CD137信号通过有氧糖酵解途径促进小鼠肺动脉内皮细胞增殖

目的:探讨CD137信号是否通过调控有氧糖酵解途径促进小鼠肺动脉内皮细胞(PAECs)增殖。方法:将小鼠PAECs分组如下:(1)空白对照组、同型对照组、刺激因子TNF-α(10μg/L)组、ET-1(10 mmol/L)和5-HT(1μmol/L)组,各组刺激细胞24 h;(2)选择TNF-α刺激细胞24 h后分为对照组、CD137激动剂组(CD137L重组蛋白5 mg/L,激动CD137-CD137L信号)、c-Myc抑制剂组(c-Myc抑制剂10074-G5 10μmol/L,DMSO溶解,预处理细胞30 min,再加入CD137L重组蛋白)和DMSO组(加入与c-Myc抑制剂组等量DMSO预处理细胞30 min,再予CD137L重组蛋白刺激);(3)TNF-α刺激细胞24 h后分为对照组、CD137激动剂组和2-脱氧葡萄糖(2-DG)组(10 mmol/L糖酵解抑制剂2-DG预处理细胞30 min,再加入CD137L重组蛋白)。分别采用流式细胞术及Western blot检测细胞CD137膜蛋白及总蛋白表达。Western blot及酶学分析分别检测细胞己糖激酶(HK2)、6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶3(PFKFB3)和c-Myc蛋白表达及酶活性。葡萄糖氧化酶法及乳酸脱氢酶比色法检测细胞葡萄糖摄取量及乳酸水平。CCK-8法和EdU法检测细胞增殖。结果:与对照组相比,刺激因子TNF-α、ET-1及5-HT刺激24 h可明显增加PAECs的CD137膜蛋白及总蛋白的表达(P<0.05)。CD137激动剂组细胞HK2及PFKFB3蛋白表达及酶活性显著高于对照组;CD137激动剂组细胞乳酸水平高于对照组,葡萄糖消耗较对照组多(P<0.05)。激活CD137信号后PAECs的c-Myc蛋白表达高于对照组,而应用c-Myc抑制剂10074-G5可显著抑制CD137信号的促糖酵解作用(P<0.05)。CCK-8及EdU增殖试验显示激活CD137信号可显著促进PAECs增殖,有氧糖酵解抑制剂2-DG可抑制其促细胞增殖作用(P<0.05)。结论:CD137信号可能通过上调c-Myc增强小鼠PAECs有氧糖酵解,进而促进细胞增殖。

心理干预对晚期卵巢癌化疗患者的疗效观察

目的探讨心理干预对晚期卵巢癌患者的疗效。方法选取80例晚期卵巢癌患者实施西他赛联合卡铂或紫杉醇联合顺铂治疗，按照护理方式对患者实施分组，对照组患者为常规护理，观察组患者则在对照组基础上实施心理护理。观察并比较2组患者临床干预的效果。结果观察组治疗有效率和护理满意率，均明显高于对照组；实施分组护理后，观察组患者的心理状况得到明显改善，治疗后心理状况比较观察组患者较优，且和对照组存在明显差异。结论对晚期卵巢癌化疗患者，在常规护理的基础上加用心里干预，可显著提高治疗有效率，改善患者的心理状况。

Transmission spectra of one-di mensional photonic crystals including negative-refractive-index media

We introduce a new model of one-dimensional （1D） photonic crystal composed of alternately arranged RHM and LHM layers with positive and negative refractive indices respectively, for which the transmission spectra of the model are calculated numerically with the transfer matrix method, and the bandgap structure and the polarization properties are analyzed. We found that the introduction of negative refractive index layers （i.e. LHM medium layers） gives rise to some peculiar band-gap structure and polarization properties as follows. Firstly, the forbidden bands are extremely wide and the transmission bands are very sharp without oscillation;and secondly, the change of incident angle has different influences on the forbidden bands of TE and TM modes. For the TM mode, the forbidden band width decreases substantially and finally vanishes, and for the TE mode with central wavelength, the total reflection happens at anv incident anale.

外阴癌术后调强适形放疗15例临床观察

目的观察调强适形放射治疗在外阴癌术后患者中放疗的疗效及毒副反应。方法对外阴癌术后病理提示腹股沟淋巴结转移的患者,行术后放化疗同步治疗,放疗技术采用调强适形放疗,对术后复查仍存在腹股沟或盆腔淋巴结肿大的患者采用淋巴结局部同步加量。放疗靶区包括:GTVnd为术后体检或相关影像学检查存在腹股沟或盆腔淋巴结肿大;CTV为双侧腹股沟、髂外及髂内淋巴结引流区和(或)外阴局部。靶区的总剂量及分割剂量分别为:PGTVnd 59.8~62.1 Gy/26f,2.3~2.4 Gy/f;PTV 46.8~49.4 Gy/26f,1.8~1.9 Gy/f。结果放疗中1例淋巴结局部同步加量患者因放疗局部皮肤反应,中断放疗3周。余患者顺利完成放疗。治疗后随访时间4~54个月,中位随访时间36个月,1例患者治疗结束后4个月死亡,死亡原因不详;2例患者放化疗后出现淋巴结及远处转移,治疗后6个月及10个月后死亡。其余12例患者至今健在,定期随访,未见肿瘤复发转移。随访至今,存活患者中1例出现双下肢的轻度水肿,余11例未发生双下肢水肿,无1例发生肠瘘、放射性直肠炎、放射性膀胱炎等放疗相关远期副反应。结论调强适形放疗治疗外阴癌术后腹股沟淋巴结转移的患者,疗效好,毒副反应可耐受。

Effects of quasi-periodicity on defect modes of photonic crystals

In this letter,the effects of Bragg-mirror quasi-periodicity on the shifts and quality factor in defect modes of photonic crystals are studied by using the transfer matrix method.Furthermore the influence of the quasi-periodicity on electric field enhancement effect inside the defect layer is studied,which is similar to that on the quality factor.

Enhancement effect of phase-conjugate waves of third order nonlinear medium in the Bragg microcavity

The enhancement effect of the phase-conjugate wave produced by a third order nonlinear optical medium in a （AB）^N （D）^2 （BA）^N Bragg microcavity made of one-dimensional photonic crystals is studied. The gain factor of the phase-conjugate wave is derived when the resonance condition is satisfied by the incident optical wave and the Bragg microcavity mode. Because of stronger resonance enhancement to incident pump beam given by the Bragg microcavity, the enhancement effect of the phase-conjugate wave produced by a third order nonlinear optical medium inside Bragg microcavity is more outstanding than that caused by the naked third order nonlinear optical medium. The theoretical analysis and the numerical calculation show the Bragg microcavity with larger number N of periods is identical with a Fabry-Perot cavity when the resonance condition is satisfied by the incident optical wave and the Bragg microcavity mode.

Properties of defect mode and optical enhancement of 1Dphotonic crystals with a defect layer of negative refractiveindex material

The band gap properties of one-dimensional photonic crystals with a defect layer of negative refractive index materials are studied.The defect mode width is bigger than that of conventional one-dimensional photonic crystals with a defect layer of positive refractive index materials.The defect mode of the former is different from that of the latter,shifts towards the direction of high frequency (short wavelength),and has a bigger shifting velocity.Furthermore the effects on the transmission properties of the former photonic crystals caused by change in the position of the defect layer of negative refractive index are investigated.Finally the optical enhancement of the former photonic crystals is also investigated.

下生殖道微生物在宫颈癌发生发展中的研究现状及展望

生殖道微生物是定植于女性子宫及阴道的微生物集合,参与调节生殖道的稳态、代谢、炎症及免疫反应,在维持生殖道健康,抵御病原入侵中扮演重要角色。随着"人类微生物组计划"及其他微生物宏基因组相关项目的开展,人们对生殖道微生物的认识不断深入。研究表明,在HPV获得及持续感染状态、宫颈癌前病变到宫颈癌的发展中,下生殖道--宫颈阴道微生物群的物种多样性增加,乳酸杆菌丰度下调,提示微生物群的失衡与肿瘤发展相关。以乳酸杆菌占主导的微生物群落能促进HPV感染清除,在宫颈癌的防治中显示出潜力。本文基于最新研究进展,对女性下生殖道微生物的组成、功能、与HPV感染及宫颈癌发生发展的关系进行综述,并对该领域有待解决的问题作一分析与展望。