To search for certain signature proteins and the expression profiles in lacrimal passage stenosis,rabbit models of lacrimal passage stenosis were treated by 125I seed brachytherapy.All the signature proteins were sepa...To search for certain signature proteins and the expression profiles in lacrimal passage stenosis,rabbit models of lacrimal passage stenosis were treated by 125I seed brachytherapy.All the signature proteins were separated by two-dimensional electrophoresis,and identified by mass spectrometry.The results show that the up-regulated proteins are peptidyl-prolyl cis-trans isomerase A(PPIase A),and epidermal fatty acid-binding protein(E-FABP),while the down-regulated proteins are myosin light chain 1(isomer of skeletal muscle),myosin light polypeptide 6(isomer 1 of smooth muscle and non-muscle),myosin light chain 1(isomer of slow-twitch muscle A),isomer 2 of ERC protein 2,and α-crystallin family protein.The proteins may play a role in healing the wound and regulating synaptic active zone of neurons due to correlation to cell apoptosis,proliferation and migration of smooth muscle cell.These provide molecular mechanism for preventing stenosis and restenosis of lacrimal passage.展开更多
To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(Ne...To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ^(125)I radioactive probing with the ^(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ^(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ^(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.展开更多
基金Supported by Nature Science Foundation of Jilin Province(No.20075323)Project of Science and Technology of Changchun City(2007GH25)
文摘To search for certain signature proteins and the expression profiles in lacrimal passage stenosis,rabbit models of lacrimal passage stenosis were treated by 125I seed brachytherapy.All the signature proteins were separated by two-dimensional electrophoresis,and identified by mass spectrometry.The results show that the up-regulated proteins are peptidyl-prolyl cis-trans isomerase A(PPIase A),and epidermal fatty acid-binding protein(E-FABP),while the down-regulated proteins are myosin light chain 1(isomer of skeletal muscle),myosin light polypeptide 6(isomer 1 of smooth muscle and non-muscle),myosin light chain 1(isomer of slow-twitch muscle A),isomer 2 of ERC protein 2,and α-crystallin family protein.The proteins may play a role in healing the wound and regulating synaptic active zone of neurons due to correlation to cell apoptosis,proliferation and migration of smooth muscle cell.These provide molecular mechanism for preventing stenosis and restenosis of lacrimal passage.
基金Supported by Natural Science Foundation of Jilin Province(No.200705327)Project of Science and Technology of Changchun City(2007GH25)
文摘To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ^(125)I radioactive probing with the ^(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ^(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ^(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.
