Hrr25是酿酒酵母中Ⅰ型酪蛋白激酶家族成员,具有丝氨酸/苏氨酸蛋白激酶活性。其可以通过磷酸化多种蛋白质来发挥作用。Hrr25的底物蛋白质包括自噬相关蛋白、COPⅡ(coat protein complexes Ⅱ)囊泡衣被蛋白Sec24和Sec23、真核翻译起始因...Hrr25是酿酒酵母中Ⅰ型酪蛋白激酶家族成员,具有丝氨酸/苏氨酸蛋白激酶活性。其可以通过磷酸化多种蛋白质来发挥作用。Hrr25的底物蛋白质包括自噬相关蛋白、COPⅡ(coat protein complexes Ⅱ)囊泡衣被蛋白Sec24和Sec23、真核翻译起始因子6、γ-微管蛋白Tub4、延长器复合蛋白1等。另外Hrr25还可以与减数分裂重组蛋白Rec8、核孔蛋白Nup53、转录调节因子Crz1、转录激活因子Haa1等相互作用。Hrr25的多种相互作用蛋白质使其在自噬、囊泡运输、微管组装、减数分裂、有丝分裂、DNA修复、核糖体生物发生和弱有机酸胁迫途径等多种生物过程发挥作用。为了更好地了解Hrr25在各个生物过程中的作用机制以及各个生物过程之间的联系,本文总结了Hrr25的生物学功能及其作用机制,并概述其研究的潜在意义,为Hrr25的进一步研究提供理论依据。展开更多
【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechan...【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechanism of A.subulatus Michx.【Method】After cultured for 24 d under the action of Hoagland nutrient solution and gradient Cd concentrations(0,30,60 and 90 mg/L),A.subulatus Michx.were harvested,and its leaf,stem and root were treated by differential centrifugation,chemical reagent extraction,and digested with graphite digester,respectively,then the Cd content in the root,stem and leaf were determined by atomic absorption spectroscopy.【Result】The experimental results indicated that the bioaccumulation capacity of Cd in A.subulatus Michx.was root>stem>leaf,and the maximum Cd concentration in the root,stem and leaf of A.subulatus Michx.were 130.74,78.69 and 56.62 mg/kg(fresh matter),respectively.Most of Cd stored in the cell wall and the soluble fractions of the root and leaf of A.subulatus Michx.,with only a smaller portion Cd in organelle fraction.Analysis result of subcellular Cd content showed that 52.27%-58.61%of Cd for root was mainly stored in the soluble fraction,but 42.10%-63.28%of Cd for leaf was mainly stored in the cell wall fraction.The concentration of pectates and protein integrated-Cd was higher in the root and leaf compared to other chemical forms Cd.Pectates and protein integrated-Cd was the main chemical forms Cd in the root and leaf of A.subulatus Michx.,and their percentages were 68.91%-74.80%and 57.38%-83.80%,respectively.Cd treatment could significantly increase the proportion of water-soluble organic acid Cd from 13.64%to 22.72%in root and undissolved phosphate Cd from 10.02%to 32.78%in leaf with increasing Cd concentration in the culture medium.【Conclusion】The root,stem and leaf of A.subulatus Michx.has strong bioaccumulation capacity to Cd,Cd is primarily stored in the soluble fractions of the root and cell wall fractions of the leaf,and less toxic pectates and protein integrated-Cd is the main chemical forms Cd in the root and leaf of A.subulatus Michx.,this might be the main mechanism of Cd tolerance in A.subulatus Michx.展开更多
The coastal plain region usually shows the agricultural dominated industry mode, so more and more attention is paid to non-point pollutants discharge. In this study, the method for assessing the influence of non-point...The coastal plain region usually shows the agricultural dominated industry mode, so more and more attention is paid to non-point pollutants discharge. In this study, the method for assessing the influence of non-point pollutants discharge on river water quality is probed which is concluded as follows： （1） Considering the costal plain island is characterized as nearly even elevation, the conventional gravitational runoff confluence method based on eight-point gravitational flowing directions （DS） is unable to compute the runoff confluence received by the surrounding rivers, so a new method of triangular confluence based on three-points of grid center and the river segments is presented. Further the equations of non-point pollutants loads estimation and non-point pollutants confluence on rivers are presented. （2） The integration mode of non-point pollutants model and river water quality model is presented so as to further assess the non-point pollutants contribution to river water quality. （3） With the Chongming Island, the third largest island in China, as an example, the above-mentioned equations are specified. For this island, the non-point pollutants loads are estimated and linked to the developed water quality model of the river network in the island, and further the non-point pollution evolution in river network is simulated. In this scenario, the non-point pollution contribution to river water quality over the whole island is clearly displayed, and the area where the river water quality is seriously influenced by non-point pollutants discharge is distinctly depicted. This scenario also shows the water quality contribution ratio of non-point pollution to point pollution can be in the range of 55.5% to 44.5% which proves the importance of non-point pollution control in costal plain islands.展开更多
文摘Hrr25是酿酒酵母中Ⅰ型酪蛋白激酶家族成员,具有丝氨酸/苏氨酸蛋白激酶活性。其可以通过磷酸化多种蛋白质来发挥作用。Hrr25的底物蛋白质包括自噬相关蛋白、COPⅡ(coat protein complexes Ⅱ)囊泡衣被蛋白Sec24和Sec23、真核翻译起始因子6、γ-微管蛋白Tub4、延长器复合蛋白1等。另外Hrr25还可以与减数分裂重组蛋白Rec8、核孔蛋白Nup53、转录调节因子Crz1、转录激活因子Haa1等相互作用。Hrr25的多种相互作用蛋白质使其在自噬、囊泡运输、微管组装、减数分裂、有丝分裂、DNA修复、核糖体生物发生和弱有机酸胁迫途径等多种生物过程发挥作用。为了更好地了解Hrr25在各个生物过程中的作用机制以及各个生物过程之间的联系,本文总结了Hrr25的生物学功能及其作用机制,并概述其研究的潜在意义,为Hrr25的进一步研究提供理论依据。
基金Guangxi Natural Science Foundation(2024GXNSFAA010469,2021GXNSFBA196028)Science and Technology Development Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021YT137,Guinongke 2022JM86)。
文摘【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechanism of A.subulatus Michx.【Method】After cultured for 24 d under the action of Hoagland nutrient solution and gradient Cd concentrations(0,30,60 and 90 mg/L),A.subulatus Michx.were harvested,and its leaf,stem and root were treated by differential centrifugation,chemical reagent extraction,and digested with graphite digester,respectively,then the Cd content in the root,stem and leaf were determined by atomic absorption spectroscopy.【Result】The experimental results indicated that the bioaccumulation capacity of Cd in A.subulatus Michx.was root>stem>leaf,and the maximum Cd concentration in the root,stem and leaf of A.subulatus Michx.were 130.74,78.69 and 56.62 mg/kg(fresh matter),respectively.Most of Cd stored in the cell wall and the soluble fractions of the root and leaf of A.subulatus Michx.,with only a smaller portion Cd in organelle fraction.Analysis result of subcellular Cd content showed that 52.27%-58.61%of Cd for root was mainly stored in the soluble fraction,but 42.10%-63.28%of Cd for leaf was mainly stored in the cell wall fraction.The concentration of pectates and protein integrated-Cd was higher in the root and leaf compared to other chemical forms Cd.Pectates and protein integrated-Cd was the main chemical forms Cd in the root and leaf of A.subulatus Michx.,and their percentages were 68.91%-74.80%and 57.38%-83.80%,respectively.Cd treatment could significantly increase the proportion of water-soluble organic acid Cd from 13.64%to 22.72%in root and undissolved phosphate Cd from 10.02%to 32.78%in leaf with increasing Cd concentration in the culture medium.【Conclusion】The root,stem and leaf of A.subulatus Michx.has strong bioaccumulation capacity to Cd,Cd is primarily stored in the soluble fractions of the root and cell wall fractions of the leaf,and less toxic pectates and protein integrated-Cd is the main chemical forms Cd in the root and leaf of A.subulatus Michx.,this might be the main mechanism of Cd tolerance in A.subulatus Michx.
基金supported by the Scientific Research Project of Chongming Island of Shanghai Science and Technology Commision(Grant No. 06dz12037)
文摘The coastal plain region usually shows the agricultural dominated industry mode, so more and more attention is paid to non-point pollutants discharge. In this study, the method for assessing the influence of non-point pollutants discharge on river water quality is probed which is concluded as follows： （1） Considering the costal plain island is characterized as nearly even elevation, the conventional gravitational runoff confluence method based on eight-point gravitational flowing directions （DS） is unable to compute the runoff confluence received by the surrounding rivers, so a new method of triangular confluence based on three-points of grid center and the river segments is presented. Further the equations of non-point pollutants loads estimation and non-point pollutants confluence on rivers are presented. （2） The integration mode of non-point pollutants model and river water quality model is presented so as to further assess the non-point pollutants contribution to river water quality. （3） With the Chongming Island, the third largest island in China, as an example, the above-mentioned equations are specified. For this island, the non-point pollutants loads are estimated and linked to the developed water quality model of the river network in the island, and further the non-point pollution evolution in river network is simulated. In this scenario, the non-point pollution contribution to river water quality over the whole island is clearly displayed, and the area where the river water quality is seriously influenced by non-point pollutants discharge is distinctly depicted. This scenario also shows the water quality contribution ratio of non-point pollution to point pollution can be in the range of 55.5% to 44.5% which proves the importance of non-point pollution control in costal plain islands.
