In this study, protoplasts were successfully isolated from Kappaphycus alvarezii using snail enzymes, abalone enzymes and cellulase. The optimum enzymic ratio was fixed to be 20% of abalone enzyme, 12% of cellulase an...In this study, protoplasts were successfully isolated from Kappaphycus alvarezii using snail enzymes, abalone enzymes and cellulase. The optimum enzymic ratio was fixed to be 20% of abalone enzyme, 12% of cellulase and the osmotic stabilizer was 2.0 mol/L glucose. The optimum enzymic hydrolysis conditions were found to be dark enzymolysis at 30°C continuing for 4.0 h. The resultant density and yield of protoplasts achieved 32.60×10^4 mL-1, 65.20×10^4 g-1 tissue for Kappaphycus alvarezii. Finally, under the temperature of 20°C, light intensity of 1 500–2 000 lx and photoperiod of 12 h/d, two developmental pathways were investigated：（1） callus-like cell mass and regenerated plantlet occurred on protoplast;（2） young shoots and calluslike cell mass occurred in tissue blocks after enzymolysis.展开更多
The aim of this paper was to investigate the rela-tionship between the expression of adrenomedullin(ADM)and microvessel density(MVD)and prognosis in smooth muscle tumor of uterus.The expression ofADM was detected usin...The aim of this paper was to investigate the rela-tionship between the expression of adrenomedullin(ADM)and microvessel density(MVD)and prognosis in smooth muscle tumor of uterus.The expression ofADM was detected using immunohistochemical staining in specimens from 15 normal controls,28 cases of uterine leiomyoma(LE)and 19 cases of uterine leiomyosarcoma(LES).The MVD was assayed by immunostainting with CD34.There was a positive correlation between the ADM expression and MVD in LE and LES respectively(r_(s)=0.823,P<0.01;r_(s)=0.793,P<0.01).The expression of ADM in LE was statistically lower than that in LES(P<0.05).There was a positive cor-relation between the ADM expression and mitotic figures in LES(P<0.05):the more mitotic figures,the higher levels of the ADM expression and poor prognosis.The ADM is an important angiogenic factor in smooth muscle tumor of uterus.The ADM can be used as an accessory marker in esti-mating the malignant potency of LE and judging the prognosis of LES,and as a novel molecular target of anti-angiogenic and anticarcinogenic strategies.展开更多
基金The National Science Foundation Project under contract No.2007FY210500the National Department Public Benefit Research Foundation of China under contract No.200805075+2 种基金the Province Science and Technology in the Guangdong Project under contract Nos 2010B060200010 and 2010B020201015the Science Expenditure in the Hainan Project under contract No.11-20410-0015the National Natural Science Foundation of China under contract Nos 41206106 and 41222038
文摘In this study, protoplasts were successfully isolated from Kappaphycus alvarezii using snail enzymes, abalone enzymes and cellulase. The optimum enzymic ratio was fixed to be 20% of abalone enzyme, 12% of cellulase and the osmotic stabilizer was 2.0 mol/L glucose. The optimum enzymic hydrolysis conditions were found to be dark enzymolysis at 30°C continuing for 4.0 h. The resultant density and yield of protoplasts achieved 32.60×10^4 mL-1, 65.20×10^4 g-1 tissue for Kappaphycus alvarezii. Finally, under the temperature of 20°C, light intensity of 1 500–2 000 lx and photoperiod of 12 h/d, two developmental pathways were investigated：（1） callus-like cell mass and regenerated plantlet occurred on protoplast;（2） young shoots and calluslike cell mass occurred in tissue blocks after enzymolysis.
文摘The aim of this paper was to investigate the rela-tionship between the expression of adrenomedullin(ADM)and microvessel density(MVD)and prognosis in smooth muscle tumor of uterus.The expression ofADM was detected using immunohistochemical staining in specimens from 15 normal controls,28 cases of uterine leiomyoma(LE)and 19 cases of uterine leiomyosarcoma(LES).The MVD was assayed by immunostainting with CD34.There was a positive correlation between the ADM expression and MVD in LE and LES respectively(r_(s)=0.823,P<0.01;r_(s)=0.793,P<0.01).The expression of ADM in LE was statistically lower than that in LES(P<0.05).There was a positive cor-relation between the ADM expression and mitotic figures in LES(P<0.05):the more mitotic figures,the higher levels of the ADM expression and poor prognosis.The ADM is an important angiogenic factor in smooth muscle tumor of uterus.The ADM can be used as an accessory marker in esti-mating the malignant potency of LE and judging the prognosis of LES,and as a novel molecular target of anti-angiogenic and anticarcinogenic strategies.
