Broad and safe access to ultrafast laser technology has been hindered by the absence of optical fiber-delivered pulses with tunable central wavelength,pulse repetition rate,and pulse width in the picosecond–femtoseco...Broad and safe access to ultrafast laser technology has been hindered by the absence of optical fiber-delivered pulses with tunable central wavelength,pulse repetition rate,and pulse width in the picosecond–femtosecond regime.To address this long-standing obstacle,we developed a reliable accessory for femtosecond ytterbium fiber chirped pulse amplifiers,termed a fiber-optic nonlinear wavelength converter(FNWC),as an adaptive optical source for the emergent field of femtosecond biophotonics.This accessory empowers the fixed-wavelength laser to produce fiber-delivered∼20 nJ pulses with central wavelength across 950 to 1150 nm,repetition rate across 1 to 10 MHz,and pulse width across 40 to 400 fs,with a long-term stability of>2000 h.As a prototypical label-free application in biology and medicine,we demonstrate the utility of FNWC in real-time intravital imaging synergistically integrated with modern machine learning and largescale fluorescence lifetime imaging microscopy.展开更多
基金support from the National Institutes of Health,U.S.Department of Health and Human Services(Grant No.R01 CA241618)J.E.S.and R.R.I were supported by NIBIB/NIH(Award No.T32EB019944).
文摘Broad and safe access to ultrafast laser technology has been hindered by the absence of optical fiber-delivered pulses with tunable central wavelength,pulse repetition rate,and pulse width in the picosecond–femtosecond regime.To address this long-standing obstacle,we developed a reliable accessory for femtosecond ytterbium fiber chirped pulse amplifiers,termed a fiber-optic nonlinear wavelength converter(FNWC),as an adaptive optical source for the emergent field of femtosecond biophotonics.This accessory empowers the fixed-wavelength laser to produce fiber-delivered∼20 nJ pulses with central wavelength across 950 to 1150 nm,repetition rate across 1 to 10 MHz,and pulse width across 40 to 400 fs,with a long-term stability of>2000 h.As a prototypical label-free application in biology and medicine,we demonstrate the utility of FNWC in real-time intravital imaging synergistically integrated with modern machine learning and largescale fluorescence lifetime imaging microscopy.
