Some of the common practices to assess the composition of plant extract,including Carica papaya seed extract(CPSE)are direct injection of the extract,compound separation using polyethylene glycol capillary column(DB-W...Some of the common practices to assess the composition of plant extract,including Carica papaya seed extract(CPSE)are direct injection of the extract,compound separation using polyethylene glycol capillary column(DB-WAX),and non-linear-temperature programming(NLTP)of GC/MS analysis.This study specifically compared the coating of capillary column,sample derivatization,and temperature programming of GC/MS to determine the composition of CPSE.The retention indices(RI)of the detected compounds were determined and compared to the reference RI.In particular,5%phenyl-95%methylpolysiloxane(HP-5MS)-,DB-WAX-,and biscyanopropyl polysiloxane(HP-88)-coated capillary columns were used to identify the composition of CPSE.For this study,HP-5MS column,which separated the highest number of compounds(26 compounds)from CPSE,was deemed as the most suitable column.The GC/MS analysis of derivatized CPSE identified 21 compound groups,where fatty acids and fatty acid methyl esters served as the major compounds(80.23%),followed by these compounds in decreasing order:amides>nitriles>sterols>fatty aldehydes>organic acids.A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming(LTP)(R^(2)=0.9859)was found,as compared to its correlation determination with NLTP(R^(2)=0.9175),which exhibited an almost equal RI of LTP to the reference RI.Conclusively,GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended.展开更多
基金This work was supported by the Malaysia Fundamental Research Grant Scheme(FRGS19-041-0649 or FRGS/1/2018/STG04/UIAM/03/1)of Ministry of Higher Education Malaysiathe Research University Grant(vote number 9328200)of Universiti Putra Malaysia.
文摘Some of the common practices to assess the composition of plant extract,including Carica papaya seed extract(CPSE)are direct injection of the extract,compound separation using polyethylene glycol capillary column(DB-WAX),and non-linear-temperature programming(NLTP)of GC/MS analysis.This study specifically compared the coating of capillary column,sample derivatization,and temperature programming of GC/MS to determine the composition of CPSE.The retention indices(RI)of the detected compounds were determined and compared to the reference RI.In particular,5%phenyl-95%methylpolysiloxane(HP-5MS)-,DB-WAX-,and biscyanopropyl polysiloxane(HP-88)-coated capillary columns were used to identify the composition of CPSE.For this study,HP-5MS column,which separated the highest number of compounds(26 compounds)from CPSE,was deemed as the most suitable column.The GC/MS analysis of derivatized CPSE identified 21 compound groups,where fatty acids and fatty acid methyl esters served as the major compounds(80.23%),followed by these compounds in decreasing order:amides>nitriles>sterols>fatty aldehydes>organic acids.A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming(LTP)(R^(2)=0.9859)was found,as compared to its correlation determination with NLTP(R^(2)=0.9175),which exhibited an almost equal RI of LTP to the reference RI.Conclusively,GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended.
