Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and a...Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and atherosclerosis; and inhibition of MMPs may have therapeutic benefits. The discovery of MMP inhibitors from herbal has become a prospective event. We showed that the extract of Polygoni multiflori caulis from ethyl acetate or water（ethyl acetate extract and water extract） can inhibit the activities of MMPs 9, 14, and 16 in a dose-dependent manner and n-butyl alcohol extract of it can also inhibit these MMPs. Furthermore, we found that n-butyl alcohol extract and water extract of it influence the cell viability. These discoveries may contribute to the development of MMP in- hibitors for the therapy of a variety of pathological conditions.展开更多
Matrix metalloproteinases （MMPs） are known to be involved in a number of pathological processes including cancer, atherosclerosis, arthritis and neurodegenerative disease among others. The drive to develop MMP inhib...Matrix metalloproteinases （MMPs） are known to be involved in a number of pathological processes including cancer, atherosclerosis, arthritis and neurodegenerative disease among others. The drive to develop MMP inhibitors as therapeutics has been put forward for years by pharmaceutical companies as well as academicians. In an attempt to screen for MMP inhibitors from Traditional Chinese Medicines （TCMs） , a number of Chinese formulations used to treat inflammatory diseases such as nephritis and hepatitis have been studied. Strong inhibitory effects of three Chinese formulations toward the activity of MMP-16 have been discovered. These results suggest that these anti-inflammatory medicines contain some unknown MMP inhibitory compound（s） and provide reasonable molecular mechanisms for their theraoeutic effects.展开更多
Matrix metalloproteinases（MMPs） are a family of proteases that are required for many biological processes and are also elevated in many pathological conditions. MMP inhibitors （MMPIs） may therefore be useful as th...Matrix metalloproteinases（MMPs） are a family of proteases that are required for many biological processes and are also elevated in many pathological conditions. MMP inhibitors （MMPIs） may therefore be useful as therapeutic agents in treating a number of diseases including cancer, cardiovascular diseases and arthritis. Attempts have been made to develop MMPIs. Recombinant MMPs have been used to screening MMPs in vitro assays. In this work, we report the expression of MMP-16 in E. coli and the characterization of the recombinant MMP-16 with a commonly used MMP substrate DQ-gelatin.展开更多
基金Supported by the National Natural Science Foundation of China(No.30571656).
文摘Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and atherosclerosis; and inhibition of MMPs may have therapeutic benefits. The discovery of MMP inhibitors from herbal has become a prospective event. We showed that the extract of Polygoni multiflori caulis from ethyl acetate or water（ethyl acetate extract and water extract） can inhibit the activities of MMPs 9, 14, and 16 in a dose-dependent manner and n-butyl alcohol extract of it can also inhibit these MMPs. Furthermore, we found that n-butyl alcohol extract and water extract of it influence the cell viability. These discoveries may contribute to the development of MMP in- hibitors for the therapy of a variety of pathological conditions.
文摘Matrix metalloproteinases （MMPs） are known to be involved in a number of pathological processes including cancer, atherosclerosis, arthritis and neurodegenerative disease among others. The drive to develop MMP inhibitors as therapeutics has been put forward for years by pharmaceutical companies as well as academicians. In an attempt to screen for MMP inhibitors from Traditional Chinese Medicines （TCMs） , a number of Chinese formulations used to treat inflammatory diseases such as nephritis and hepatitis have been studied. Strong inhibitory effects of three Chinese formulations toward the activity of MMP-16 have been discovered. These results suggest that these anti-inflammatory medicines contain some unknown MMP inhibitory compound（s） and provide reasonable molecular mechanisms for their theraoeutic effects.
文摘Matrix metalloproteinases（MMPs） are a family of proteases that are required for many biological processes and are also elevated in many pathological conditions. MMP inhibitors （MMPIs） may therefore be useful as therapeutic agents in treating a number of diseases including cancer, cardiovascular diseases and arthritis. Attempts have been made to develop MMPIs. Recombinant MMPs have been used to screening MMPs in vitro assays. In this work, we report the expression of MMP-16 in E. coli and the characterization of the recombinant MMP-16 with a commonly used MMP substrate DQ-gelatin.
