CD36 is a highly glycosylated integral membrane protein that belongs to the scavenger receptor class B family and regulates the pathological progress of metabolic diseases.CD36 was recently found to be widely expresse...CD36 is a highly glycosylated integral membrane protein that belongs to the scavenger receptor class B family and regulates the pathological progress of metabolic diseases.CD36 was recently found to be widely expressed in various cell types in the nervous system,including endothelial cells,pericytes,astrocytes,and microglia.CD36 mediates a number of regulatory processes,such as endothelial dysfunction,oxidative stress,mitochondrial dysfunction,and inflammatory responses,which are involved in many central nervous system diseases,such as stroke,Alzheimer’s disease,Parkinson’s disease,and spinal cord injury.CD36 antagonists can suppress CD36 expression or prevent CD36 binding to its ligand,thereby achieving inhibition of CD36-mediated pathways or functions.Here,we reviewed the mechanisms of action of CD36 antagonists,such as Salvianolic acid B,tanshinone IIA,curcumin,sulfosuccinimidyl oleate,antioxidants,and small-molecule compounds.Moreover,we predicted the structures of binding sites between CD36 and antagonists.These sites can provide targets for more efficient and safer CD36 antagonists for the treatment of central nervous system diseases.展开更多
A real-time adaptive roles allocation method based on reinforcement learning is proposed to improve humanrobot cooperation performance for a curtain wall installation task.This method breaks the traditional idea that ...A real-time adaptive roles allocation method based on reinforcement learning is proposed to improve humanrobot cooperation performance for a curtain wall installation task.This method breaks the traditional idea that the robot is regarded as the follower or only adjusts the leader and the follower in cooperation.In this paper,a self-learning method is proposed which can dynamically adapt and continuously adjust the initiative weight of the robot according to the change of the task.Firstly,the physical human-robot cooperation model,including the role factor is built.Then,a reinforcement learningmodel that can adjust the role factor in real time is established,and a reward and actionmodel is designed.The role factor can be adjusted continuously according to the comprehensive performance of the human-robot interaction force and the robot’s Jerk during the repeated installation.Finally,the roles adjustment rule established above continuously improves the comprehensive performance.Experiments of the dynamic roles allocation and the effect of the performance weighting coefficient on the result have been verified.The results show that the proposed method can realize the role adaptation and achieve the dual optimization goal of reducing the sum of the cooperator force and the robot’s Jerk.展开更多
The subfamily Megophryinae,as a representative batrachian group of the Oriental Realm and one of the most diverse groups of amphibians,has attracted considerable attention due to continued conjecture regarding its gen...The subfamily Megophryinae,as a representative batrachian group of the Oriental Realm and one of the most diverse groups of amphibians,has attracted considerable attention due to continued conjecture regarding its generic classification and failure to reach a satisfactory consensus.China boasts the richest diversity of Asian horned toads,containing some two thirds of the total species cataloged.However,most species have a complicated taxonomic history,resulting in multiple misidentifications.As such,an overall clarification of historical records and regional checklists is required.In the current investigation,we established the phylogeny of the Asian horned toads and performed detailed examinations with redefinitions of several important morphological traits.Based on the phylogenetic relationships and morphological differences,we propose a new ten-genus classification for the Asian horned toad subfamily Megophryinae:i.e.,Brachytarsophrys,Atympanophrys,Grillitschia,Sarawakiphrys gen.nov.,Jingophrys gen.nov.,Xenophrys,Megophrys,Pelobatrachus,Ophryophryne,and Boulenophrys.Revisions on the diagnosability,distribution,and content of each genus are provided.Furthermore,we present a careful review of the taxonomic history of Asian horned toad species from China and provide a monograph of congeners,including six species of Brachytarsophrys,four species of Atympanophrys,five species of Jingophrys gen.nov.,10 species of Xenophrys,two species of Ophryophryne,and 60 species of Boulenophrys.Finally,we discuss the importance of traditional morphological traits based on multiple populations in taxonomic work as well as taxonomic inflation caused by the genetic species delimitation.展开更多
The unconfined compressive strength(UCS)of alkali-activated slag(AAS)-based cemented paste backfill(CPB)is influenced by multiple design parameters.However,the experimental methods are limited to understanding the rel...The unconfined compressive strength(UCS)of alkali-activated slag(AAS)-based cemented paste backfill(CPB)is influenced by multiple design parameters.However,the experimental methods are limited to understanding the relationships between a single design parameter and the UCS,independently of each other.Although machine learning(ML)methods have proven efficient in understanding relationships between multiple parameters and the UCS of ordinary Portland cement(OPC)-based CPB,there is a lack of ML research on AAS-based CPB.In this study,two ensemble ML methods,comprising gradient boosting regression(GBR)and random forest(RF),were built on a dataset collected from literature alongside two other single ML methods,support vector regression(SVR)and artificial neural network(ANN).The results revealed that the ensemble learning methods outperformed the single learning methods in predicting the UCS of AAS-based CPB.Relative importance analysis based on the bestperforming model(GBR)indicated that curing time and water-to-binder ratio were the most critical input parameters in the model.Finally,the GBR model with the highest accuracy was proposed for the UCS predictions of AAS-based CPB.展开更多
The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote stru...The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote structural and functional recovery after spinal cord injury.Previous studies have shown that peptides can promote substantial repair and regeneration of injured tissue.While amphibians have a pronounced ability to regenerate the spinal cord,few studies have investigated the effect of amphibian spinal cord-derived peptides on spinal cord injury.Here we report for the first time the successful identification and isolation of a new polypeptide,VD11(amino acid sequence:VDELWPPWLPC),from the spinal cord of an endemic Chinese amphibian(Odorrana schmackeri).In vitro experiments showed that VD11 promoted the secretion of nerve growth factor and brain-derived neurotrophic factor in BV2 cells stimulated with lipopolysaccharide,as well as the proliferation and synaptic elongation of PC12 cells subjected to hypoxia.In vivo experiments showed that intravertebral injection of VD11 markedly promoted recovery of motor function in rats with spinal cord injury,alleviated pathological damage,and promoted axonal regeneration.Furthermore,RNA sequencing and western blotting showed that VD11 may affect spinal cord injury through activation of the AMPK and AKT signaling pathways.In summary,we discovered a novel amphibian-derived peptide that promotes structural and functional recovery after spinal cord injury.展开更多
An improved rheo-optic in situ synchronous measurement system was employed to investigate the gelation behaviour and mechanism of waxy crude oil emulsions. By combining transmitted natural light and reflected polarize...An improved rheo-optic in situ synchronous measurement system was employed to investigate the gelation behaviour and mechanism of waxy crude oil emulsions. By combining transmitted natural light and reflected polarized light microscopy, a multiangle composite light source was built to achieve the simultaneous observation of wax crystals and emulsified water droplets, as well as their dynamic aggregation process. Main outcomes on the microscopic mechanism were obtained by developed microscopic image processing method. It was found that the microstructure of W/O waxy crude oil emulsion has the evolution of “individual structure--homogeneous aggregate structure--heterogeneous coaggregate structure--floc structure” during the static cooling, which results in the four stages during gelation process. Different from previous studies, the aggregation of emulsified water droplets was found to be more significant and contributes to the formation and development of the wax crystals-emulsified water droplets coaggregate, which plays a decisive role in the further evolution of the gelled microstructure. Time series microscopic images show the dynamic aggregation of emulsified water droplets and wax crystals. Two different aggregation behaviours between wax crystals and water droplets were observed. That wax crystals can not only embed in gaps between adjacent water droplets and enhance the structure, but also surround the outside of the water droplets and continue to grow resulting in the interconnection of different coaggregates to form a larger floc structure. In addition, correlation between viscoelasticity and microstructure evolution of waxy crude oil emulsions of different water contents was discussed. With increasing water contents, the microstructure is changed from wax crystal flocculation structure as the main skeleton and the emulsified water droplets embedded in it, into the aggregation of emulsified water droplets occupying the main position. When the number of wax crystals and water droplets reaches a certain ratio, did wax crystals form coaggregates with emulsified water droplets, and the remaining wax crystals formed an overall flocculation structure, the viscoelasticity of the waxy crude oil emulsion is the highest.展开更多
The poor rate capability and low capacity are huge barriers to realize the commercial applications of battery-type transition metal compounds(TMCs) cathode.Herein,numerous Se vacancy defects are introduced into the Ni...The poor rate capability and low capacity are huge barriers to realize the commercial applications of battery-type transition metal compounds(TMCs) cathode.Herein,numerous Se vacancy defects are introduced into the Ni_(3)Se_(2)lamellas by pre-lithiation technique,which can be acted as a novel class of battery-type cathode for hybrid supercapacitors.Appropriately modulating the contents of the preembedded lithium(Li) ions can induce a controllable vacancy content in the series of as-prepared products,effectively endowing a fast reaction kinetic and high activity for the cathode.Benefiting from the distinct design,the optimized cathode(Li2-Ni_(3)Se_(2)) presents a high specific capacity of 236 mA h g^(-1)at1 A g^(-1),importantly,it can still possess 117 mA h g^(-1)when the current density is increased up to 100A g^(-1),exhibiting relatively high rate capability.It is much superior to other battery-type TMC cathodes reported in previous studies.Moreover,the cathode also shows the excellent cycling stability with 92%capacity retention after 3,000 cycles.In addition,a hybrid supercapacitor(HSC) is assembled with the obtained Li2-Ni_(3)Se_(2)as the cathode and active carbon(AC) as the anode,which delivers a high energy density of 77 W h kg^(-1)at 4 kW kg^(-1)and long-term durability(90% capacitance retention after 10,000 cycles).Therefore,the strategy not only provides an effective way to realize the controllable vacancy content in TMCs for achieving high-perfo rmance cathodes for HSC,but also further promotes their large-scale applications in the energy storage fields.展开更多
In recent years,manganese-based oxides as an advanced class of cathode materials for zinc-ion batteries(ZIBs)have attracted a great deal of attentions from numerous researchers.However,their slow reaction kinetics,lim...In recent years,manganese-based oxides as an advanced class of cathode materials for zinc-ion batteries(ZIBs)have attracted a great deal of attentions from numerous researchers.However,their slow reaction kinetics,limited active sites and poor electrical conductivity inevitably give rise to the severe performance degradation.To solve these problems,herein,we introduce abundant oxygen vacancies into the flower-likeδ-MnO_(2)nanostructure and effectively modulate the vacancy defects to reach the optimal level(δ-MnO_(2)-x-2.0).The smart design intrinsically tunes the electronic structure,guarantees ion chemisorption-desorption equilibrium and increases the electroactive sites,which not only effectively accelerates charge transfer rate during reaction processes,but also endows more redox reactions,as verified by first-principle calculations.These merits can help the fabricatedδ-MnO_(2)-x-2.0 cathode to present a large specific capacity of 551.8 mAh g^(-1) at 0.5 A g^(-1),high-rate capability of 262.2 mAh g^(-1) at 10 A g^(-1) and an excellent cycle lifespan(83%of capacity retention after 1500 cycles),which is far superior to those of the other metal compound cathodes.In addition,the charge/discharge mechanism of theδ-MnO_(2)-x-2.0 cathode has also been elaborated through ex situ techniques.This work opens up a new pathway for constructing the next-generation high-performance ZIBs cathode materials.展开更多
Short Retraction Notice The paper does not meet the standards of “Chinese Medicine ". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE...Short Retraction Notice The paper does not meet the standards of “Chinese Medicine ". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. The aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. Editor guiding this retraction: Prof. Maythem Saeed (EiC of CM) The full retraction notice in PDF is preceding the original paper, which is marked "RETRACTED".展开更多
Background and Objective:iASPP, an inhibitory member of the apoptosis-stimulating proteins of p53 (ASPP) family, has been found to be up-regulated in various human tumor types.This study was to construct an efficient ...Background and Objective:iASPP, an inhibitory member of the apoptosis-stimulating proteins of p53 (ASPP) family, has been found to be up-regulated in various human tumor types.This study was to construct an efficient doxycycline-regulated, lentiviral vector-mediated knockdown system for iASPP that will allow for inducible down-regulation of iASPP gene expression and preliminary functional analysis.Methods:A pair of complementary oligos with hairpin structures targeting the iASPP gene and a negative control were synthesized, then ligated with pLVTHM vector and sequenced.The fragment containing the shRNA cassette was cloned to pLVCT-tTR-KRAB plasmid.The recombinant vectors were co-transfected with viral packaging mix into 293T cells, and viral supernatant was harvested to determine the titer.After treatment with or without doxycycline, HepG2 cells infected with virus were harvested and the expression of iASPP was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis.Its effects on tumor growth were characterized using MTS assay, soft agar colony formation, and flow cytometry analysis.Results:The lentiviral vector expressing shRNA that targets to the oncogene iASPP was constructed successfully.HepG2 infected with the lentivirus expressing shRNA against iASPP inhibited the expression of iASPP in the presence of doxycycline, which resulted in the repression of tumor cell proliferation and anchorage-independent growth potential.Conclusions:The lentiviral vector-mediated tet-on system demonstrates efficient and inducible knockdown of iASPP in hepatocellular carcinoma cells.iASPP gene may be involved in tumorigenesis and progression of human tumors.展开更多
AIM: To describe the application of complete robotic gastrectomy with transvaginal specimen extraction(TVSE) for gastric cancer patients.METHODS: Between July and November 2014, eight female patients who were diagnose...AIM: To describe the application of complete robotic gastrectomy with transvaginal specimen extraction(TVSE) for gastric cancer patients.METHODS: Between July and November 2014, eight female patients who were diagnosed with gastric adenocarcinoma underwent a TVSE following a full robot-sewn gastrectomy. According to the tumor location, the patients were allocated to two different groups; two patients received robotic total gastrectomy with TVSE and the other six received robotic distal gastrectomy with TVSE.RESULTS: Surgical procedures were successfully performed in all eight cases without conversion. The mean age was 55.3(range, 42-69) years, and the mean body mass index was 23.2(range, 21.6-26.0) kg/m2. The mean total operative time and blood loss were 224(range, 200-298) min and 62.5(range, 50-150) m L, respectively. The mean postoperative hospital stay was 3.6(range, 3-5) d. The mean number of lymph nodes resected was 23.6(range, 17-27). None was readmitted within 30 d of postoperation. During the follow-up, no stricture developed nor was any anastomotic leakage detected.CONCLUSION: It is possible to perform a TVSE following a full robot-sewn gastrectomy with standard D2 lymph node resection for female gastric cancer patients.展开更多
基金supported by the National Major Project of Research and Development,No.2022YFA1105500(to SZ)the National Natural Science Foundation of China,No.81870975(to SZ)Innovation Program for Graduate Students in Jiangsu Province of China,No.KYCX223335(to MZ)。
文摘CD36 is a highly glycosylated integral membrane protein that belongs to the scavenger receptor class B family and regulates the pathological progress of metabolic diseases.CD36 was recently found to be widely expressed in various cell types in the nervous system,including endothelial cells,pericytes,astrocytes,and microglia.CD36 mediates a number of regulatory processes,such as endothelial dysfunction,oxidative stress,mitochondrial dysfunction,and inflammatory responses,which are involved in many central nervous system diseases,such as stroke,Alzheimer’s disease,Parkinson’s disease,and spinal cord injury.CD36 antagonists can suppress CD36 expression or prevent CD36 binding to its ligand,thereby achieving inhibition of CD36-mediated pathways or functions.Here,we reviewed the mechanisms of action of CD36 antagonists,such as Salvianolic acid B,tanshinone IIA,curcumin,sulfosuccinimidyl oleate,antioxidants,and small-molecule compounds.Moreover,we predicted the structures of binding sites between CD36 and antagonists.These sites can provide targets for more efficient and safer CD36 antagonists for the treatment of central nervous system diseases.
基金The research has been generously supported by Tianjin Education Commission Scientific Research Program(2020KJ056),ChinaTianjin Science and Technology Planning Project(22YDTPJC00970),China.The authors would like to express their sincere appreciation for all support provided.
文摘A real-time adaptive roles allocation method based on reinforcement learning is proposed to improve humanrobot cooperation performance for a curtain wall installation task.This method breaks the traditional idea that the robot is regarded as the follower or only adjusts the leader and the follower in cooperation.In this paper,a self-learning method is proposed which can dynamically adapt and continuously adjust the initiative weight of the robot according to the change of the task.Firstly,the physical human-robot cooperation model,including the role factor is built.Then,a reinforcement learningmodel that can adjust the role factor in real time is established,and a reward and actionmodel is designed.The role factor can be adjusted continuously according to the comprehensive performance of the human-robot interaction force and the robot’s Jerk during the repeated installation.Finally,the roles adjustment rule established above continuously improves the comprehensive performance.Experiments of the dynamic roles allocation and the effect of the performance weighting coefficient on the result have been verified.The results show that the proposed method can realize the role adaptation and achieve the dual optimization goal of reducing the sum of the cooperator force and the robot’s Jerk.
基金supported by DFGP Project of Fauna of Guangdong-202115, Nanling National Park Construction Project (ZD22-ZC016AG)the National Animal Collection Resource Center,China。
文摘The subfamily Megophryinae,as a representative batrachian group of the Oriental Realm and one of the most diverse groups of amphibians,has attracted considerable attention due to continued conjecture regarding its generic classification and failure to reach a satisfactory consensus.China boasts the richest diversity of Asian horned toads,containing some two thirds of the total species cataloged.However,most species have a complicated taxonomic history,resulting in multiple misidentifications.As such,an overall clarification of historical records and regional checklists is required.In the current investigation,we established the phylogeny of the Asian horned toads and performed detailed examinations with redefinitions of several important morphological traits.Based on the phylogenetic relationships and morphological differences,we propose a new ten-genus classification for the Asian horned toad subfamily Megophryinae:i.e.,Brachytarsophrys,Atympanophrys,Grillitschia,Sarawakiphrys gen.nov.,Jingophrys gen.nov.,Xenophrys,Megophrys,Pelobatrachus,Ophryophryne,and Boulenophrys.Revisions on the diagnosability,distribution,and content of each genus are provided.Furthermore,we present a careful review of the taxonomic history of Asian horned toad species from China and provide a monograph of congeners,including six species of Brachytarsophrys,four species of Atympanophrys,five species of Jingophrys gen.nov.,10 species of Xenophrys,two species of Ophryophryne,and 60 species of Boulenophrys.Finally,we discuss the importance of traditional morphological traits based on multiple populations in taxonomic work as well as taxonomic inflation caused by the genetic species delimitation.
基金funded by the Natural Sciences and Engineering Research Council of Canada(NSERC RGPIN-2017-05537).
文摘The unconfined compressive strength(UCS)of alkali-activated slag(AAS)-based cemented paste backfill(CPB)is influenced by multiple design parameters.However,the experimental methods are limited to understanding the relationships between a single design parameter and the UCS,independently of each other.Although machine learning(ML)methods have proven efficient in understanding relationships between multiple parameters and the UCS of ordinary Portland cement(OPC)-based CPB,there is a lack of ML research on AAS-based CPB.In this study,two ensemble ML methods,comprising gradient boosting regression(GBR)and random forest(RF),were built on a dataset collected from literature alongside two other single ML methods,support vector regression(SVR)and artificial neural network(ANN).The results revealed that the ensemble learning methods outperformed the single learning methods in predicting the UCS of AAS-based CPB.Relative importance analysis based on the bestperforming model(GBR)indicated that curing time and water-to-binder ratio were the most critical input parameters in the model.Finally,the GBR model with the highest accuracy was proposed for the UCS predictions of AAS-based CPB.
基金supported by the National Natural Science Foundation of China,Nos.32060212(to YW),81760648(to XWY),81560118(to BYZ)Project of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation,Nos.202101AY070001-006(to XWY)and 2018FE001(-161)(to JS)+2 种基金Yunnan Applied Basic Research Project Foundation,No.2019FB128(to YW)Project of Yunnan Province Clinical Research Centerfor Chronic Kidney Disease,No.202102AA10060(to BYZ)a grant from Scientific Research Foundation of Department of Education of Yunnan Province,No.2021J0205(to SSL)。
文摘The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote structural and functional recovery after spinal cord injury.Previous studies have shown that peptides can promote substantial repair and regeneration of injured tissue.While amphibians have a pronounced ability to regenerate the spinal cord,few studies have investigated the effect of amphibian spinal cord-derived peptides on spinal cord injury.Here we report for the first time the successful identification and isolation of a new polypeptide,VD11(amino acid sequence:VDELWPPWLPC),from the spinal cord of an endemic Chinese amphibian(Odorrana schmackeri).In vitro experiments showed that VD11 promoted the secretion of nerve growth factor and brain-derived neurotrophic factor in BV2 cells stimulated with lipopolysaccharide,as well as the proliferation and synaptic elongation of PC12 cells subjected to hypoxia.In vivo experiments showed that intravertebral injection of VD11 markedly promoted recovery of motor function in rats with spinal cord injury,alleviated pathological damage,and promoted axonal regeneration.Furthermore,RNA sequencing and western blotting showed that VD11 may affect spinal cord injury through activation of the AMPK and AKT signaling pathways.In summary,we discovered a novel amphibian-derived peptide that promotes structural and functional recovery after spinal cord injury.
文摘An improved rheo-optic in situ synchronous measurement system was employed to investigate the gelation behaviour and mechanism of waxy crude oil emulsions. By combining transmitted natural light and reflected polarized light microscopy, a multiangle composite light source was built to achieve the simultaneous observation of wax crystals and emulsified water droplets, as well as their dynamic aggregation process. Main outcomes on the microscopic mechanism were obtained by developed microscopic image processing method. It was found that the microstructure of W/O waxy crude oil emulsion has the evolution of “individual structure--homogeneous aggregate structure--heterogeneous coaggregate structure--floc structure” during the static cooling, which results in the four stages during gelation process. Different from previous studies, the aggregation of emulsified water droplets was found to be more significant and contributes to the formation and development of the wax crystals-emulsified water droplets coaggregate, which plays a decisive role in the further evolution of the gelled microstructure. Time series microscopic images show the dynamic aggregation of emulsified water droplets and wax crystals. Two different aggregation behaviours between wax crystals and water droplets were observed. That wax crystals can not only embed in gaps between adjacent water droplets and enhance the structure, but also surround the outside of the water droplets and continue to grow resulting in the interconnection of different coaggregates to form a larger floc structure. In addition, correlation between viscoelasticity and microstructure evolution of waxy crude oil emulsions of different water contents was discussed. With increasing water contents, the microstructure is changed from wax crystal flocculation structure as the main skeleton and the emulsified water droplets embedded in it, into the aggregation of emulsified water droplets occupying the main position. When the number of wax crystals and water droplets reaches a certain ratio, did wax crystals form coaggregates with emulsified water droplets, and the remaining wax crystals formed an overall flocculation structure, the viscoelasticity of the waxy crude oil emulsion is the highest.
基金supported by the National Natural Science Foundation of China(Grant No.51672144,51572137,51702181,52072196,52002199,52002200)the Major Basic Research Program of Natural Science Foundation of Shandong Province(Grant No.ZR2020ZD09)+6 种基金the Shandong Provincial Key Research and Development Program(SPKR&DP)(Grant No.2019GGX102055)the Natural Science Foundation of Shandong Province(Grant No.ZR2019BEM042,ZR2020QE063)the Innovation and Technology Program of Shandong Province(Grant No.2020KJA004)the Guangdong Basic and Applied Basic Research Foundation(Grant No.2019A1515110933)the China Postdoctoral Science Foundation(Grant No.2020M683450)the Taishan Scholars Program of Shandong Province(No.ts201511034)the Postdoctoral Innovation Project of Shandong Province(Grant no.202101020)。
文摘The poor rate capability and low capacity are huge barriers to realize the commercial applications of battery-type transition metal compounds(TMCs) cathode.Herein,numerous Se vacancy defects are introduced into the Ni_(3)Se_(2)lamellas by pre-lithiation technique,which can be acted as a novel class of battery-type cathode for hybrid supercapacitors.Appropriately modulating the contents of the preembedded lithium(Li) ions can induce a controllable vacancy content in the series of as-prepared products,effectively endowing a fast reaction kinetic and high activity for the cathode.Benefiting from the distinct design,the optimized cathode(Li2-Ni_(3)Se_(2)) presents a high specific capacity of 236 mA h g^(-1)at1 A g^(-1),importantly,it can still possess 117 mA h g^(-1)when the current density is increased up to 100A g^(-1),exhibiting relatively high rate capability.It is much superior to other battery-type TMC cathodes reported in previous studies.Moreover,the cathode also shows the excellent cycling stability with 92%capacity retention after 3,000 cycles.In addition,a hybrid supercapacitor(HSC) is assembled with the obtained Li2-Ni_(3)Se_(2)as the cathode and active carbon(AC) as the anode,which delivers a high energy density of 77 W h kg^(-1)at 4 kW kg^(-1)and long-term durability(90% capacitance retention after 10,000 cycles).Therefore,the strategy not only provides an effective way to realize the controllable vacancy content in TMCs for achieving high-perfo rmance cathodes for HSC,but also further promotes their large-scale applications in the energy storage fields.
基金supported by the National Natural Science Foundation of China under Grant Nos. 52072196, 52002200, 52102106 and 52002199Major Basic Research Program of the Natural Science Foundation of Shandong Province under Grant No. ZR2020ZD09+2 种基金the Natural Science Foundation of Shandong Province under Grant No. ZR2020QE063the Innovation and Technology Program of Shandong Province under Grant No. 2020KJA004the Taishan Scholars Program of Shandong Province under Grant No. ts201511034
文摘In recent years,manganese-based oxides as an advanced class of cathode materials for zinc-ion batteries(ZIBs)have attracted a great deal of attentions from numerous researchers.However,their slow reaction kinetics,limited active sites and poor electrical conductivity inevitably give rise to the severe performance degradation.To solve these problems,herein,we introduce abundant oxygen vacancies into the flower-likeδ-MnO_(2)nanostructure and effectively modulate the vacancy defects to reach the optimal level(δ-MnO_(2)-x-2.0).The smart design intrinsically tunes the electronic structure,guarantees ion chemisorption-desorption equilibrium and increases the electroactive sites,which not only effectively accelerates charge transfer rate during reaction processes,but also endows more redox reactions,as verified by first-principle calculations.These merits can help the fabricatedδ-MnO_(2)-x-2.0 cathode to present a large specific capacity of 551.8 mAh g^(-1) at 0.5 A g^(-1),high-rate capability of 262.2 mAh g^(-1) at 10 A g^(-1) and an excellent cycle lifespan(83%of capacity retention after 1500 cycles),which is far superior to those of the other metal compound cathodes.In addition,the charge/discharge mechanism of theδ-MnO_(2)-x-2.0 cathode has also been elaborated through ex situ techniques.This work opens up a new pathway for constructing the next-generation high-performance ZIBs cathode materials.
文摘Short Retraction Notice The paper does not meet the standards of “Chinese Medicine ". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. The aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. Editor guiding this retraction: Prof. Maythem Saeed (EiC of CM) The full retraction notice in PDF is preceding the original paper, which is marked "RETRACTED".
基金Supplementary information is linked to the online version of the paper on the Cell Research website.Acknowledgments We thank Dr David Westaway (University of Alberta) for TgCRND8 mice, Dr David Baltimore (California Institute of Technology) for lentiviral constructs, Dr Raphael Kopan (Washington University) for the plasmid of myc-tagged NotchAE and Dr Johan Lundkvist (Karolinska Institutet) for the plasmid of Gal4-driven luciferase reporter gene, the plasmid of APP/CTFI3-GVP and NAE-GVP. We appreciate Shunmei Xin, Shan Chen and Xianglu Zeng for their technical assistance. We thank all members of the lab for sharing reagents and advice. This research was supported by the Ministry of Science and Technology (2009ZX09103-684), the National Natural Science Foundation of China (30621091, 30625014, 30623003, 30871285 and 90713047), the Shanghai Municipal Commission for Science and Technology (07PJ14099 and 09JC1416400), and the Chinese Academy of Sciences (2007KIP204).
文摘Background and Objective:iASPP, an inhibitory member of the apoptosis-stimulating proteins of p53 (ASPP) family, has been found to be up-regulated in various human tumor types.This study was to construct an efficient doxycycline-regulated, lentiviral vector-mediated knockdown system for iASPP that will allow for inducible down-regulation of iASPP gene expression and preliminary functional analysis.Methods:A pair of complementary oligos with hairpin structures targeting the iASPP gene and a negative control were synthesized, then ligated with pLVTHM vector and sequenced.The fragment containing the shRNA cassette was cloned to pLVCT-tTR-KRAB plasmid.The recombinant vectors were co-transfected with viral packaging mix into 293T cells, and viral supernatant was harvested to determine the titer.After treatment with or without doxycycline, HepG2 cells infected with virus were harvested and the expression of iASPP was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis.Its effects on tumor growth were characterized using MTS assay, soft agar colony formation, and flow cytometry analysis.Results:The lentiviral vector expressing shRNA that targets to the oncogene iASPP was constructed successfully.HepG2 infected with the lentivirus expressing shRNA against iASPP inhibited the expression of iASPP in the presence of doxycycline, which resulted in the repression of tumor cell proliferation and anchorage-independent growth potential.Conclusions:The lentiviral vector-mediated tet-on system demonstrates efficient and inducible knockdown of iASPP in hepatocellular carcinoma cells.iASPP gene may be involved in tumorigenesis and progression of human tumors.
文摘AIM: To describe the application of complete robotic gastrectomy with transvaginal specimen extraction(TVSE) for gastric cancer patients.METHODS: Between July and November 2014, eight female patients who were diagnosed with gastric adenocarcinoma underwent a TVSE following a full robot-sewn gastrectomy. According to the tumor location, the patients were allocated to two different groups; two patients received robotic total gastrectomy with TVSE and the other six received robotic distal gastrectomy with TVSE.RESULTS: Surgical procedures were successfully performed in all eight cases without conversion. The mean age was 55.3(range, 42-69) years, and the mean body mass index was 23.2(range, 21.6-26.0) kg/m2. The mean total operative time and blood loss were 224(range, 200-298) min and 62.5(range, 50-150) m L, respectively. The mean postoperative hospital stay was 3.6(range, 3-5) d. The mean number of lymph nodes resected was 23.6(range, 17-27). None was readmitted within 30 d of postoperation. During the follow-up, no stricture developed nor was any anastomotic leakage detected.CONCLUSION: It is possible to perform a TVSE following a full robot-sewn gastrectomy with standard D2 lymph node resection for female gastric cancer patients.