecules, CD44 and CD54, in the bone marrow of the acute irradiation injured mice, which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice.展开更多
The elegant 2D multiquantum (MQ) MAS NMR has been applied to investigate the coordination of Al atoms in SAPO-34. The results show that the effects of the template and/or water on the coordination of Al atoms are sig...The elegant 2D multiquantum (MQ) MAS NMR has been applied to investigate the coordination of Al atoms in SAPO-34. The results show that the effects of the template and/or water on the coordination of Al atoms are significant, which lead to the formation of various Al species. Up to four kinds of Al species are distinctly resolved.展开更多
Poly(e-caprolactone) (PCL) with weight-average molar mass over 10000 g/mol was synthesized by microwave-assisted ring-opening polymerization of e-caprolactone (e-CL) with maleic acid (MA) as initiator (2.45 GHz, 360 W...Poly(e-caprolactone) (PCL) with weight-average molar mass over 10000 g/mol was synthesized by microwave-assisted ring-opening polymerization of e-caprolactone (e-CL) with maleic acid (MA) as initiator (2.45 GHz, 360 W, 85 min). Ibuprofen-PCL controlled release system was prepared directly by the ROP of e-CL in its mixture with ibuprofen. The release of ibuprofen from the system was sustained and steady.展开更多
The chemical structure of an exopolysaccharide (EPS) produced by Sorangium cellulosum NUST06 was investigated. The EPS was found to have molecular masses greater than 2?106 Da, and consisted of D-glucose, D-mannose an...The chemical structure of an exopolysaccharide (EPS) produced by Sorangium cellulosum NUST06 was investigated. The EPS was found to have molecular masses greater than 2?106 Da, and consisted of D-glucose, D-mannose and D-glucuronic acid in a ratio of 5:4:1. The analytical results of methylation and nuclear magnetic resonance spectra suggested the EPS consist of following tetrasaccharide repeating units: 4) -b-D-Glup (1 4)- b-D-Glup (1 4) -b-D-Glup (1 a-D-GluAp (1 6) a-D-manp (1 6)-a-D-manp (1 6) (6) (4) (4) ↑ ↑ ↑ Me Ac Ac展开更多
In the context of workflow systems, security-relevant aspect is related to the assignment of activities to (human or automated) agents. This paper intends to cast light on the management of project-oriented workflow. ...In the context of workflow systems, security-relevant aspect is related to the assignment of activities to (human or automated) agents. This paper intends to cast light on the management of project-oriented workflow. A comprehensive authorization model is proposed from the perspective of project management. In this model, the concept of activity decomposition and team is introduced, which improves the security of conventional role-based access control. Furthermore, policy is provided to define the static and dynamic constraints such as Separation of Duty (SoD). Validity of constraints is proposed to provide a fine-grained assignment, which improves the performance of policy management. The model is applicable not only to project-oriented workflow applications but also to other teamwork environments such as virtual enterprise.展开更多
Promoter-probe vector pSUPV4 is used to clone the promoter of Pseudomonas pseudoalcaligenes directly in E. coli, and the recombinant pPA7, which has the highest kanamycin resistance, is obtained. Sequencing the PA7 fr...Promoter-probe vector pSUPV4 is used to clone the promoter of Pseudomonas pseudoalcaligenes directly in E. coli, and the recombinant pPA7, which has the highest kanamycin resistance, is obtained. Sequencing the PA7 fragment discloses several motifs similar to the conservative domains of prokaryotic promoters, including -10 box, -35 box, parallel SD fragment essential to transcription initiation, and the translation initiation site ATG. Southern blotting of PA7 indicates that the PA7 fragment comes from P. pseudoalcaligenes genome and has probably one copy. The PA7 fragment is subcloned by PCR, and the result shows that the 5’-flanking fragment from 889 to 1120 bp has promoter activity, which can be enhanced by the 0.7Kb fragment at 5’ end. The fragments of pPA7 and pPA7-2 are transferred into pseudomonas pseudoaligenes by electroporation, and the significant higher kanamycin resistance of transformants than that of control indicates that the PA7 fragment has the promoter activity in P. pseudoaligene.展开更多
Postmitotic neurons in the developing cortex migrate along radial glial fibers to their proper location in the cortical plate and form the layered structure.Here we report that the radial migration of rat layer II/III...Postmitotic neurons in the developing cortex migrate along radial glial fibers to their proper location in the cortical plate and form the layered structure.Here we report that the radial migration of rat layer II/III cortical neurons requires guidance by the extracellular diffusible factor Semaphorin-3A(Sema3A).This factor is expressed in a descending gradient across the cortical layers,whereas its receptor neuropilin-1(NP1)is highly expressed in migrating neurons.Downregulation or conditional knockout of NP1 in newborn cortical neurons impedes their radial migration by disrupting their radial orientation during migration without altering their cell fate.Studies in cultured cortical slices further show that the endogenous gradient of Sema3A is required for the proper migration of newborn neurons.In addition,transwell chemotaxis assays show that isolated newborn neurons are attracted by Sema3A.Thus,Sema3A may function as a chemoattractive guidance signal for the radial migration of newborn cortical neurons toward upper layers.展开更多
基金Supported by the National Natural Science Foundation ofChina (No .39870926)
文摘Objective： To explore the effects and possible mechanisms of Guiqi Oral Liquid （归芪口服液, GQOL） on the recovery of hematopoiesis in acute irradiation injured mice. Methods： The acute irradiation injured mice were randomly divided into 2 groups： the treated group and the control group, and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy ^60Coγ-ray, every one of them was given 0.4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th, 8th and 14th day after irradiation, the bone marrow mononuclear cells （BMMNC） and megakaryocytes in bone marrow tissues of the mice were counted, the proportion of hematopoietic tissues （by area） was measured, and the expression of adhesion molecules, CD44 and CD54, in bone marrow were estimated by immunochemistry. The colony forming unit of spleen （CFU-S） in the mice were counted on the 8th day after irradiation. Results： On the 4th, 8th, 14th day after irradiation, the count of BMMNC and megakaryocyte, and the proportion of hematopoietic tissues in the treated group were higher than those in the control group （P〈0.01 or P〈0.05）. CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day （P〈0.01）, but near normal on the 14th day （P〈0.01）. On the 8th day, CFU-S count in the treated group was higher than that in the control group （P〈0.01）. Conclusion： GQOL can regulate the expression of adhesion molecules, CD44 and CD54, in the bone marrow of the acute irradiation injured mice, which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice.
基金We are grateful to the support of the National Natural Science Foundation of China and the Ministry of Science and Technology of China. We appreciate Dr. R. Fu (NHMFL Tallahassee) for his kind help regarding the MQ MAS NMR experiments.
文摘The elegant 2D multiquantum (MQ) MAS NMR has been applied to investigate the coordination of Al atoms in SAPO-34. The results show that the effects of the template and/or water on the coordination of Al atoms are significant, which lead to the formation of various Al species. Up to four kinds of Al species are distinctly resolved.
基金This work was financially supported by the Research Foundation of MOE and National 973 Project of China (G1999064703).
文摘Poly(e-caprolactone) (PCL) with weight-average molar mass over 10000 g/mol was synthesized by microwave-assisted ring-opening polymerization of e-caprolactone (e-CL) with maleic acid (MA) as initiator (2.45 GHz, 360 W, 85 min). Ibuprofen-PCL controlled release system was prepared directly by the ROP of e-CL in its mixture with ibuprofen. The release of ibuprofen from the system was sustained and steady.
文摘The chemical structure of an exopolysaccharide (EPS) produced by Sorangium cellulosum NUST06 was investigated. The EPS was found to have molecular masses greater than 2?106 Da, and consisted of D-glucose, D-mannose and D-glucuronic acid in a ratio of 5:4:1. The analytical results of methylation and nuclear magnetic resonance spectra suggested the EPS consist of following tetrasaccharide repeating units: 4) -b-D-Glup (1 4)- b-D-Glup (1 4) -b-D-Glup (1 a-D-GluAp (1 6) a-D-manp (1 6)-a-D-manp (1 6) (6) (4) (4) ↑ ↑ ↑ Me Ac Ac
文摘In the context of workflow systems, security-relevant aspect is related to the assignment of activities to (human or automated) agents. This paper intends to cast light on the management of project-oriented workflow. A comprehensive authorization model is proposed from the perspective of project management. In this model, the concept of activity decomposition and team is introduced, which improves the security of conventional role-based access control. Furthermore, policy is provided to define the static and dynamic constraints such as Separation of Duty (SoD). Validity of constraints is proposed to provide a fine-grained assignment, which improves the performance of policy management. The model is applicable not only to project-oriented workflow applications but also to other teamwork environments such as virtual enterprise.
文摘Promoter-probe vector pSUPV4 is used to clone the promoter of Pseudomonas pseudoalcaligenes directly in E. coli, and the recombinant pPA7, which has the highest kanamycin resistance, is obtained. Sequencing the PA7 fragment discloses several motifs similar to the conservative domains of prokaryotic promoters, including -10 box, -35 box, parallel SD fragment essential to transcription initiation, and the translation initiation site ATG. Southern blotting of PA7 indicates that the PA7 fragment comes from P. pseudoalcaligenes genome and has probably one copy. The PA7 fragment is subcloned by PCR, and the result shows that the 5’-flanking fragment from 889 to 1120 bp has promoter activity, which can be enhanced by the 0.7Kb fragment at 5’ end. The fragments of pPA7 and pPA7-2 are transferred into pseudomonas pseudoaligenes by electroporation, and the significant higher kanamycin resistance of transformants than that of control indicates that the PA7 fragment has the promoter activity in P. pseudoaligene.
文摘Postmitotic neurons in the developing cortex migrate along radial glial fibers to their proper location in the cortical plate and form the layered structure.Here we report that the radial migration of rat layer II/III cortical neurons requires guidance by the extracellular diffusible factor Semaphorin-3A(Sema3A).This factor is expressed in a descending gradient across the cortical layers,whereas its receptor neuropilin-1(NP1)is highly expressed in migrating neurons.Downregulation or conditional knockout of NP1 in newborn cortical neurons impedes their radial migration by disrupting their radial orientation during migration without altering their cell fate.Studies in cultured cortical slices further show that the endogenous gradient of Sema3A is required for the proper migration of newborn neurons.In addition,transwell chemotaxis assays show that isolated newborn neurons are attracted by Sema3A.Thus,Sema3A may function as a chemoattractive guidance signal for the radial migration of newborn cortical neurons toward upper layers.
