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基于网络药理学和分子对接技术的丹皮酚治疗胃癌的作用机制预测及验证 被引量:2
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作者 张梦军 刘侠 +4 位作者 姜伟洲 黄利敏 侯明超 仇士东 李克明 《食品与药品》 CAS 2023年第1期18-25,共8页
目的利用网络药理学方法分析丹皮酚治疗胃癌的作用机制,并利用分子对接技术进行初步验证。方法利用TCMSP、SwissTargetPrediction和SymMap数据库分别获得化合物丹皮酚的已知作用靶点和预测作用靶点,利用GeneCards、CTD数据库获取胃癌的... 目的利用网络药理学方法分析丹皮酚治疗胃癌的作用机制,并利用分子对接技术进行初步验证。方法利用TCMSP、SwissTargetPrediction和SymMap数据库分别获得化合物丹皮酚的已知作用靶点和预测作用靶点,利用GeneCards、CTD数据库获取胃癌的作用靶点,利用Venny 2.1.0将丹皮酚和胃癌作用靶点进行映射,得到共同靶点,利用SRTING获取靶点之间相互关系,Cytoscape 3.6.2绘制PPI网络,利用DAVID数据库进行富集分析,运用schrodinger软件包将丹皮酚和主要作用靶点进行分子对接验证并进行体外细胞实验研究验证。结果共获得124个丹皮酚治疗胃癌的作用靶点,87条信号通路和蛋白质磷酸化等16个生物学功能、质膜等5个细胞组分,蛋白质均聚活性等9个分子功能。分子对接示丹皮酚与靶点丝裂原活化蛋白激酶1(MAPK1)、丝裂原活化蛋白激酶3(MAPK3)、原癌基因酪氨酸蛋白激酶(SRC)结合能较强。体外细胞实验表明,丹皮酚能显著抑制人胃癌细胞SGC-7901增殖,并使细胞中MAPK1、MAPK3、基质金属蛋白酶-9(MMP9)蛋白表达水平显著下降,且呈现出对浓度与时间的依赖性,浓度越高、培养时间越久,抑制作用越强,蛋白表达水平下降越明显。结论丹皮酚可通过MAPK1、MAPK3、SRC、MMP9等靶点调控cancer中信号通路、cAMP信号通路、cGMP-PKG信号通路通路实现对胃癌的干预作用。 展开更多
关键词 丹皮酚 胃癌 丝裂原活化蛋白激酶1 丝裂原活化蛋白激酶3 酪氨酸蛋白激酶 基质金属蛋白酶-9
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依那西普和传统合成改善病情抗风湿药物序贯治疗对髋关节受累强直性脊柱炎患者的疗效评估 被引量:9
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作者 宋旻恺 肖军 +3 位作者 江伟州 严涛 高嘉文 史占军 《第二军医大学学报》 CAS CSCD 北大核心 2020年第6期668-672,共5页
目的评估肿瘤坏死因子抑制剂(TNFi)和传统合成改善病情抗风湿药物(csDMARD)序贯治疗方案在延缓强直性脊柱炎(AS)患者受累髋关节影像学进展及维持功能方面的疗效。方法选择存在髋关节疼痛、活动受限的活动期AS患者,定期检测炎症反应指标... 目的评估肿瘤坏死因子抑制剂(TNFi)和传统合成改善病情抗风湿药物(csDMARD)序贯治疗方案在延缓强直性脊柱炎(AS)患者受累髋关节影像学进展及维持功能方面的疗效。方法选择存在髋关节疼痛、活动受限的活动期AS患者,定期检测炎症反应指标CRP、红细胞沉降率(ESR)并使用基于CRP的强直性脊柱炎疾病活动度评分(ASDASCRP)评估其疾病活动度,根据疾病活动度序贯使用TNFi依那西普与csDMARD方案。分别于治疗前和治疗后3、6、12个月,使用基于视觉模拟量表的患者整体评价评估受试者主观症状,ASDASCRP、巴氏强直性脊柱炎疾病活动度指数(BASDAI)评估病情,巴氏强直性脊柱炎功能指数(BASFI)评估躯体功能,巴氏强直性脊柱炎髋关节影像学指数(BASRI-hip)及最小关节间隙宽度(mJSW)评估髋关节影像学改变。结果共纳入受试者51例,男38例(74.5%),女13例(25.5%),年龄为10~56岁,发病年龄为9~40岁,均未因髋关节功能受限或关节骨质进一步破坏行髋关节置换术。治疗后3、6、12个月,51例AS患者的国际强直性脊柱炎评价(ASAS)工作组20%缓解标准(ASAS20)缓解率分别为70.59%(36/51)、84.31%(43/51)和96.08%(49/51),ASAS工作组40%缓解标准(ASAS40)缓解率分别为58.82%(30/51)、78.43%(40/51)和86.27%(44/51)。治疗后3、6、12个月,炎症反应指标CRP水平、ESR及患者整体评价评分、ASDASCRP、BASDAI评分、BASFI评分均呈下降趋势(P均<0.05),BASRI评分和mJSW总体趋势无明显变化(P均>0.05)。结论TNFi和csDMARD序贯治疗方案可以有效抑制AS患者的炎症,阻止髋关节破坏进展,改善髋关节功能,保持髋关节间隙稳定。 展开更多
关键词 强直性脊柱炎 依那西普 抗风湿药 髋关节
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Intermediate Mass Dilepton Production in an Expanding Baryon-rich Quark--Gluon Matter
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作者 HE Ze-Jun jiang wei-zhou +1 位作者 ZHOU Wen-Jie ZHANG Jia-Ju 《Chinese Physics Letters》 SCIE CAS CSCD 2001年第2期199-201,共3页
The intermediate mass dileptons from the quark phase,secondary hadronic processes and background sources have been studied based on a relativistic hydrodynamic model.Due to the effect of the phase boundary on the evol... The intermediate mass dileptons from the quark phase,secondary hadronic processes and background sources have been studied based on a relativistic hydrodynamic model.Due to the effect of the phase boundary on the evolution of the system the contribution from the quark phase is much more important than that from secondary processes and is even comparable with that from background sources.This leads to a spectrum without the obvious humps of the hadronic phase contribution and the yield increasing with the incident energy of colliding nuclei.It is shown that the enhancement of dileptons is a signature for the formation of the quark--gluon plasma. 展开更多
关键词 QUARK SIGNATURE RELATIVISTIC
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Total Reaction Cross Section Measurements at Intermediate Energy for the Proton Halo Candidate ^(23)Al and Its Neighbours
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作者 CAI Xiang-Zhou SHEN Wen-Qing +19 位作者 FENG Jun FANG De-Qing ZHU Zhi-Yuan jiang wei-zhou MA Yu-Gang ZHANG Hu-Yong ZHONG Chen YU Li-Ping ZHAN Wen-Long GUO Zhong-Yan XIAO Gu-Qhig WANG Jian-Song ZHU Yong-Tai WANG Jin-Chuan LI Jia-Xing WANG Meng WANG Jian-Feng NING Zhen-jiang WANG Quan-Jin CHEN Zhi-Qiang 《Chinese Physics Letters》 SCIE CAS CSCD 2001年第9期1189-1191,共3页
Total reaction cross sections σR for some proton-rich isotones (N = 10) near ^(23)Al on a carbon target have been measured at intermediate energies in the experiment performed at the Radioactive Ion Beam Line of the ... Total reaction cross sections σR for some proton-rich isotones (N = 10) near ^(23)Al on a carbon target have been measured at intermediate energies in the experiment performed at the Radioactive Ion Beam Line of the Heavy Ion Research Facility in Lanzhou. The Z-dependence of σR shows a remarkable enhancement for ^(23)Al relative to its neighbours. The difference factor d has been deduced from the measured σR by using the Glauber or Boltzmann-Uehling-Uhlenbeck models. From the above analysis, a possible one-proton halo structure is suggested for ^(23)Al. 展开更多
关键词 deduced TOTAL BOLTZMANN
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Dilepton Production in an Expanding Hot Baryon-Rich Quark-Gluon Matter
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作者 jiang wei-zhou HE Ze-jun +1 位作者 ZHU Zhi-yuan ZHANG Jia-ju 《Chinese Physics Letters》 SCIE CAS CSCD 1999年第12期879-881,共3页
Under thermodynamic equilibrium, the initial values of the quark-gluon system from relativistic nucleus-nucleus collisions are obtained. From these initial values we have studied the dilepton production on the basis o... Under thermodynamic equilibrium, the initial values of the quark-gluon system from relativistic nucleus-nucleus collisions are obtained. From these initial values we have studied the dilepton production on the basis of the relativistic hydrodynamic model, and found that with increasing incident energy a characteristic plateau indicating the formation of the quark-gluon plasma appears in the total yield. 展开更多
关键词 RELATIVISTIC QUARK LEPTON
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Dilepton Production in an Expanding Hot Baryon-Rich Quark-Gluon Matter
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作者 He Ze-jun ZHANG Jia-ju +2 位作者 jiang wei-zhou QIU Xi-jun LIU Bo 《Chinese Physics Letters》 SCIE CAS CSCD 1999年第4期259-261,共3页
From the full stopping scenario,a relativistic hydrodynamic model describing the evolution of the baryon-rich quark-gluon matter fire-cylinder has been established.Based on it,the rapidity Distribution of dileptons wi... From the full stopping scenario,a relativistic hydrodynamic model describing the evolution of the baryon-rich quark-gluon matter fire-cylinder has been established.Based on it,the rapidity Distribution of dileptons with intermediate invariant masses has been studied.It is found that the quark phase gives the dominant contribution to the dilepton spectrum due to the influence of the phase boundary on the evolution of the system,especially the dilepton yield is strongly suppressed with the increasing rapidity. 展开更多
关键词 QUARK LEPTON INVARIANT
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Medium Effects on Dynamical Properties of the Fermion and Mesons in the Gauged Nambu- Jona-Lasinio Model
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作者 jiang wei-zhou QIU Xi-jun 《Chinese Physics Letters》 SCIE CAS CSCD 1998年第9期639-641,共3页
The gap equation for the fermion in the gauged Nambu-Jona-Lasinio(NJL)model is derived in nuclear medium with flavors Nf=2.Based on the gap equation,the fermion mass in nuclear medium is obtained and used to determine... The gap equation for the fermion in the gauged Nambu-Jona-Lasinio(NJL)model is derived in nuclear medium with flavors Nf=2.Based on the gap equation,the fermion mass in nuclear medium is obtained and used to determine the properties of the scalar and pseudoscalar mesons.In contrast to the NJL model,the gluonic part has the one-fourth contribution to the fermion mass.The critical density with the chiral symmetry restored is about 6ρ0. 展开更多
关键词 SCALAR EQUATION FERMI
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Relativistic Density-Dependent Hartree Approach for Nuclear Matter in the Chiral-Symmetry-Breaking Model
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作者 jiang wei-zhou ZHU Zhi-yuan QIU Xi-jun 《Chinese Physics Letters》 SCIE CAS CSCD 1996年第6期416-419,共4页
A relativistic density-dependent Hartree approach in the chiral-symmetry-breaking field model has been developed for nuclear matter.The coupling constants of the relativistic Hartree-Lagrangian are made density depend... A relativistic density-dependent Hartree approach in the chiral-symmetry-breaking field model has been developed for nuclear matter.The coupling constants of the relativistic Hartree-Lagrangian are made density dependence and obtained from the relativistic Brueckner-Bethe-Goldstone results of nuclear matter.The calculated saturation nuclear density,binding-energy and compressibility for nuclear matter are close to the empirical ones. 展开更多
关键词 HARTREE RELATIVISTIC CHIRAL
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