Effect of melatonin on the severity of L-arginine-induced experimental acute pancreatitis in rats

瞄准：决定 melatonin 预告的处理的效果并且在 L 精氨酸(L-Arg ) 的严厉上处理以后在老鼠的导致的试验性的胰腺炎。方法：男 Wistar 老鼠(25 ) 被划分成五个组。那些在 1h 的间隔在组 A 收到了 3.2g/kg 体重 L-Arg i.p 的二注射。在组麻省，老鼠与 50 mg/kg 体重 melatonin i.p 被对待在 L-Arg 管理以前的 30 min。在组 AM，在 L-Arg 被给以后，老鼠收到了 melatonin 1h 的一样的剂量。在组 M， melatonin 的单次量是以前描述了被管理。在组 C，对照动物收到了生理盐水注射 i.p 所有老鼠被给在第二 L-Arg 注射以后的 24 h。结果：引起严重引起坏死胰腺炎的 L-Arg 管理在浆液淀粉酶水平，胰腺的重量 / 身体重量比率(pw/bw ) ，胰腺的 IL-6 内容和 myeloperoxidase 由重要举起证实了活动，相对控制值。浆液淀粉酶水平的举起显著地在与仅仅与 L-Arg 注射的老鼠相比给 melatonin 追随者 L-Arg 的老鼠被减少。胰腺的抗氧化剂酶(Cu/Zn-superoxide 歧化酶(Cu/Zn-SOD ) 和过氧化氢酶(猫)) 的活动显著地被增加在胰腺炎以后的 24 h 感应。显著地在 L-Arg 之前给的 Melatonin 在独自与 L-Arg 对待的老鼠相对那减少了胰腺的猫活动。在肝， L-Arg 显著地每氧化水平，和谷胱甘肽过氧化物酶和 Cu/Zn-SOD 活动增加了类脂化合物，而 Mn 草皮活动作为与控制老鼠相比被减少。Melatonin 预告的处理阻止了这些变化。结论：Melatonin 是能在尖锐胰腺炎期间抵抗一些 L-Arg-induced 变化的一种抗氧化剂，并且可能因此在有尖锐引起坏死胰腺炎的病人的支持的治疗有用。

A case of interstitial pneumonitis in a patient with ulcerative colitis treated with azathioprine

The early hypersensitivity reaction and late bone marrow depression are well-known side-effects of azathioprine, whereas interstitial pneumonia is a rare complication. A 40-year old male patient had been treated with azathioprine in consequence of extensive ulcerative colitis for 10 years. He then complained of 7 d of fever, cough and catarrhal signs, without symptoms of active colitis. Opportunistic infections were ruled out. The chest X-ray, CT and lung biopsy demonstrated the presence of interstitial inflammation. Azathioprine therapy was discontinued as a potential source of the pulmonary infiltrate. In response to steroid therapy, and intensive care, the pulmonary infiltrate gradually decreased within 4 wk. Three months later, his ulcerative colitis relapsed, and ileo-anal pouch surgery was performed. In cases of atypical pneumonia, without a proven infection, azathioprine-associated interstitial pneumonitis may be present, which heals after withdrawal of the drug.

NOD2/CARD15 gene polymorphism in patients with inflammatory bowel disease: Is Hungary different?

AIM: To analyse the impact of NOD2/CARD15 mutations on the clinical course of Crohn's disease patients from an eastern European country (Hungary).METHODS: We investigated the prevalence of the three common NOD2/CARD15 mutations (Arg702Trp, Gly908Arg,1007finsC) in 148 patients with Crohn's disease, 128patients with ulcerative colitis and 208 controls recruited from the University of Szeged, Hungary. In patients with Crohn's disease, the prevalence of NOD2/CARD15 mutations was correlated to the demographical and clinical parameters.RESULTS: In total, 32.4% of Crohn's disease patients carried at least one mutant allele within NOD2/CARD15compared to 13.2% of patients with ulcerative colitis (P = 0.0002) and to 11.5% of controls (P＜0.0001). In Crohn's disease patients, the allele frequencies for Arg702Trp,Gly908Arg and 1007finsC were 7.1%, 3.0% and 10.8%respectively. Interestingly, only the 1007finsC mutation was associated with a distinct clinical phenotype. The patients positive for the 1007finsC mutation suffered more frequently from stenotic disease behaviour (P = 0.008). Furthermore,51.9% of patients positive for the 1007finsC mutation underwent a surgical resection within the ileum compared to only 17.4% of patients without the 1007finsC mutation (P = 0.001). With respect to the other two mutations (Arg702Trp and Gly908Arg), no associations were found with all investigated clinical parameters.CONCLUSION: NOD2/CARD15 mutations are frequently found in Crohn's disease patients from Hungary. The 1007finsC mutation is associated with stenotic disease behaviour and frequent ileal resections.