The brown planthopper(Nilaparvata lugens) is the main migratory pest in many rice growing areas in Asia. E78 is a member of the nuclear hormone receptor superfamily which plays an important role in egg development and...The brown planthopper(Nilaparvata lugens) is the main migratory pest in many rice growing areas in Asia. E78 is a member of the nuclear hormone receptor superfamily which plays an important role in egg development and maternal regulation of early embryogenesis. In this study, brown planthopper E78(NlE78) was cloned, and the predicted amino acid sequence showed that it contains two conserved domains: NR-LBD and DBD. qRT-PCR showed that the expression of NlE78 is high in the fifth instar nymphs and the ovaries of females. After downregulation of NlE78, the rate of moulting failure(33.2%) increased significantly, and ovarian development was delayed. However, when NlE78was downregulated together with NlE93, the emergence rate increased significantly(78.79%), and ovarian development was similar to that when NlE78 was downregulated but not delayed. A co-immunoprecipitation experiment showed that NlE78 interacts with NlE93, a crucial downstream transcription factor of the ecdysone signalling pathway. Cellular localization by immunofluorescence revealed that NlE78 and NlE93 are expressed in the nucleus. This study indicates that NlE78 regulates ovarian development and moulting, possibly through its interaction with NlE93. This study is of great significance for the development of new pesticides and control methods based on newly discovered targets.展开更多
在低能基准辐射场,利用RC6M平板型电离室构建了W靶Mo过滤乳腺诊断的五个辐射质,并完成了半值层、同质系数的测量。测量和模拟了所建辐射质能谱。结果显示所建辐射质半值层范围在0.34～0.405 mm,有效能量维持在16 ke V左右。实测能谱与...在低能基准辐射场,利用RC6M平板型电离室构建了W靶Mo过滤乳腺诊断的五个辐射质,并完成了半值层、同质系数的测量。测量和模拟了所建辐射质能谱。结果显示所建辐射质半值层范围在0.34～0.405 mm,有效能量维持在16 ke V左右。实测能谱与模拟能谱相似度高。完成了W靶Mo过滤乳腺诊断辐射质空气比释动能溯源的初期工作。展开更多
基金supported by the National Natural Science Foundation of China (32172390, 1672023 and 31741107)the Natural Science Foundation of Zhejiang Province, China (LZ20C140002)。
文摘The brown planthopper(Nilaparvata lugens) is the main migratory pest in many rice growing areas in Asia. E78 is a member of the nuclear hormone receptor superfamily which plays an important role in egg development and maternal regulation of early embryogenesis. In this study, brown planthopper E78(NlE78) was cloned, and the predicted amino acid sequence showed that it contains two conserved domains: NR-LBD and DBD. qRT-PCR showed that the expression of NlE78 is high in the fifth instar nymphs and the ovaries of females. After downregulation of NlE78, the rate of moulting failure(33.2%) increased significantly, and ovarian development was delayed. However, when NlE78was downregulated together with NlE93, the emergence rate increased significantly(78.79%), and ovarian development was similar to that when NlE78 was downregulated but not delayed. A co-immunoprecipitation experiment showed that NlE78 interacts with NlE93, a crucial downstream transcription factor of the ecdysone signalling pathway. Cellular localization by immunofluorescence revealed that NlE78 and NlE93 are expressed in the nucleus. This study indicates that NlE78 regulates ovarian development and moulting, possibly through its interaction with NlE93. This study is of great significance for the development of new pesticides and control methods based on newly discovered targets.
文摘在低能基准辐射场,利用RC6M平板型电离室构建了W靶Mo过滤乳腺诊断的五个辐射质,并完成了半值层、同质系数的测量。测量和模拟了所建辐射质能谱。结果显示所建辐射质半值层范围在0.34～0.405 mm,有效能量维持在16 ke V左右。实测能谱与模拟能谱相似度高。完成了W靶Mo过滤乳腺诊断辐射质空气比释动能溯源的初期工作。
