Antioxidant and antipyretic properties of methanolic extract of Amaranthus spinosus leaves

Objective:Methanolic extract of Amaranthus spinosus（A.spinosus） leaves was screened for antioxidant and antipyretic activities.Methods:Antioxidant activity was measured by l,l-diphenyl-2-picryl-hydrazile（DPPH） free radical scavenging,superoxide anion radical scavenging,hydroxyl free radical scavenging,nitric oxide radical scavenging,2,2 -azinobis-3- ethylbenzothiazole-6-sulfonic acid（ABTS） radical scavenging assays and total phenolic content was also determined.Antipyretic activity of methanolic extract of A.spinosus was measured by yeast induced pyrexia method at concentration of 200 and 400 mg/kg using paracetamol as standard drug.Results:Methanolic extract of A.spinosus showed potent antioxidant activity.The IC50 value was（87.50±3.52）μg/mL,（98.80±1.40）μg/mL,（106.25±0.20）μg/mL,（88.70±0.62）μg/mL and（147.50±2.61）μg/mL for DPPH,superoxide,hydroxyl,nitric oxide and ABTS radical scavenging activities.Methanolic extract of A spinosus showed significant（P【0.01） antipyretic activity.

Pain management in mice using methanol extracts of three plants belongs to family Amaranthaceae

Objective:To investigate the analgesic activity of methanolic extract of Amaranthus viridis(A. viridis),Amaranthus caudatus(A.caudatus) and Amaranthus spinosus(A.spinosus).Methods:In this study,the analgesic activity of methanol extracts of all three plants at doses of 200 and 400 mg/kg were investigated by acetic acid-induced writhings test,hot plate test and tail immersion test for mice.Results:It was found that all the three plants showed significant pain management effect(P【0.01) at a dose of 400 mg/kg,but showed a less significant effect at a dose of 20 mg/kg in the entire tests used for evaluation of analgesic activities(P【0.05).Conclusions:Methanol extracts of A.viridis,A.caudatus and A.spinosus show potent analgesic activities,and this study provides the scientific proof for their traditional claims.