Objective:To isolate and characterize the saponin from Persian Gulf brittle star(Ophiocoma erinaceus)and to evaluate its hemolytic and cytotoxic potential. Methods:In an attempt to prepare saponin from brittle star,co...Objective:To isolate and characterize the saponin from Persian Gulf brittle star(Ophiocoma erinaceus)and to evaluate its hemolytic and cytotoxic potential. Methods:In an attempt to prepare saponin from brittle star,collected samples were minced and extracted with ethanol,dichloromethane,n-buthanol.Then,concentrated n-butanol extract were loaded on HP-20 resin and washed with dionized water,80%ethanol and 100%ethanol respectively.Subsequently,detection of saponin was performed by foaming property,fourier transform infrared spectroscopy and hemolytic analysis on thin layer chromatography.The cytotoxic activity on HeLa cells was evaluated through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide(MTT)assay and under invert microscopy.Results:The existence of saponin in Ophiocoma erinaceus were approved by phytochemical method.The presence of C-H bond,C-O-C and OH in fourier transform infrared spectrum of fraction 80%ethanol is characteristic feature in the many of saponin compounds.Hemolytic assay revealed HD_(50) value was 500μg/mL.MTT assay exhibited that saponin extracted in IC_(50) value of 25μg/mL inducsd potent cytotoxic activity against HeLa cells in 24 h and 12.5μg/mL in 48 h,meanwhile in lower concentration did not have considerable effect against HeLa cells.Conclusions:These findings showed that only 80%ethanol fraction Persian Gulf brittle star contained saponin like compounds with hemolytic activity which can be detected simply by phytochemical that can be appreciable for future anticancer research.展开更多
文摘Objective:To isolate and characterize the saponin from Persian Gulf brittle star(Ophiocoma erinaceus)and to evaluate its hemolytic and cytotoxic potential. Methods:In an attempt to prepare saponin from brittle star,collected samples were minced and extracted with ethanol,dichloromethane,n-buthanol.Then,concentrated n-butanol extract were loaded on HP-20 resin and washed with dionized water,80%ethanol and 100%ethanol respectively.Subsequently,detection of saponin was performed by foaming property,fourier transform infrared spectroscopy and hemolytic analysis on thin layer chromatography.The cytotoxic activity on HeLa cells was evaluated through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide(MTT)assay and under invert microscopy.Results:The existence of saponin in Ophiocoma erinaceus were approved by phytochemical method.The presence of C-H bond,C-O-C and OH in fourier transform infrared spectrum of fraction 80%ethanol is characteristic feature in the many of saponin compounds.Hemolytic assay revealed HD_(50) value was 500μg/mL.MTT assay exhibited that saponin extracted in IC_(50) value of 25μg/mL inducsd potent cytotoxic activity against HeLa cells in 24 h and 12.5μg/mL in 48 h,meanwhile in lower concentration did not have considerable effect against HeLa cells.Conclusions:These findings showed that only 80%ethanol fraction Persian Gulf brittle star contained saponin like compounds with hemolytic activity which can be detected simply by phytochemical that can be appreciable for future anticancer research.
