The NAC （NAM, ATAF1/2 and CUC2） transcription factor family plays a key role in plant development and responses to abiotic stress. GmNAC15 （Glyma 15g40510.1）, a member of the NAC transcription factor family in soy...The NAC （NAM, ATAF1/2 and CUC2） transcription factor family plays a key role in plant development and responses to abiotic stress. GmNAC15 （Glyma 15g40510.1）, a member of the NAC transcription factor family in soybean, was functionally characterized, especially with regard to its role in salt tolerance. In the present study, qRT-PCR （quantitative reverse transcription PCR） analysis indicated that GmNAC15 was induced by salt, drought, low temperature stress, and ABA treatment in roots and leaves. GmNAC15 overexpression in soybean （Glycine max） hairy roots enhanced salt tolerance. Transgenic hairy roots improved the survival of wild leaves; however, overexpression of GmNAC15 in hairy root couldn＇t influnce the expression level of GmNAC15 in leaf. GmNAC15 regulates the expression levels of genes responsive to salt stress. Altogether, these results provide experimental evidence of the positive effect of GmNAC15 on salt tolerance in soybean and the potential application of genetic manipulation to enhance the salt tolerance of important crops.展开更多
SMALL AUXIN-UP RNAs(SAURs) regulated by abiotic stress play multiple functions in plants. However, the functions of SAURs in abiotic stress are largely unknown. In this study, we cloned a novel SAUR gene, Ta SAUR78, f...SMALL AUXIN-UP RNAs(SAURs) regulated by abiotic stress play multiple functions in plants. However, the functions of SAURs in abiotic stress are largely unknown. In this study, we cloned a novel SAUR gene, Ta SAUR78, from wheat, and we found that Ta SAUR78 interacted with Ta VDAC1(voltage-dependent anion channel). Salt stress decreased expression of Ta SAUR78 and increased expression of Ta VDAC1. Overexpression of Ta SAUR78 enhanced tolerance to salt, drought, and freezing stresses in transgenic Arabidopsis and reduced the accumulation of reactive oxygen species(ROS) under salt stress. Overexpression of Ta VDAC1 enhanced tolerance to salt stress, while decreased tolerance to drought and low temperature stresses in transgenic Arabidopsis. Ta VDAC1 overexpression increased the accumulation of ROS in plants. These results suggested that Ta SAUR78 improved plant tolerance to abiotic stresses by regulating Ta VDAC1. This study generated valuable information on the functions of Ta SAUR78 and Ta VDAC1 in multiple abiotic stresses, which may facilitate the deployment of these genes to enhance crop tolerance to abiotic stresses in the future.展开更多
Hybrid necrosis is the gradual premature death of leaves or plants in certain Fj hybrids of wheat （Triticum aestivum L.）. Comparison of protein expression in necrotic and normal wheat leaves showed that the abundanc...Hybrid necrosis is the gradual premature death of leaves or plants in certain Fj hybrids of wheat （Triticum aestivum L.）. Comparison of protein expression in necrotic and normal wheat leaves showed that the abundance of 33 proteins was changed significantly, and 24 of these proteins were identified. These proteins were involved in plant growth and development, antioxidation, photosynthesis and carbon assimilation, amino acid and protein biosynthesis, cytological signal transduction, DNA and RNA modification, protein transport, folding and assembly according to their functions. The down-regulation of uroporphyrinogen decarboxylase and the up-regulation of lipoxygenases in necrotic leaves may be related to the oxidative stress in the necrotic cells. The heat shock proteins may play the cytoprotective role. The differential expression of photosynthesis and carbon assimilation related proteins indicated chlorophyll biosynthesis and chloroplast development were inhibited and might finally cause the gradual chlorosis and cell death in necrotic leaves. The results of this study give a comprehensive picture of the post-transcriptional response to necrosis in hybrid wheat leaves and serve as a platform for further characterization of gene function and regulation in wheat hybrid necrosis.展开更多
基金supported by the National Key Research and Development Program of China (2016YFD0101005)the Agricultural Science and Technology Program for Innovation Team on Identification and excavation of Elite Crop Germplasm, Chinese Academy of Agricultural Sciences
文摘The NAC （NAM, ATAF1/2 and CUC2） transcription factor family plays a key role in plant development and responses to abiotic stress. GmNAC15 （Glyma 15g40510.1）, a member of the NAC transcription factor family in soybean, was functionally characterized, especially with regard to its role in salt tolerance. In the present study, qRT-PCR （quantitative reverse transcription PCR） analysis indicated that GmNAC15 was induced by salt, drought, low temperature stress, and ABA treatment in roots and leaves. GmNAC15 overexpression in soybean （Glycine max） hairy roots enhanced salt tolerance. Transgenic hairy roots improved the survival of wild leaves; however, overexpression of GmNAC15 in hairy root couldn＇t influnce the expression level of GmNAC15 in leaf. GmNAC15 regulates the expression levels of genes responsive to salt stress. Altogether, these results provide experimental evidence of the positive effect of GmNAC15 on salt tolerance in soybean and the potential application of genetic manipulation to enhance the salt tolerance of important crops.
基金supported by the National Natural Science Foundation of China (31601302)the National Key R&D Program of China (2016YFD0100304)+1 种基金the National Transgenic Key Project from the Ministry of Agriculture of China (2016ZX08010-005)the Agricultural Science and Technology Program for Innovation Team on the Identification and Excavation of Elite Crop Germplasm, Chinese Academy of Agricultural Sciences
文摘SMALL AUXIN-UP RNAs(SAURs) regulated by abiotic stress play multiple functions in plants. However, the functions of SAURs in abiotic stress are largely unknown. In this study, we cloned a novel SAUR gene, Ta SAUR78, from wheat, and we found that Ta SAUR78 interacted with Ta VDAC1(voltage-dependent anion channel). Salt stress decreased expression of Ta SAUR78 and increased expression of Ta VDAC1. Overexpression of Ta SAUR78 enhanced tolerance to salt, drought, and freezing stresses in transgenic Arabidopsis and reduced the accumulation of reactive oxygen species(ROS) under salt stress. Overexpression of Ta VDAC1 enhanced tolerance to salt stress, while decreased tolerance to drought and low temperature stresses in transgenic Arabidopsis. Ta VDAC1 overexpression increased the accumulation of ROS in plants. These results suggested that Ta SAUR78 improved plant tolerance to abiotic stresses by regulating Ta VDAC1. This study generated valuable information on the functions of Ta SAUR78 and Ta VDAC1 in multiple abiotic stresses, which may facilitate the deployment of these genes to enhance crop tolerance to abiotic stresses in the future.
基金supported by the National Natural Science Foundation of China (30440047)the Program for Germplasm Innovation and Utilization from the Ministry of Agriculture of China (NB2010-2130135)
文摘Hybrid necrosis is the gradual premature death of leaves or plants in certain Fj hybrids of wheat （Triticum aestivum L.）. Comparison of protein expression in necrotic and normal wheat leaves showed that the abundance of 33 proteins was changed significantly, and 24 of these proteins were identified. These proteins were involved in plant growth and development, antioxidation, photosynthesis and carbon assimilation, amino acid and protein biosynthesis, cytological signal transduction, DNA and RNA modification, protein transport, folding and assembly according to their functions. The down-regulation of uroporphyrinogen decarboxylase and the up-regulation of lipoxygenases in necrotic leaves may be related to the oxidative stress in the necrotic cells. The heat shock proteins may play the cytoprotective role. The differential expression of photosynthesis and carbon assimilation related proteins indicated chlorophyll biosynthesis and chloroplast development were inhibited and might finally cause the gradual chlorosis and cell death in necrotic leaves. The results of this study give a comprehensive picture of the post-transcriptional response to necrosis in hybrid wheat leaves and serve as a platform for further characterization of gene function and regulation in wheat hybrid necrosis.
