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犬体内细粒棘球绦虫和多房棘球绦虫的混合感染 被引量:19
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作者 温浩 张亚楼 +6 位作者 jean-mathieu bart Giraudoux P Vuitton DA 马旭东 邹林樾 苗玉清 Craig PS 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2006年第1期10-13,共4页
目的 证实家犬体内是否存在细粒棘球绦虫和多房棘球绦虫混合感染。方法 从新疆和静县巴音布鲁克草原现场收购的30条牧羊犬,经麻醉后处死解剖,在1只雌性牧羊犬小肠内发现棘球绦虫成虫1万条以上,经显微镜观察,疑为细粒棘球绦虫和多房... 目的 证实家犬体内是否存在细粒棘球绦虫和多房棘球绦虫混合感染。方法 从新疆和静县巴音布鲁克草原现场收购的30条牧羊犬,经麻醉后处死解剖,在1只雌性牧羊犬小肠内发现棘球绦虫成虫1万条以上,经显微镜观察,疑为细粒棘球绦虫和多房棘球绦虫混合感染。用Eglf/r和EM-15/17EM引物分别对两种棘球绦虫的线粒体DNA特异目的片段进行序列分析鉴定。结果 形态学观察:细粒棘球绦虫孕节长大,生殖孔偏后,位于节片一侧中部。子宫有不规则的分支和侧突(侧囊),内含虫卵200~800个。多房棘球绦虫较短小,4~5体节。孕节中子宫呈简单的囊状,无侧囊。生殖孔开口于侧缘的前半部。线粒体12S RNA序列鉴定,扩增样本DNA经同源序列比较发现,与细粒棘球绦虫G1型具有相同的序列;扩增样本与多房棘球绦虫具有相同的序列。分别确定为细粒棘球绦虫和多房棘球绦虫两种虫种。结论 首次证实家犬体内存在细粒棘球绦虫和多房棘球绦虫的混合感染。 展开更多
关键词 细粒棘球绦虫 多房棘球绦虫 混合感染 家犬 PCR鉴定
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新疆家犬体内存在细粒棘球绦虫G1(羊)株和G6(骆驼)株的分子证据(英文) 被引量:8
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作者 张亚楼 jean-mathieu bart +5 位作者 温浩 马旭东 苗玉清 林仁勇 王星 卢晓梅 《中国寄生虫病防治杂志》 CSCD 2005年第5期333-335,共3页
目的收集家犬体内的细粒棘球绦虫成虫以建立新疆棘球蚴病的分子流行病学资料。方法对家犬体内成虫细胞色素c氧化酶Ⅰ基因序列进行测定以确定其亚株型。结果所有感染犬体内成虫的基因型为G1型。特别是1条家犬体内发现存在细粒棘球绦虫G1(... 目的收集家犬体内的细粒棘球绦虫成虫以建立新疆棘球蚴病的分子流行病学资料。方法对家犬体内成虫细胞色素c氧化酶Ⅰ基因序列进行测定以确定其亚株型。结果所有感染犬体内成虫的基因型为G1型。特别是1条家犬体内发现存在细粒棘球绦虫G1(羊)株和G6(骆驼)株混合感染的情况。结论在新疆阻断羊犬和羊骆驼循环圈是预防棘球蚴病的重要措施。作为终末宿主的家犬与人类的感染密切相关,对感染犬的管理应该加强。 展开更多
关键词 细粒棘球绦虫 细胞色素c氧化酶Ⅰ基因 棘球蚴病
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Specificity of serological screening tests and reference laboratory tests to diagnose gambiense human African trypanosomiasis: a prospective clinical performance study
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作者 Martial Kassi N'Djetchil Oumou Camara +25 位作者 Mathurin Koffi Mamadou Camara Dramane Kaba Jacques Kabore Alkali Tall Brice Rotureau Lucy Glover Melika Barkissa Traorel Minayegninrin Kone Bamoro Coulibaly Guy Pacome Adingra Aissata Soumah Mohamed Gassama Abdoulaye Dansy Camara Charlie Franck Alfred Compaore Aissata Camara Salimatou Boiro Elena Perez Anton Paul Bessell Nick Van Reet Bruno Bucheton Vincent Jamonneau jean-mathieu bart Philippe Solano Sylvain Bieler Veerle Lejon 《Infectious Diseases of Poverty》 SCIE CAS CSCD 2024年第4期48-63,共16页
Background Serological screening tests play a crucial role to diagnose gambiense human African trypanosomiasis(gHAT).Presently,they preselect individuals for microscopic confrmation,but in future"screen and treat... Background Serological screening tests play a crucial role to diagnose gambiense human African trypanosomiasis(gHAT).Presently,they preselect individuals for microscopic confrmation,but in future"screen and treat"strategies they willidentify individuals for treatment.Variability in reported specificities,the development of new rapid diagnos-tic tests(RDT)and the hypothesis that malaria infection may decrease RDT specificity led us to evaluate the specificity of 5 gHAT screening tests.Methods During active screening,venous blood samples from 1095 individuals from Cote d'ivoire and Guinea were tested consecutively with commercial(CATT,HAT Sero-K-SeT,Abbott Bioline HAT 2.0)and prototype(DCN HAT RDT,HAT Sero-K-SeT 2.0)gHAT screening tests and with a malaria RDT.Individuals with≥1 positive gHAT screening test underwent microscopy and further immunological(trypanolysis with T.b.gambiense LiTat 1.3,1.5 and 1.6;indirect ELISA/Tb.gambiense;T.b.gambiense inhibition ELISA with T.b.gambiense LiTat 1.3 and 1.5 VSG)and molecular reference laboratory tests(PCR TBRN3,18S and TgsGP;SHERLOCK 18S Tids,7SL Zoon,and TgsGP;Trypanozoon S2-RT-qPCR 18S2,177T,GPl-PLC and TgsGP in multiplex;RT-qPCR DT8,DT9 and TgsGP in multiplex).Microscopic trypanosome detection confrmed gHAT,while other individuals were considered gHAT free.Differences in fractions between groups were assessed by Chi square and differences in specificity between 2 tests on the same individuals by McNemar.Results One gHAT case was diagnosed.Overall test specificities(n=1094)were:CATT 98.9%(95%CI:98.1-99.4%);HAT Sero-K-SeT 86.7%(95%CI:84.5-88.5%);Bioline HAT 2.082.1%(95%CI:79.7-84.2%);DCN HAT RDT 78.2%(95%CI:75.7-80.6%);and HAT Sero-K-SeT 2.078.4%(95%CI:75.9-80.8%).In malaria positives,gHAT screening tests appeared less specific,but the difference Was significant only in Guinea for Abbott Bioline HAT 2.0(P=0.03)and HAT Sero-K-Set 2.0(P=0.0006).The specificities of immunological and molecular laboratory tests in gHAT seropositives were 98.7-100%(n=399)and 93.0-100%(n=302),respectively.Among 44 reference laboratory test positives,only the confrmed gHAT patient and one screening test seropositive combined immunological and molecular reference laboratory test positivity.Conclusions Although a minor effect of malaria cannot be excluded,gHAT RDT specificities are far below the 95%minimal specificity stipulated by the WHO target product profile for a simple diagnostic tool to identify individuals eligible for treatment.Unless specificity is improved,an RDT-based"screen and treat"strategy would result in massive overtreatment.In view of their inconsistent results,additional comparative evaluations of the diagnostic performance of reference laboratory tests are indicated for better identifying,among screening test positives,those at increased suspicion for gHAT. 展开更多
关键词 Human African trypanosomiasis Trypanosoma brucei gambiense Diagnosis SPECIFICITY Rapid diagnostic test Immunoloaical test Molecular test
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