Although optical microscopy is a widely used technique across various multidisciplinary fields for inspecting small-scale objects,surfaces or organisms,it faces a significant limitation:the lateral resolution of optic...Although optical microscopy is a widely used technique across various multidisciplinary fields for inspecting small-scale objects,surfaces or organisms,it faces a significant limitation:the lateral resolution of optical microscopes is fundamentally constrained by light diffraction.Dielectric micro-spheres,however,offer a promising solution to this issue as they are capable of significantly enhancing lateral resolution through extraordinary phenomena,such as a photonic nanojet.Building upon the potential of dielectric micro-spheres,this paper introduces a novel approach for fabricating 3D micro-devices designed to enhance lateral resolution in optical microscopy.The proposed 3D micro-device comprises a modified coverslip and a micro-sphere,facilitating easy handling and integration into any existing optical microscope.To manufacture the device,two advanced femtosecond laser techniques are employed:femtosecond laser ablation and multi-photon lithography.Femtosecond laser ablation was employed to create a micro-hole in the coverslip,which allows light to be focused through this aperture.Multi-photon lithography was used to fabricate a micro-sphere with a diameter of 20μm,along with a cantilever that positions the above the processed micro-hole and connect it with the coverslip.In this context,advanced processing strategies for multi-photon lithography to produce a micro-sphere with superior surface roughness and almost perfect geometry(λ/8)from a Zr-based hybrid photoresist are demonstrated.The performance of the micro-device was evaluated using Mirau-type coherence scanning interferometry in conjunction with white light illumination at a central wavelength of 600 nm and a calibration grid(Λ=0.28μm,h>50 nm).Here,the 3D micro-device proved to be capable of enhancing lateral resolution beyond the limits achievable with conventional lenses or microscope objectives when used in air.Simultaneously,it maintained the high axial resolution characteristic of Mirau-type coherence scanning interferometry.The results and optical properties of the micro-sphere were analyzed and further discussed through simulations.展开更多
基金supported by the Marie Skłodowska-Curie Actions,under grant agreement No.101059253,as part of the European Union’s Horizon Europe research and innovation programmeIt also received support from a Feodor Lynen Postdoctoral Fellowship awarded by the Alexander von Humboldt Foundation.Additional funding was provided by Laserlab-Europe(Proposal IDs:ULF-FORTH_002794 and ULF-FORTH_025264).We further gratefully acknowledge funding by the German Federal Ministry for Economic Affairs and Climate Action under grant 16KN053050.The authors would also like to thank Mrs.Aleka Manousaki for SEM technical support.
文摘Although optical microscopy is a widely used technique across various multidisciplinary fields for inspecting small-scale objects,surfaces or organisms,it faces a significant limitation:the lateral resolution of optical microscopes is fundamentally constrained by light diffraction.Dielectric micro-spheres,however,offer a promising solution to this issue as they are capable of significantly enhancing lateral resolution through extraordinary phenomena,such as a photonic nanojet.Building upon the potential of dielectric micro-spheres,this paper introduces a novel approach for fabricating 3D micro-devices designed to enhance lateral resolution in optical microscopy.The proposed 3D micro-device comprises a modified coverslip and a micro-sphere,facilitating easy handling and integration into any existing optical microscope.To manufacture the device,two advanced femtosecond laser techniques are employed:femtosecond laser ablation and multi-photon lithography.Femtosecond laser ablation was employed to create a micro-hole in the coverslip,which allows light to be focused through this aperture.Multi-photon lithography was used to fabricate a micro-sphere with a diameter of 20μm,along with a cantilever that positions the above the processed micro-hole and connect it with the coverslip.In this context,advanced processing strategies for multi-photon lithography to produce a micro-sphere with superior surface roughness and almost perfect geometry(λ/8)from a Zr-based hybrid photoresist are demonstrated.The performance of the micro-device was evaluated using Mirau-type coherence scanning interferometry in conjunction with white light illumination at a central wavelength of 600 nm and a calibration grid(Λ=0.28μm,h>50 nm).Here,the 3D micro-device proved to be capable of enhancing lateral resolution beyond the limits achievable with conventional lenses or microscope objectives when used in air.Simultaneously,it maintained the high axial resolution characteristic of Mirau-type coherence scanning interferometry.The results and optical properties of the micro-sphere were analyzed and further discussed through simulations.
