The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulce...The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.展开更多
目的应用Meta分析系统评价超声影像组学诊断乳腺癌患者腋窝淋巴结转移(ALNM)的临床价值。方法计算机检索PubMed、Web of Science、Embase、Cochrane Library、中国知网、万方数据知识服务平台、维普数据库、中国生物医学文献数据库,搜...目的应用Meta分析系统评价超声影像组学诊断乳腺癌患者腋窝淋巴结转移(ALNM)的临床价值。方法计算机检索PubMed、Web of Science、Embase、Cochrane Library、中国知网、万方数据知识服务平台、维普数据库、中国生物医学文献数据库,搜索建库至2023年10月1日关于超声影像组学对乳腺癌患者ALNM的诊断性研究,由2名研究人员筛选文献并提取有效数据,采用Stata 17.0、RevMan 5.3和Meta-Disc 1.4统计软件进行Meta分析。结果本研究最终纳入14篇文献,研究对象包括乳腺癌患者4469例,其中ALNM患者1748例,非ALNM患者2721例。Meta分析结果显示,超声影像组学诊断乳腺癌患者ALNM的合并灵敏度为0.79[95%可信区间(CI):0.73~0.85],合并特异度为0.79(95%CI:0.71~0.85),合并阳性似然比为3.70(95%CI:2.70~5.10),合并阴性似然比为0.26(95%CI:0.19~0.35),合并诊断比值比为14.0(95%CI:8.0~24.0),综合受试者工作特征曲线下面积为0.86(95%CI:0.83~0.89)。合并结果的异质性并非单项研究引起,亚组分析显示,样本量大小和文献发表地可能是造成异质性的原因。Deek’s漏斗图提示不存在潜在的发表偏倚(t=0.37,P=0.72)。Fagan图显示,超声影像组学诊断乳腺癌患者ALNM的验前概率为50%,当阳性似然比为4、阴性似然比为0.26时,其验后概率分别为79%、21%。结论超声影像组学诊断乳腺癌患者ALNM具有一定的临床价值。展开更多
基金supported by the National Key R&D Pro-grams of China(No.2018YFD0901103)the Hainan Provincial Natural Science Foundation of China(No.2019 RC093).
文摘The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.
