Circ_0003855 involvement of esophageal cancer progression through miR-622/FLOT1

To confirm the relationship between Circ_0003855 and EC,we purchased the Human esophageal carcinoma cell line Eca109 and normal human esophageal epithelial cells HEEC,and the expression levels of Circ_0003855,miR-622,and FLOT1 were detected.The results show that Circ_0003855 and FLOT1 were highly expressed in Eca109 cells,while miR-622 was lowly expressed(p<0.05).Subsequently,Circ_0003855 small interfering RNA(si-Circ_0003855)and its negative control(si-NC)were used to detect changes in cellular biological behaviors.We found that the activity of Eca109 cells was reduced after interfering with the expression of Circ_0003855,and miR-622 expression was elevated,while FLOT1 was decreased(p<0.05).Additionally,si-Circ_0003855 and miR-622 inhibitor sequence(miR-622-inhibition)were co-transfected into cells with miR-622-inhibition alone,and untreated Eca109 cells were used as a control to detect the expression of FLOT1.Co-transfection of si-Circ_0003855 and miR-622-inhibition showed no significant difference in FLOT1 expression compared to the control cells(p>0.05).Synthesizing the results of these experiments above,we believe that interfering with the expression of Circ_0003855 can inhibit the activity of EC cells,and its mechanism is related to miR-622 and FLOT1.

Automatic SOC Equalization Strategy of Energy Storage Units with DC Microgrid Bus Voltage Support

In this paper,an improved sag control strategy based on automatic SOC equalization is proposed to solve the problems of slow SOC equalization and excessive bus voltage fluctuation amplitude and offset caused by load and PV power variations in a stand-alone DC microgrid.The strategy includes primary and secondary control.Among them,the primary control suppresses the DC microgrid voltage fluctuation through the I and II section control,and the secondary control aims to correct the P-U curve of the energy storage system and the PV system,thus reducing the steady-state bus voltage excursion.The simulation results demonstrate that the proposed control strategy effectively achieves SOC balancing and enhances the immunity of bus voltage.The proposed strategy improves the voltage fluctuation suppression ability by approximately 39.4%and 43.1%under the PV power and load power fluctuation conditions,respectively.Furthermore,the steady-state deviation of the bus voltage,△U_(dc) is only 0.01–0.1 V,ensuring stable operation of the DC microgrid in fluctuating power environments.

幼儿科学问题解决能力的发展特点与家长教育卷入的影响

为揭示幼儿科学问题解决能力的一般发展特点及家长教育卷入的影响,本研究运用幼儿科学问题解决能力测验与家长教育卷入问卷,对福建省5所幼儿园294名幼儿及其家长进行调查。结果表明,大班幼儿的科学问题解决能力总体高于中班幼儿,男生的科学问题解决能力总体高于女生;在难度较大的任务中,大班幼儿的表现显著优于中班幼儿,在难度较小的任务中,男生的表现显著优于女生;男生和女生的科学问题解决能力在4~4.5岁阶段均平稳缓慢发展;男生的科学问题解决能力在4.5~5.5岁阶段提升较为迅速;女生的科学问题解决能力在5~6岁阶段提升较为迅速。幼儿科学问题解决能力与家长教育卷入总分以及家长讨论、家长语言认知活动参与、家长生活指导等维度得分呈显著正相关。家长语言认知活动参与、家长生活指导对幼儿科学问题解决能力具有正向预测作用。家长应关注幼儿科学问题解决能力发展的年龄特点与性别差异,加强与幼儿的语言互动,培养幼儿生活自理能力,创设具有挑战性的科学探索环境,激发幼儿科学探索兴趣,以促进幼儿科学问题解决能力的发展。

Recovery of lithium using H_(4)Mn_(3.5)Ti_(1.5)O_(12)/reduced graphene oxide/polyacrylamide composite hydrogel from brine by Ads-ESIX process

Powdery Li^(+)-imprinted manganese oxides adsorbent was widely used to the recovery of Li^(+),but there are some difficulties,such as poor stability in acid solution,inconvenience of operation and separation.In this work,a useful hydrogel composite based H_(4)Mn_(3.5)Ti_(1.5)O_(12)/reduced graphene oxide/polyacrylamide(HMTO-rGO/PAM)was fabricated by thermal initiation method with promising stable,conductive and selective properties.The resulting materials were characterized by field emission scanning electron microscope,infrared absorption spectrum,X-ray diffraction,X-ray photoelectron spectroscopy and electrochemical techniques.The recovery of Li^(+)was investigated using HMTO-rGO/PAM from brine by a separated two-stage sorption statically and electrically switched ion exchange desorption process.The adsorption capacity of 51.5 mg·g^(-1)could be achieved with an initial Li^(+)concentration of 200 mg·L^(-1)in pH 10,at 45℃ for 12 h.Li^(+)ions could be quickly desorbed by cyclic voltammetry(CV)in pH 3,0.1 mol·L^(-1)HCl/NH;Cl accompanying the exchange of Li^(+)and H+(NH;)and the transfer of LMTO-rGO/PAM to HMTO-rGO/PAM.

Study on the Sustainable Development of the Tobacco-growing Area in South Anhui

Based on the actual situation of tobacco production in South Anhui tobacco-growing area,the paper analyzes several major constraints,and discusses several aspects such as tobacco production human resources,production of large-scale cultivation,science and technology service providers,the standardized production management and production security system. The countermeasures and suggestions for sustainable development are also put forward to provide a reference for the sustainable development of tobacco-growing area in South Anhui.

Engineering disease-resistant plants with alternative translation efficiency by switching uORF types through CRISPR

Engineering disease-resistant plants can be a powerful solution to the issue of food security.However,it requires addressing two fundamental questions:what genes to express and how to control their expressions.To find a solution,we screen CRISPR-edited upstream open reading frame(uORF)variants in rice,aiming to optimize translational control of disease-related genes.By switching uORF types of the 5′-leader from Arabidopsis TBF1,we modulate the ribosome accessibility to the downstream firefly luciferase.We assume that by switching uORF types using CRISPR,we could generate uORF variants with alternative translation efficiency(CRISPR-aTrE-uORF).These variants,capable of boosting translation for resistance-associated genes and dampening it for susceptible ones,can help pinpoint previously unidentified genes with optimal expression levels.To test the assumption,we screened edited uORF variants and found that enhanced translational suppression of the plastic glutamine synthetase 2 can provide broad-spectrum disease resistance in rice with minimal fitness costs.This strategy,which involves modifying uORFs from none to some,or from some to none or different ones,demonstrates how translational agriculture can speed up the development of disease-resistant crops.This is vital for tackling the food security challenges we face due to growing populations and changing climates.

The complexity of structural variations in Brassica rapa revealed by assembly of two complete T2T genomes

Brassica has long been of paramount importance to agriculture and human nutrition. Among the Brassica genus, Brassica rapa,the AA diploid, stands out as a prominent representative, valued for its versatility both as a vegetable and oil crop and its genetic proximity to other Brassica species. Economically, B. rapa plays an important role in the food industry, contributing to 12%of the global edible oil supply and 10%of vegetable yield [1].

Continuously released Zn^(2+)in 3D-printed PLGA/β-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties

Long-term nonunion of bone defects has always been a major problem in orthopedic treatment.Artificial bone graft materials such as Poly(lactic-co-glycolic acid)/β-tricalcium phosphate(PLGA/β-TCP)scaffolds are expected to solve this problem due to their suitable degradation rate and good osteoconductivity.However,insufficient mechanical properties,lack of osteoinductivity and infections after implanted limit its large-scale clinical application.Hence,we proposed a novel bone repair bioscaffold by adding zinc submicron particles to PLGA/β-TCP using low temperature rapid prototyping 3D printing technology.We first screened the scaffolds with 1 wt%Zn that had good biocompatibility and could stably release a safe dose of zinc ions within 16 weeks to ensure long-term non-toxicity.As designed,the scaffold had a multi-level porous structure of biomimetic cancellous bone,and the Young’s modulus(63.41±1.89 MPa)and compressive strength(2.887±0.025 MPa)of the scaffold were close to those of cancellous bone.In addition,after a series of in vitro and in vivo experiments,the scaffolds proved to have no adverse effects on the viability of BMSCs and promoted their adhesion and osteogenic differentiation,as well as exhibiting higher osteogenic and anti-inflammatory properties than PLGA/β-TCP scaffold without zinc particles.We also found that this osteogenic and anti-inflammatory effect might be related to Wnt/β-catenin,P38 MAPK and NFkB pathways.This study lay a foundation for the follow-up study of bone regeneration mechanism of Zn-containing biomaterials.We envision that this scaffold may become a new strategy for clinical treatment of bone defects.

Rational design of an AIE-active metal-organic framework for highly sensitive and portable sensing nitroaromatic explosives

Herein,we report a new metal-organic framework with an AIE ligand (H_(4)TCPP=2,3,5,6-tetra-(4-carboxyphenyl)pyrazine) and Mg^(2+) ions,that is,[Mg_(2)(H_(2)O)_(4)TCPP]·DMF·5CH_(3)CN (Mg-TCPP,TCPP=tetra-(4-carboxyphenyl)pyrazine) for detection of nitroaromatic explosives.Due to the coordination effect and restricted intramolecular rotation,Mg-TCPP exhibits bright blue light.As a fluorescent sensor,Mg-TCPP exhibits high selectivity and sensitivity for sensing 2,4,6-trinitrophenol (TNP) by quenching behaviors with the Stern-Volmer quenching constant (K_(SV)) of 3.63×10^(5)L/mol and achieves the low limit of detection of 25.6 ppb,which is beyond most of the previously reported fluorescent materials.Notably,the portable Mg-TCPP films are prepared and it can be used for rapid and sensitive TNP detection in a variety of environments including organic solvent and aqueous solution.Moreover,TNP vapor can be detected within 3 min by naked eye and the film could be regenerated under simple solvent cleaning.

Visualization of enantioselective recognition and separation of chiral acids by aggregation-induced emission chiral diamine

Enantioselective recognition and separation are the most important issues in the fields of chemistry,pharmacy,agrochemical,and food science.Here,we developed two optically active diamines showing aggregation-induced emission(AIE)that can discriminate 5 kinds of chiral acids with high enantioselectivity.Especially,a very high fluorescence intensity ratio(IL/ID)of 281 for(±)-Dibenzoyl-D/L-tartaric acid was obtained through the collection of fluorescence change after interaction with chiral AIE-active diamine.By virtue of AIE property and intermolecular acidbase interaction,enantioselective separation was facilely realized by simple filtration of the precipitates formed by chiral AIE luminogen(AIEgen)and one enantiomer in the racemic solution.The chiral HPLC data indicated that the precipitates of AIEgen/chiral acid possessed 82%L-analyte(the enantiomeric excess value was assessed to be 64%ee).Therefore,this method can serve as a simple,convenient,and low-cost tool for chiral detection and separation.

Triboelectrification induced UV emission from plasmon discharge

UV is a high-energy electromagnetic radiation that has been widely used in industrial production and the scientific research domain. In this work, a deep UV light emission was obtained using triboelectrification induced plasma discharge without any extra power supply. By a mechanical friction between polymer and quartz glass, the triboelectric charges cause a changing electric field, which may bring plasma discharge of low pressure gas （Ar-Hg） and give out 253.7 nm irradiation. The UV light caused by continuous friction can excite a trichromatic phosphor and afford a bright white light emission. A UV sterilization experiment shows that -98% of Escherichia coil can be killed in 30 min by UV irradiation, which reveals that a self-powered sterilization apparatus with good sterilization effect was fabricated. This work provides a novel design to fabricate a self- powered UV light emitting device using low-frequency mechanical friction and realizes the coupling of triboelectrificafion and plasma luminescence, which may further expand the application of UV light in special circumstances.

Microstructure, wear resistance, and corrosion performance of Ti35Zr28Nb alloy fabricated by powder metallurgy for orthopedic applications

A ternary Ti35Zr28Nb alloy was fabricated by powder metallurgy(PM)from pre-alloyed powder.The microstructure,hardness,corrosion behavior,and wear response of the produced alloy were investigated systematically.The results show that nearly full dense Ti35Zr28Nb alloy(relative density is 98.1±1.2%)can be fabricated by PM.The microstructure was dominated with uniform phase.The Ti35Zr28Nb alloy displayed spontaneous passivity in a naturally aerated simulated body fluid(SBF)solution at 37±0.5°C.The Ti35Zr28Nb alloy exhibited the highest corrosion resistance as compared to as-cast Ti6Al4V and pure Ti because of the formation of a protective passive film containing TiO2,Nb2O5,and ZrO2,including the highest corrosion potential(-0.22±0.01 V),the lowest corrosion current density(57.45±1.88 nA),the lowest passive potential(0.05±0.01 V)and the widest passivation range(1.29±0.09 V).Under the same wear condition,the wear rate of the Ti35Zr28Nb alloy(0.0021±0.0002 mm3/m·N)was lower than that of the CP Ti(0.0029±0.0004 mm3/m·N)and close to that of the Ti6Al4V(0.0020±0.0003 mm3/m·N).The wear mechanism of the Ti35Zr28Nb alloy was mainly dominated by abrasive wear,accompanied by adhesive wear.The highest corrosion resistance together with the adequate wear resistance makes the PM-fabricated Ti35Zr28Nb alloy an attractive candidate for orthopedic implant materials.

miR395-regulated sulfate metabolism exploits pathogen sensitivity to sulfate to boost immunity in rice

MicroRNAs (miRNAs) play important roles in plant physiological activities. However, their roles and molecular mechanisms in boosting plant immunity, especially through the modulation of macronutrient metabolism in response to pathogens, are largely unknown. Here, we report that an evolutionarily conserved miRNA, miR395, promotes resistance to Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), two destructive bacterial pathogens, by regulating sulfate accumulation and distribution in rice. Specifically, miR395 targets and suppresses the expression of the ATP sulfurylase gene OsAPS1, which functions in sulfate assimilation, and two sulfate transporter genes, OsSULTR2;1 and OsSULTR2;2, which function in sulfate translocation, to promote sulfate accumulation, resulting in broad-spectrum resistance to bacterial pathogens in miR395-overexpressing plants. Genetic analysis revealed that miR395-triggered resistance is involved in both pathogen-associated molecular pattern-triggered immunity and R gene-mediated resistance. Moreover, we found that accumulated sulfate but not S-metabolites inhibits proliferation of pathogenic bacteria, revealing a sulfate-mediated antibacterial defense mechanism that differs from sulfur-induced resistance. Furthermore, compared with other bacteria, Xoo and Xoc, which lack the sulfate transporter CysZ, are sensitive to high levels of extracellular sulfate. Accordingly, miR395-regulated sulfate accumulation impaired the virulence of Xoo and Xoc by decreasing extracellular polysaccharide production and biofilm formation. Taken together, these results suggest that rice miR395 modulates sulfate metabolism to exploit pathogen sensitivity to sulfate and thereby promotes broad-spectrum resistance.

A hydrogel spinal dural patch with potential anti-inflammatory,pain relieving and antibacterial effects

CSFL caused by spinal dural defect is a common complication of spinal surgery,which need repair such as suture or sealants.However,low intracranial pressure symptoms,wound infection and prolonged hospital associated with pin-hole leakage or loose seal effect were often occurred after surgical suture or sealants repair.Stable,pressure resistance and high viscosity spinal dural repair patch in wet environment without suture or sealants was highly needed.Herein,a bioactive patch composed of alginate and polyacrylamide hydrogel matrix cross-linked by calcium ions,and chitosan adhesive was proposed.This fabricated patch exhibits the capabilities of promoting defect closure and good tight seal ability with the bursting pressure is more than 790 mm H2O in wet environment.In addition,the chitosan adhesive layer of the patch could inhibit the growth of bacterial in vitro,which is meaningful for the postoperative infection.Furthermore,the patch also significantly reduced the expression of GFAP,IBA-1,MBP,TNF-α,and COX-2 in early postoperative period in vivo study,exerting the effects of anti-inflammatory,analgesic and adhesion prevention.Thus,the bioactive patch expected to be applied in spinal dural repair with the good properties of withstanding high pressure,promoting defect closure and inhibiting postoperative infection.

Precise Editing Enables Crop Broad-Spectrum Resistance

Loss of resistance to fast-adapted pathogens urgently calls for a sustainable and broad-spectrum strategy to meet the requirement of crop yield and safety for the increasing world population.Pyramidization of resistance genes,such as nucleotide-binding leucine-rich repeat proteins,from diverse cultivars,landraces,and domesticated or wild germplasm accessions offers the most direct practice for this purpose.Nevertheless,the facts of a narrow resistance spectrum and limited number or even shortage of such resistance genes to many specific pathogens leave an immediate question to be resolved in such a situation.In light of the evidence that all compatible pathogens trigger a conserved and wide range of host genes,namely susceptibility genes for their proliferation,management of those genes are of equal importance and promising for improvement in crop disease resistance(Zaidi et al.,2018).

Effects of Ti content on dielectric and energy storage properties of(Pb_(0.94)La_(0.04))[(Zr_(0.70)Sn_(0.30))_(1-x)Ti_(x)]O_(3)ferroelectric/antiferroelectric ceramics

Ferroelectric/antiferroelectric(FE/AFE)ceramics with composition of(Pb_(0.94)La_(0.04))[(Zr_(0.70)Sn_(0.30))_(1-x)Ti_(x)]O_(3)were fabricated via solid state reaction and the effects of Ti content on dielectric and energy storage properties were studied.High releasable energy density of 1.03 J/cm^(3)was obtained when x=0:12 under 65.7 kV/cm.With increasing Ti content,both the forward and backward phase transition fields would decrease.When x=0.16,the dielectrics would be under FE state at room temperature(22℃)and the phase transition from FE to AFE and then to paraelectric(PE)was observed with temperature rise.The discharge properties were also studied and the results proved that the stored charge in AFE could be released much more completely than that in FE.

Porous Ti-10Mo alloy fabricated by powder metallurgy for promoting bone regeneration

Porous Ti-lOMo alloys were fabricated by powder metallurgy using a space-holder method. The pore characteristics, m icrostructure, mechanical properties, in vitro biocompatibility, and in vivo osseointegration of the fabricated alloys were systematically investigated. The results show that with different weight ratios of the space-holder (NH4- HC03) added, all of the porous Ti-10Mo alloys sintered at 1,300℃ exhibited a typical W idmanstatten microstructure. The porosity and average pore size of the porous structures can be controlled in the range of 50.8%-66.9% and 70.1 -381.4μm , respectively. The Ti-10Mo alloy with 63.4% porosity exhibited the most suitable mechanical properties for implant applications with an elastic modulus of 2.9 GPa and a compressive yield strength of 127.5 MPa. In vitro9 the alloyconditioned medium showed no deleterious effect on the cell proliferation. The cell viability in this medium was higher than that of the reference group, suggesting non-toxicity and good biological characteristics of the alloy specimens. In vivo, after eight weeks* implantation, new bone tissue formed surrounding the alloy implants, and no noticeable inflammation was observed at the implantation site. The bone bonding strength of the porous Ti-10Mo alloy increased over time from 46.6N at two weeks to 176.4 N at eight weeks. Suitable mechanical properties together with excellent biocompatibility in vitro and osteointegration in vivo make the porous Ti-10Mo fabricated by powder metallurgy an attractive orthopedic implant alloy.

A new digital information storing and reading system based on synthetic DNA

Dear Editor,In the＂big data era＂,the amount of digital information is growing explosively,therefore,a reliable data storage medium for large-scale digital archiving is urgently needed.However,the increase of existing storage capacity cannot keep up with the growth of digital information.Moreover,the durability of conventional storage teclanologles, sucn as magnetic and optical devices, is very limited. Since the first demonstration of using DNA to store messages in 1988, DNA has been considered as a promising data storage medium due to its high-density and long-term stability （half-life〉500years） （Allentoft et al., 2012）.