血液和尿液中锂含量的电感耦合等离子体-质谱测定法

目的建立血液和尿液中锂含量的电感耦合等离子体-质谱测定法。方法利用1%硝酸、0.1%曲拉通X-100-1%硝酸溶液分别对尿液和血液样品进行12.5、20倍直接稀释后上机检测,以钪(Sc)作为内标元素,于标准模式下完成检测。结果该方法利用标准曲线完成生物样品中锂含量测定,线性范围为0.03~50.0μg/L,相关系数为0.9999,方法的检出限为0.008μg/L,样品加标回收率为86.1%~103.8%,精密度为0.8%~4.2%,质量控制样品测定结果满意。结论本研究建立了一种简便快捷的测定生物样品中锂含量的方法,并对实验中各影响参数进行优化研究,详细探讨了实验过程中各参数变化对于实验结果的影响。方法前处理简便快捷、灵敏度高、稳定性好、检出限低,适用范围广,适合于各类人群生物样品锂含量的测定。

Relationship between occupational stress and salivary sIgA and lysozyme in assembly line workers

Anumber of studies have investigated the effects of stress on mental health and cardiovascular diseases and the relationship between stress and various components of the immune system, and they found that chronic stress is associated with suppression of a variety of immune parameters. However, acute stress is often associated with transient increases in immune activationl A few studies have also examined the slgA response to occupational stress. Zeier et al2 measured salivary slgA in air traffic controllers before and immediately after radar working sessions lasting approximately 100 minutes. Counter to the author＇s expectation, slgA was significantly elevated after the stressful work-session. Ng et al3 found slgA correlated inversely with self-reported levels of stress among nurses in Singapore. In the United States and Australia, studies on occupational stress in the nursing profession using salivary IgA as a stress indicator have also been conducted. Both studies indicated that the nurses＇ stress was significantly associated with a decreased salivary IgA secretion rates.4.5 A study has indicated salivary lysozyme as a promising marker of the effects of stress on nonspecific immunity, demonstrating a negative relation between perceived stress, as indicated by a stress score obtained by means of a questionnaire, and actual stress in the form of an undergraduate examination and salivary lysozyme levels.6 Ng et al3 have not found a relationship between salivary lysozyme levels and self-reported levels of stress among nurses in Singapore.

微波消解-石墨炉原子吸收法测定微量人发中铟

目的建立人发样品中铟的石墨炉原子吸收测定方法。方法样品经微波消解后,去离子水稀释定容,以胶体钯为基体改进剂,石墨炉原子吸收光谱仪进行检测,外标法定量。结果方法的线性范围为5.0~50.00μg/L,相关系数(r)=0.9996,方法的检测限为0.26μg/g;精密度为1.63%~2.12%;加标回收率为97.6%~100.5%。结论该方法样品和消解液用量小,操作简单,效率高,目标物损失少,技术指标满足《职业卫生标准制定指南第5部分:生物材料中化学物质测定方法》的要求。

电渣重熔工人接触氟的时间加权平均浓度与尿氟含量及骨密度减少的关联分析

目的探讨电渣重熔车间工人接触的工作场所空气中氟的时间加权平均浓度(TWA)与尿氟含量及骨密度的关联。方法测定电渣重熔车间工人接触的工作场所空气中氟化物的TWA,同时进行尿氟测定、骨片和骨密度检查,并对结果进行统计分析。结果氟化物8 h TWA的均值:精炼组0.96 mg/m3,配电维修组0.47 mg/m3,管理辅助组0.37 mg/m3;高年龄组工人尿氟浓度明显高于低年龄组,精炼组尿氟浓度明显高于配电维修组;骨片结果未见明显异常,尿氟含量在2.0 mg/g Cr以上发生骨质减少的比率明显增高。结论接触空气氟的TWA与尿氟浓度存在一定的剂量-效应关系,氟化物工作场所职业接触限值与作业工人生物接触限值的匹配性较好,骨密度异常的比率在某种程度上与尿氟浓度具有相关性、一致性。