LA-D-B1,a novel Abemaciclib derivative,exerts anti-breast cancer effects through CDK4/6

Background:Regulatory proteins involved in human cellular division and proliferation,cyclin-dependent kinases 4 and 6(CDK4/6)are overexpressed in numerous cancers,including triple-negative breast cancer(TNBC).TNBC is a common pathological subtype of breast cancer that is prone to recurrence and metastasis,and has a single treatment method.As one of the CDK4/6 inhibitors,abemaciclib can effectively inhibit the growth of breast tumors.In this study,we synthesized LA-D-B1,a derivative of Abemaciclib,and investigated its anti-tumor effects in breast cancer.Methods:Cellular viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Cell cloning and migration abilities were determined by colony formation assay and wound healing assay.Cell invasion abilities and adhesion were determined by cell invasion assay and cell adhesion assay.The impact of compound LA-D-B1 on cell proliferation and the cell cycle was analyzed through Western blotting,which quantified the levels of proteins associated with the cyclin-dependent kinase(CDK)4/6-cyclin D-Rb-E2F pathway.The in vivo anti-tumor activity of compound LA-D-B1 was investigated using a chick chorioallantoic membrane(CAM)model.Results:The study demonstrated that LA-D-B1 effectively suppressed breast cancer cell proliferation,induced apoptosis,and caused cell cycle arrest.Furthermore,LA-D-B1 reduced the expression of key proteins in the CDK4/6-cyclin D-Rb-E2F pathway,including CDK4,CDK6 and E2F1.The results also indicated significant antitumor activity of LA-D-B1 in a transplanted tumor model.Conclusion:In this study,LA-D-B1 demonstrated a potent anti-tumor effect by effectively suppressing cell proliferation and inhibiting cell cycle progression in breast cancer.These findings highlight the potential of LA-D-B1 as a valuable compound for enhancing therapeutic outcomes and controlling the progression of breast cancer.

脑电生物反馈治疗孤独症谱系障碍共病注意缺陷多动障碍患儿的临床效果

目的探究脑电生物反馈治疗孤独症谱系障碍(ASD)共病注意缺陷多动障碍患儿的临床效果。方法选取西安中医脑病医院收治的79例ASD共病注意缺陷多动障碍患儿,采用随机数字表法将其分为对照组(n=39)和观察组(n=40)。对照组给予常规康复治疗,观察组在对照组基础上给予脑电生物反馈治疗。比较两组的治疗效果。结果观察组的治疗总有效率显著高于对照组(P<0.05)。治疗后,两组的品行问题、学习问题、心身障碍、冲动-多动、焦虑评分及Conners多动指数(CIH)总分均降低(P<0.05);治疗后,观察组的学习问题、心身障碍、冲动-多动评分及CIH总分均低于对照组(P<0.05)。治疗后,观察组的韦氏儿童智力量表(C-WISC)评分高于对照组,脑电生物反馈θ/β值低于对照组(P<0.05)。结论脑电生物反馈用于ASD共病注意缺陷多动障碍患儿治疗中可降低多动指数,改善注意力及智力水平。

基于Citespace的中医药治疗小儿厌食可视化分析

目的梳理30余年中医药治疗小儿厌食的研究状况及趋势。方法检索中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(万方数据)、中国生物医学文献服务系统(SinoMed)自建库至2022年10月的中医药治疗小儿厌食的相关文献,应用Citespace 6.1.R3对纳入文献的作者、研究机构及关键词进行可视化分析。结果经筛选,共纳入725篇文献,涉及772位作者。学者自1995年开始重视研究小儿厌食领域,形成了分别以汪受传、杜永平、万力生、向希雄、史正刚、夏以琳、史健、孙远岭为代表的研究团队等;发文机构以南京中医药大学(28)、北京中医药大学(25)、成都中医药大学(23)、广州中医药大学(22)为主;研究机构中南京中医药大学、江苏省中医院和深圳市儿童医院有合作;关键词共形成18个主要聚类标签,研究热点主要集中在小儿厌食、临床疗效、用药规律、文献研究、中医药、数据挖掘。结论中医药治疗小儿厌食研究主要集中于临床治疗及临床疗效方面。团队、机构及作者间合作较少,应加强合作,今后研究小儿厌食的趋势主要在临床疗效、文献研究、用药规律、临床观察、数据挖掘等方面。

中医药治疗小儿厌食症的研究进展

该文总结分析近年来中医药治疗小儿厌食症的相关研究,发现小儿厌食症的病因病机涉及先天与后天因素,病变责之于脾胃,病机关键为脾胃不和,纳运失常;临床证型包括邪热不清证、湿滞中焦证、肝气犯胃证、伤食积滞证等;治疗方法主要包括推拿、针刺、穴位贴敷等外治法,经典方药、自拟方、中成药口服及中医食疗等内治法,以及内外合治法。

HPLC法同时测定香云肝泰片中鸟苷和腺苷的含量

目的:分析香云肝泰片中鸟苷及腺苷的含量。方法:采用高效液相色谱法(HPLC),以水和乙腈为流动相,检测波长为260 nm,体积流量为0.5 ml/min,柱温30℃。结果:通过平行测定,6个批号香云肝泰片均检出鸟苷及腺苷,其鸟苷平均含量为0.1069 mg/g,腺苷平均含量为0.1112 mg/g。结论:本检验方法简便、准确、重现性好,可作为香云肝泰片中主要核苷类成分含量的质量检测方法。

Cloning and Functional Characterisation of Carotenoid Cleavage Dioxygenase 4 from Wolfberry

Carotenoid cleavage dioxygenases (CCDs) are a class of enzymes in plants involved in the biosynthesis of apocarotenoids, such as phytohormones, flavour compounds, and other compounds with yet unknown functions. To date, several CCDs have been functionally characterised in plants, but little is known about the CCD4 members. A carotenoid cleavage dioxygenase 4 gene (LcCCD4) was isolated from the leaves of wolfberry (Lycium chinense) to gain insight into its biological function. Multiple sequence alignment and phylogenetic analyses showed that the deduced amino acid sequence of LcCCD4 shares high homology with that of CCD4 proteins from other plants. Expression analysis using semi-quantitative polymerase chain reaction revealed that LcCCD4 was strongly expressed in leaves and flowers and that the expression level was in accordance with β-carotene concentration. LcCCD4 transcripts in fruits tended to decrease as carotenoids accumulated. Recombinant expression of LcCCD4 cleaved β-carotene to produce β-ionone in in vivo assays. These results show that LcCCD4 is a CCD gene that may be involved in producing aromatic apocarotenoids in leaves and flowers, whereas it may be involved in controlling carotenoid accumulation in fruits. © 2016, Tianjin University and Springer-Verlag Berlin Heidelberg.

一氧化氮吸入联合高频通气对呼吸窘迫综合征患儿血气状态及并发症的影响

目的探讨吸入一氧化氮(NO)联合高频振荡通气(HFOV)方案用于重度呼吸窘迫综合征(RDS)患儿的疗效及安全性。方法选取2016年2月—2019年2月于辽宁省健康产业集团阜新矿总医院收治的62例重度RDS患儿。按治疗方式分为两组,30例接受常频通气+NO吸入治疗患儿作为对照组,32例接受HFOV+NO吸入治疗患儿作为研究组,分析两组患儿血气状态改善、并发症等情况。结果研究组呼吸机治疗时间较对照组长(P<0.05)。研究组治疗后的pH、SaO2、PaO2、OI较对照组高(P<0.05),PaCO2、肺泡-动脉血氧分压差较对照组低(P<0.05)。研究组治疗后OI较对照组低(P<0.05)。两组并发症发生率比较,差异无统计学意义(P>0.05)。结论 HFOV+NO可有效改善RDS患儿血气状态,并发症较少,获得良好的疗效及安全性。

Effect of exogenous application of salicylic acid on the drought stress responses of Gardenia jasminoides

The alleviative effects of exogenous salicylic acid （SA） on plants against drought stress were assessed in Gardenia jasminoides seedlings treated with different concentrations of SA. Drought stress was simulated to a moderate level by 15% polyethylene glycol （PEG） 6000 treatment. Seedlings exposed to 15% PEG for 14 days exhibited a decrease in aboveground and underground dry mass, seedling height, root length, relative water content, photosynthetic pigment content, net photosynthetic rate （Pn）, transpiration rate （Tr）, stomatal conductance （Gs）, and water use efficiency. In PEG-stressed plants, the levels ofproline, malondialdehyde （MDA）, hydrogen peroxide （H2O2）, and electrolyte leakage rose significantly, whereas antioxidative activity, including superoxide, peroxidase, and catalase activities, declined in leaves. However, the presence of SA provided an effective method of mitigating PEG-caused physiological stresses on G. jasminoides seedlings, which depended on SA levels. PEG-treated plants exposed to SA at 0.5-1.0 mmol/L significantly eased PEG-induced growth inhibition. Application of SA, especially at concentrations of 0.5-1.0 mmol/L, considerably improved photosynthetic pigments, photosynthesis, antioxidative activity, relative water content, and proline accumulation, and decreased MDA content, H202 content, and electrolyte leakage. By contrast, the positive effects were not evident, or even more severe, in PEG＋SA4 treatment. Based on these physiological and biochemical data, a suitable concentration of SA, potential growth regulators, could be applied to enhance the drought tolerance of G. jasminoides.

HPLC法同时测定清脑止痛胶囊中原阿片碱和盐酸巴马汀含量

清脑止痛胶囊主要由白芍、川芎、葛根、延胡索、蒺藜、白芷、刺五加、夏天无八味药经过水蒸气蒸馏、水提、醇提三种工艺制备,具有祛风活血、止痛安神的功效。现有质量标准对君药白芍所含芍药苷进行了含量测定,并对葛根、夏天无、延胡索、刺五加的薄层进行了鉴别,方法稳定可行。白芍为水提药材,为进一步控制产品质量,本文采用高效液相色谱(HPLC)法同时测定清脑止痛胶囊中醇提部位夏天无中原阿片碱和盐酸巴马汀的含量,研究结果表明,本方法灵敏、快速、可靠,可用于清脑止痛胶囊的质量控制。

An information-based rough set approach to critical engineering factor identification

In order to analyze the main critical engineering factors, an information-based rough set approach that considers conditional information entropy as a measurement of information has been developed. An algorithm for continuous attribute discretization based on conditional information entropy and an algorithm for rule extraction considering the supports of rules are proposed. The initial decision system is established by collecting enough monitoring data. Then, the continuous attributes are discretized, and the condition attributes are reduced. Finally, the rules that indicate the action law of the main factors are extracted and the results are explained. By applying this approach to a crack in an arch gravity dam, it can be concluded that the water level and the temperature are the main factors affecting the crack opening, and there is a negative correlation between the crack opening and the temperature. This conclusion corresponds with the observation that cracks in most concrete dams are influenced mainly by water level and temperature, and the influence of temperature is more evident.

Comparative Study on Characteristics and Development of Chinese and Western Architectural Culture

Architecture is an indispensable part of the development of human civilization and the carrier of human civilization.The evolution of architectural style can also reflect the development of a nation.With the continuous development of history,the preservation and development of historic sites has gradually become a topic of common concern in China and the west.However,with the continuous development of modern technology,there are fewer and fewer traditional cultural elements in the newly-built buildings,which lead to the gradual loss of some traditional architectural culture.In order to avoid the further aggravation of this phenomenon,something should be done.From the perspective of the development characteristics of Chinese and western architectural culture,the characteristics and differences of the development process of Chinese and western architecture were listed from ancient to modern times,and gradually the main reasons for the different development directions and characteristics were analyzed.In addition,according to the law and characteristics of the development of Chinese and western architecture,some methods on how to solve the inheritance of Chinese modern architecture to traditional culture were put forward.

CDIO教育模式在测控专业实践教学中的应用——以视觉检测技术为例

本文以视觉检测技术课程教学为例，分析CDIO工程教育模式在测控专业实践教学中的应用，首先分析了视觉检测技术课程的特点，指出了觎觉检测技术课程采用CDIO工程实践教学的可行性，斗结合本系测控专业实践教学，给出了开展CDIO教育的具体应用案例。

Comparative Study on Characteristics and Development of Chinese and Western Architectural Culture

Architecture is an indispensable part of the development of human civilization and the carrier of human civilization.The evolution of architectural style can also reflect the development of a nation.With the continuous development of history,the preservation and development of historic sites has gradually become a topic of common concern in China and the west.However,with the continuous development of modern technology,there are fewer and fewer traditional cultural elements in the newly-built buildings,which lead to the gradual loss of some traditional architectural culture.In order to avoid the further aggravation of this phenomenon,something should be done.From the perspective of the development characteristics of Chinese and western architectural culture,the characteristics and differences of the development process of Chinese and western architecture were listed from ancient to modern times,and gradually the main reasons for the different development directions and characteristics were analyzed.In addition,according to the law and characteristics of the development of Chinese and western architecture,some methods on how to solve the inheritance of Chinese modern architecture to traditional culture were put forward.

Application of artificial intelligence in diagnosis of pulmonary tuberculosis

Tuberculosis(TB)is an infectious disease caused by Mycobacterium tuberculosis.Although the diagnostic technology of pulmonary tuberculosis(PTB)has advanced,accurate and differential diagnoses of PTB are still challenging.In recent years,the rapid development of artificial intelligence(AI)and its wide application in the medical field have provided new opportunities for diagnosing and treating TB and PTB.The machine learning model of AI has not only helped physicians improve diagnostic accuracy,but also enabled them to make early preventive diagnoses for individuals at increased risk of infection.Furthermore,AI can guide physicians to formulate targeted treatment strategies for PTB patients with different conditions.

CRISPR/Cas9-mediated editing of GmTAP1 confers enhanced resistance to Phytophthora sojae in soybean

Soybean(Glycine max) is an economically important oilbearing crop that is widely planted worldwide(Babu et al.,2017). Many diseases seriously limit soybean yield. Soybean root rot disease, one of the most destructive soybean diseases, occurs throughout the growth period, resulting in considerable yield losses(Kamoun et al., 2015).

基于中医传承计算平台(V3.0)探究中医药治疗儿童功能性便秘的用药规律

目的基于中医传承计算平台(V3.0)分析中医药治疗儿童功能性便秘(FC)的用药规律。方法收集2012年1月至2022年3月中国知网数据库、万方数据知识服务平台及维普数据库中有关中医药治疗儿童FC的处方,通过数据挖掘,分析药物频数、性味、归经及功效,并进行关联规则和聚类分析。结果共纳入文献134篇,处方140首,涉及中药156味,使用频次排名前10位的中药为枳实、火麻仁、甘草、白术、莱菔子、陈皮、厚朴、生地黄、山楂、鸡内金;药性以温、寒性中药使用频数最高,其次为平、凉、热性中药;药味以甘、苦味中药使用频数最高,其次为辛、酸、咸味中药;药物归经前3位是脾经、胃经、肺经;药物功效以补虚、消食及理气类中药为主;常用药物组合为枳实、火麻仁,枳实、厚朴,火麻仁、甘草,枳实、莱菔子及白术、陈皮;得到核心药物组合3组。结论中医药治疗儿童FC以补脾和胃、消食化积及润肠通便为主要原则,兼以清热理气等;用药以补虚、消食、理气为主,具有寒温并用、攻补兼施的特点,为中医药治疗儿童FC提供了新的思路。

CBX7 reprograms metabolic flux to protect against meningioma progression by modulating the USP44/c-MYC/LDHA axis

Meningioma is one of the most common primary neoplasms in the central nervous system,but no specific molecularly targeted therapy has been approved for the clinical treatment of aggressive meningiomas.There is hence an urgent demand to decrypt the biological and molecular landscape of malignant meningioma.Here,through the in-silica prescreening and 10-year follow-up studies of 445 meningioma patients,we uncovered that CBX7 expression progressively decreases with malignancy grade and neoplasia stage in meningioma,and a high CBX7 expression level predicts a favorable prognosis in meningioma patients.CBX7 restoration significantly induces cell cycle arrest and inhibits meningioma cell proliferation.iTRAQ-based proteomics analysis indicated that CBX7 restoration triggers the metabolic shift from glycolysis to oxidative phosphorylation.The mechanistic study demonstrated that CBX7 promotes the proteasome-dependent degradation of c-MYC protein by transcriptionally inhibiting the expression of a c-MYC deubiquitinase,USP44,consequently attenuates c-MYC-mediated transactivation of LDHA transcripts,and further inhibits glycolysis and subsequent cell proliferation.More importantly,the functional role of CBX7 was further confirmed in subcutaneous and orthotopic meningioma xenograft mouse models and meningioma patients.Altogether,our results shed light on the critical role of CBX7 in meningioma malignancy progression and identify the CBX7/USP44/c-MYC/LDHA axis as a promising therapeutic target against meningioma progression.

Urine biomarkers discovery by metabolomics and machine learning for Parkinson’s disease diagnoses

Parkinson’s disease(PD)is a complex neurological disorder that typically worsens with age.A wide range of pathologies makes PD a very heterogeneous condition,and there are currently no reliable diagnostic tests for this disease.The application of metabolomics to the study of PD has the potential to identify disease biomarkers through the systematic evaluation of metabolites.In this study,urine metabolic profiles of 215 urine samples from 104 PD patients and 111 healthy individuals were assessed based on liquid chromatography-mass spectrometry.The urine metabolic profile was first evaluated with partial leastsquares discriminant analysis,and then we integrated the metabolomic data with ensemble machine learning techniques using the voting strategy to achieve better predictive performance.A combination of 8-metabolite predictive panel performed well with an accuracy of over 90.7%.Compared to control subjects,PD patients had higher levels of 3-methoxytyramine,N-acetyl-l-tyrosine,orotic acid,uric acid,vanillic acid,and xanthine,and lower levels of 3,3-dimethylglutaric acid and imidazolelactic acid in their urine.The multi-metabolite prediction model developed in this study can serve as an initial point for future clinical studies.

Effect of hydrogen impurities on hydrogen oxidation activity of Pt/C catalyst in proton exchange membrane fuel cells

High-purity of hydrogen is vital to the guarantee of end usage in proton exchange membrane fuel cell(PEMFC)electric vehicles(EVs)with superior durability and low expense.However,the currently employed hydrogen,primarily from fossil fuel,still contains some poisoning impurities that significantly affect the durability of PEMFCs.Here,we investigate the poisoning effect of several typical hydrogen impurities(S^(2-),Cl^(-),HCOO^(-)and CO_(3)^(2-))on the hydrogen oxidation reaction(HOR)of the state-of-the-art carbon-supported platinum(Pt/C)catalyst used in the PEMFC anode.Electrochemical results indicate that the electrochemically active surface area of Pt/C is hampered by these hydrogen impurities with reduced effective Pt reactive sites due to the competitive adsorption against hydrogen at Pt sites showing the extent of the poisoning on Pt sites in the order:S^(2-)>Cl^(-)>HCOO^(-)>CO_(3)^(2-).Density functional theory calculations reveal that the adsorption energy of S2-on Pt(111)is greater than that of Cl^(-),HCOO^(-)and CO_(2),and the electronic structure of Pt is found to be changed due to the adsorption of impurities showing the downshift of the d-band centre of Pt that weakens the adsorption of hydrogen on the Pt sites.This work provides valuable guidance for future optimization of hydrogen quality and also emphasizes the importance of anti-poisoning anode catalyst development,especially towards H_(2)S impurities that seriously affect the durability of PEMFCs.

The Regulatory Effect of Braided Silk Fiber Skeletons with Differential Porosities on In Vivo Vascular Tissue Regeneration and Long-Term Patency

The development of small-diameter vascular grafts that can meet the long-term patency required for implementation in clinical practice presents a key challenge to the research field.Although techniques such as the braiding of scaffolds can offer a tunable platform for fabricating vascular grafts,the effects of braided silk fiber skeletons on the porosity,remodeling,and patency in vivo have not been thoroughly investigated.