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Stratigraphy of the Triassic-Jurassic Boundary Successions of the Southern Margin of the Junggar Basin,Northwestern China 被引量:7
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作者 SHA Jingeng Vivi VAJDA +9 位作者 PAN Yanhong Linda LARSSON YAO Xiaogang ZHANG Xiaolin WANG Yaqiong CHENG Xiansheng jiang baoyu DENG Shenghui CHEN Siwei PENG Bo 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2011年第2期421-436,共16页
The Triassic-Jurassic (Tr-J) boundary marks a major extinction event, which (~200 Ma)resulted in global extinctions of fauna and flora both in the marine and terrestrial realms. There prevail great challenges in ... The Triassic-Jurassic (Tr-J) boundary marks a major extinction event, which (~200 Ma)resulted in global extinctions of fauna and flora both in the marine and terrestrial realms. There prevail great challenges in determining the exact location of the terrestrial Tr-J boundary, because of endemism of taxa and the scarcity of fossils in terrestrial settings leading to difficulties in linking marine and terrestrial sedimentary successions. Investigation based on palynology and bivalves has been carried out over a 1113 m thick section, which is subdivided into 132 beds, along the Haojiagou valley on the southern margin of the Junggar Basin of the northern Xinjiang, northwestern China. The terrestrial Lower Jurassic is conformably resting on the Upper Triassic strata. The Upper Triassic covers the Huangshanjie Formation overlaid by the Haojiagou Formation, while the Lower Jurassic comprises the Badaowan Formation followed by the Sangonghe Formation. Fifty six pollen and spore taxa and one algal taxon were identified from the sediments. Based on the key-species and abundance of spores and pollen, three zones were erected: the Late Triassic (Rhaetian) Aratrisporites-Alisporites Assemblage, the Early Jurassic (Hettangian) Perinopollenites-Pinuspollenites Assemblage, and the Sinemurian Perinopollenites-Cycadopites Assemblage. The Tr-J boundary is placed between bed 44and 45 coincident with the boundary between the Haojiagou and Badaowan formations. Beds with Ferganoconcha (?), Unio-Ferganoconcha and Waagenoperna-Yananoconcha bivalve assemblages are recognized. The Ferganoconcha (?) bed is limited to the upper Haojiagou Formation,Unio- Ferganoconcha and Waagenoperna- Yananoconcha assemblages are present in the middle and upper members of the Badaowan Formation. The sedimentary succession is interpreted as terrestrial with two mainly lake deposit intervals within Haojiagou and Badaowan formations, yielding fresh water algae and bivalves. However, the presence of brackish water algae Tasmanites and the marine-littoral facies bivalve Waagenoperna from the Badaowan Formation indicate that the Junggar Basin was influenced by sea water caused by transgressions from the northern Tethys, during the Sinemurian. 展开更多
关键词 Biostratigrapy Triassic-Jurassic Hettangian-Sinemurian PALYNOLOGY BIVALVE Haojiagou of Junggar Basin northwestern China
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结核分枝杆菌Rv2626c蛋白的原核表达、纯化及抗原活性鉴定
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作者 张光磊 孙田华 +7 位作者 吴智远 张婷婷 胡丽娜 王婷 李会 蒋保余 李朋伟 焦磊 《中国热带医学》 CAS 北大核心 2024年第4期472-477,共6页
目的构建结核分枝杆菌Rv2626c蛋白的重组表达载体,在大肠埃希菌(Escherichia coli,E.coli)中表达、纯化后研究Rv2626c重组蛋白的抗原性。方法将GenBank公布的结核分枝杆菌H37Rv株Rv2626c蛋白的氨基酸序列(登录号:CCP45424.1)按E.coli密... 目的构建结核分枝杆菌Rv2626c蛋白的重组表达载体,在大肠埃希菌(Escherichia coli,E.coli)中表达、纯化后研究Rv2626c重组蛋白的抗原性。方法将GenBank公布的结核分枝杆菌H37Rv株Rv2626c蛋白的氨基酸序列(登录号:CCP45424.1)按E.coli密码子偏好转换为对应的DNA序列,合成该DNA序列并克隆至pET24a(+)质粒上,构建pET24a(+)-Rv2626c重组质粒,转化至E.coli BL21(DE3)中,以不同的异丙基-β-D-硫代半乳糖苷(isopropylβ-D-thiogalactopyranoside,IPTG)浓度、温度和时间条件下诱导表达Rv2626c蛋白,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)和蛋白免疫印迹法(Western Blot)对Rv2626c重组蛋白进行鉴定。利用镍螯合亲和层析法纯化Rv2626c重组蛋白并将其免疫青紫蓝兔,制备抗Rv2626c抗血清,采用Western Blot和酶联免疫吸附法分别检测抗血清的特异性和效价。结果pET24a(+)-Rv2626c重组质粒构建成功。SDS-PAGE检测显示:转入该重组质粒的E.coli经IPTG诱导后表达Rv2626c重组蛋白,分子量约14500,大小与预期相符;Rv2626c重组蛋白的最佳诱导表达条件为31℃1.0 mmol/L IPTG诱导表达6 h,目的蛋白主要以可溶性形式存在,与Western Blot检测结果一致。Rv2626c重组蛋白免疫青紫蓝兔获得的高免血清特异性良好,经酶联免疫吸附法测定该血清效价为1∶256000。结论在E.coli中成功表达结核分枝杆菌Rv2626c蛋白,且纯化的Rv2626c重组蛋白具有较好的纯度和抗原活性,为进一步揭示其生物学功能奠定基础。 展开更多
关键词 结核分枝杆菌 Rv2626c重组蛋白 抗原活性鉴定
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公众药品不良反应认知和行为意向的调查分析 被引量:3
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作者 潘代勇 蒋宝玉 黄秀云 《药物不良反应杂志》 CSCD 2020年第4期252-256,共5页
目的了解公众对药品不良反应(ADR)的认知和行为意向情况。方法采用自行设计的纸质问卷和微信问卷2种形式,对2018年9月至12月在广西科技大学第一附属医院门诊候诊及等候取药的患者或家属进行现场问卷调查。被调查者以无记名方式填写问卷... 目的了解公众对药品不良反应(ADR)的认知和行为意向情况。方法采用自行设计的纸质问卷和微信问卷2种形式,对2018年9月至12月在广西科技大学第一附属医院门诊候诊及等候取药的患者或家属进行现场问卷调查。被调查者以无记名方式填写问卷,答毕现场回收。经2位药师筛选合格问卷,对调查问卷中关于ADR认知和行为意向的每个问题选项赋予一定分值,对得分进行百分化,并对调查问卷进行统计分析。结果共回收问卷519份,合格问卷508份,有效回收率为97.9%。受访者对ADR认知的中位得分为50(0,100),行为意向中位得分为86(36,100)。≥60岁的老年人对ADR认知和行为意向的得分均低于20~39岁和40~59岁人群(均P<0.05),大学/大学专科学历者对ADR认知和行为意向得分明显高于中学/中等专业学历者(均P<0.05);城镇居民对ADR认知和行为意向得分均明显高于郊区及农村居民(均P<0.05)。仅有55.9%(284/508)的调查对象对ADR定义的选择是正确的,44.1%(224/508)调查对象认为ADR是用药错误、药品质量或医疗差错造成的,62.6%(318/508)认为发生ADR应该由医院、药品生产企业和监管部门承担责任。在发生ADR后,有91.9%(467/508)的调查对象表示愿意向相关部门报告,但有25.6%(130/508)的调查对象不知道报告部门和不知道如何填写报表。结论公众对ADR的认知度较低;尽管有报告ADR的意向,但因对ADR的认知度较低,对报告部门、报告途径等不了解而受限。 展开更多
关键词 药物相关副作用和不良反应 药物不良反应报告系统 患者参与 问卷调查
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