A novel two-dimensional nanosheet fluorescent probe embedded with rare earth Eu(Ⅲ)was developed to detect dipicolinic acid(DPA),a biomarker of Bacillus anthracis bacterial spores.DPA,a major component of Bacillus ant...A novel two-dimensional nanosheet fluorescent probe embedded with rare earth Eu(Ⅲ)was developed to detect dipicolinic acid(DPA),a biomarker of Bacillus anthracis bacterial spores.DPA,a major component of Bacillus anthracis spores which were highly toxic to humans,was not found in other common bacteria.The ability to detect ultra-low concentrations of DPA would therefore be of great significance.Eu(Ⅲ)-doped ytterbium hydroxide nanosheets were obtained by mechanical exfoliation from layered rare-earth hydroxide(LRH)materials.The crystallinities,layered structure and morphology of the as-synthesized nanosheets were studied by power X-ray diffraction,transmission electron microscopy and atomic force microscopy.Eu(Ⅲ)emission increased linearly with DPA addition in the range of 0.1~30μmol/L.Based on the antenna effect,the detection limit of DPA was 0.078μmol/L and much lower than the infective dose of Bacillus anthracis in humans of 60μmol/L.The nanosheet fluorescent probe exhibited good specificity toward DPA,and the interferences with selected aromatic ligands and amino acids were observed to be negligibly small in comparison with that of DPA.Our findings provide a basis for the application of Eu(Ⅲ)-doped nanosheets for accurate,sensitive,and selective monitoring of DPA as a biomarker of anthrax.展开更多
基金the Department of Science and Technology of Hebei Province(No.17272610)。
文摘A novel two-dimensional nanosheet fluorescent probe embedded with rare earth Eu(Ⅲ)was developed to detect dipicolinic acid(DPA),a biomarker of Bacillus anthracis bacterial spores.DPA,a major component of Bacillus anthracis spores which were highly toxic to humans,was not found in other common bacteria.The ability to detect ultra-low concentrations of DPA would therefore be of great significance.Eu(Ⅲ)-doped ytterbium hydroxide nanosheets were obtained by mechanical exfoliation from layered rare-earth hydroxide(LRH)materials.The crystallinities,layered structure and morphology of the as-synthesized nanosheets were studied by power X-ray diffraction,transmission electron microscopy and atomic force microscopy.Eu(Ⅲ)emission increased linearly with DPA addition in the range of 0.1~30μmol/L.Based on the antenna effect,the detection limit of DPA was 0.078μmol/L and much lower than the infective dose of Bacillus anthracis in humans of 60μmol/L.The nanosheet fluorescent probe exhibited good specificity toward DPA,and the interferences with selected aromatic ligands and amino acids were observed to be negligibly small in comparison with that of DPA.Our findings provide a basis for the application of Eu(Ⅲ)-doped nanosheets for accurate,sensitive,and selective monitoring of DPA as a biomarker of anthrax.
