This study was aimed to isolate ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) from tea plant [Camellia sinensis (L.) O. Kuntze]. In the study of transcriptional profiling of gene expression ...This study was aimed to isolate ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) from tea plant [Camellia sinensis (L.) O. Kuntze]. In the study of transcriptional profiling of gene expression from tea flower bud development stage by cDNA-AFLP (cDNA amplified fragment length polymorphism), we have isolated some transcript-derived fragments (TDFs) occurring in both the young and mature flower bud. One of them showed a high degree of similarity to RbcS. Based on the fragment, the full length of RbcS with 769-bp (EF011075) cDNA was obtained via rapid amplification of cDNA ends (RACE). It contained an open reading frame of 176 amino acids consisting of a chloroplast transit peptide with 52 amino acids and a mature protein of 124 amino acids. The amino acids sequence presented a high identity to those of other plant RbcS genes. It also contains three conserved domains and a protein kinase C phosphorylation site, one tyrosine kinase phosphorylation site and two N-myristoylation sites. Analysis by RT-PCR showed that the expression of RbcS in tea from high to low was leaf, young stem, young flower bud and mature flower bud, respectively. The isolation of the tea Rubisco small subunit gene establishes a good foundation for further study on the photosynthesis of tea plant.展开更多
基金supported by the National Natural Science Foundation of China (30871568)National Key Technology R&D Program of China(2008BAC0B03).
文摘This study was aimed to isolate ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) from tea plant [Camellia sinensis (L.) O. Kuntze]. In the study of transcriptional profiling of gene expression from tea flower bud development stage by cDNA-AFLP (cDNA amplified fragment length polymorphism), we have isolated some transcript-derived fragments (TDFs) occurring in both the young and mature flower bud. One of them showed a high degree of similarity to RbcS. Based on the fragment, the full length of RbcS with 769-bp (EF011075) cDNA was obtained via rapid amplification of cDNA ends (RACE). It contained an open reading frame of 176 amino acids consisting of a chloroplast transit peptide with 52 amino acids and a mature protein of 124 amino acids. The amino acids sequence presented a high identity to those of other plant RbcS genes. It also contains three conserved domains and a protein kinase C phosphorylation site, one tyrosine kinase phosphorylation site and two N-myristoylation sites. Analysis by RT-PCR showed that the expression of RbcS in tea from high to low was leaf, young stem, young flower bud and mature flower bud, respectively. The isolation of the tea Rubisco small subunit gene establishes a good foundation for further study on the photosynthesis of tea plant.
基金supported by the National Natural Science Foundation of China(No.31900846)the Project of Institute of Sports Medicine and Health of Chengdu Sport University/Institute of Zheng Huaixian Orthopaedics and Traumatology(No.CX21B04)the Project from the Key Laboratory of Sports Medicine of General Administration of Sport of China and Sichuan Key Laboratory of Sports Medicine(No.2022-A016)。
文摘非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)是代谢综合征的主要疾病之一。随着NAFLD在全球范围内的日益流行,NAFLD的防治面临巨大的挑战。近些年,国内外学者针对NAFLD开展了大量研究,但其发病机制仍不清楚。内质网应激(endoplasmic reticulum stress,ERS)是由未折叠或错误折叠蛋白的积累所引起,作为对ERS的反应,细胞通过启动未折叠蛋白反应(unfolded protein response,UPR)的保护机制来帮助恢复正常的内质网功能。肝细胞脂质异常积聚、炎症反应加剧、肝细胞凋亡增加和胰岛素抵抗(insulin resistance,IR)是NAFLD的主要发病因素和特征,与肝脏ERS密切相关。大量研究表明,运动作为一种低成本治疗手段,可有效预防和改善NAFLD,其机制与运动调节ERS水平相关。本文从ERS的角度,综述运动改善NAFLD的作用机制研究进展。运动改善NAFLD的机制与运动通过调节ERS来调控肝细胞脂质代谢、减轻炎症反应、减少肝细胞凋亡和改善IR有关。