We compared the numbers of nucleotide substitutions occurring in the non-coding regions and coding regions of Ebola virus genomes and found that non-coding regions contain indispensable phylogenetic and evolutionary i...We compared the numbers of nucleotide substitutions occurring in the non-coding regions and coding regions of Ebola virus genomes and found that non-coding regions contain indispensable phylogenetic and evolutionary information. The omission of genetic data from non-coding regions can lead to unreliable phylogenies and inaccurate estimates of evolutionary parameters.展开更多
The bioactive borosilicate scaffolds (R2O-RO-B2O3-SiO2-P2O5) with four different contents of borate were fabricated by replication technique. The bioactivity, degradability and the cytotoxicity of the scaffolds were s...The bioactive borosilicate scaffolds (R2O-RO-B2O3-SiO2-P2O5) with four different contents of borate were fabricated by replication technique. The bioactivity, degradability and the cytotoxicity of the scaffolds were studied in this paper. The porosity of the scaffolds was found to be 73%―80%, and the pore size was in the range of 200―300 μm. The porous scaffolds immersed in 0.02 mol·L-1 K2HPO4 solution were transformed into hydroxyapatite. And it is notable that the D-Alk-2B, D-Alk-3B-scaffolds were covered by hydroxyapatite layers after 7 h-immersion, which proved their high bioactivity. In the cell adhesion test, cells could be seen growing well on the scaffolds, showing stretched morphology and obvious pseudopodia, and only the high cumulative concentration of B ions released from the D-Alk-3B-scaffold samples had an inhibition effect on cell proliferation. But the inhibition effect could be alleviated by diluting the extract solution to a certain concentration (dilution ratio: 1:8). Therefore, after suitable pretreatment, the porous borosilicate bioactive glass scaffold can be a desirable candidate for bone tissue engineering.展开更多
基金supported by the National Natural Science Foundation of China(81470096)the Doctoral Starting up Foundation of Taishan Medical Collegesupported by a grant from the International Development Research Centre
文摘We compared the numbers of nucleotide substitutions occurring in the non-coding regions and coding regions of Ebola virus genomes and found that non-coding regions contain indispensable phylogenetic and evolutionary information. The omission of genetic data from non-coding regions can lead to unreliable phylogenies and inaccurate estimates of evolutionary parameters.
基金Supported by the National Basic Research Program of China (Grant No. 05DJ14006)Biology and Pharmacy Strategic Project Shanghai Science & Technology Commission (Grant No. 08441900500)
文摘The bioactive borosilicate scaffolds (R2O-RO-B2O3-SiO2-P2O5) with four different contents of borate were fabricated by replication technique. The bioactivity, degradability and the cytotoxicity of the scaffolds were studied in this paper. The porosity of the scaffolds was found to be 73%―80%, and the pore size was in the range of 200―300 μm. The porous scaffolds immersed in 0.02 mol·L-1 K2HPO4 solution were transformed into hydroxyapatite. And it is notable that the D-Alk-2B, D-Alk-3B-scaffolds were covered by hydroxyapatite layers after 7 h-immersion, which proved their high bioactivity. In the cell adhesion test, cells could be seen growing well on the scaffolds, showing stretched morphology and obvious pseudopodia, and only the high cumulative concentration of B ions released from the D-Alk-3B-scaffold samples had an inhibition effect on cell proliferation. But the inhibition effect could be alleviated by diluting the extract solution to a certain concentration (dilution ratio: 1:8). Therefore, after suitable pretreatment, the porous borosilicate bioactive glass scaffold can be a desirable candidate for bone tissue engineering.
