Mass production of black soldier fly,Hermetia illucens(L.)(Diptera:Stra-tiomyidae),larvae results in massive heat generation,which impacts facility management,waste conversion,and larval production.We tested daily sub...Mass production of black soldier fly,Hermetia illucens(L.)(Diptera:Stra-tiomyidae),larvae results in massive heat generation,which impacts facility management,waste conversion,and larval production.We tested daily substrate temperatures with dif-ferent population densities(i.e.,0,500,1000,5000,and 10000 larvae/pan),different pop-ulation sizes(i.e.,166,1000,and 10000 larvae at a fixed feed ratio)and air temperatures(i.e.,20 and 30℃)on various production parameters.Impacts of shifting larvae from 30 to 20℃on either day 9 or 11 were also determined.Larval activity increased substrate tem-peratures significantly(i.e.,at least 10℃above air temperatures).Low air temperature favored growth with the higher population sizes while high temperature favored growth with low population sizes.The greatest average individual larval weights(e.g.,0.126 and 0.124 g)and feed conversion ratios(e.g.,1.92 and 2.08 g/g)were recorded for either 10000 larvae reared at 20℃or 100 larvae reared at 30 C.Shifting temperatures from high(30℃)to low(20℃)in between(~10-d-old larvae)impacted larval production weights(16%increases)and feed conversion ratios(increased 14%).Facilities should consider the impact of larval density,population size,and air temperature during black soldier fly mass production as these factors impact overall larval production.展开更多
The black soldier fly(BSF),Hermetia illucens,is a promising insect for miti-gating solid waste problems as its larvae are able to bioconvert organic waste into valuable biomass.We recently reported a high-quality geno...The black soldier fly(BSF),Hermetia illucens,is a promising insect for miti-gating solid waste problems as its larvae are able to bioconvert organic waste into valuable biomass.We recently reported a high-quality genome assembly of the BSF;analysis of this genome sequence will further the understanding of insect biology and identify genes that can be manipulated to improve efficiency of bioconversion.To enable genetic manip-ulation of the BSF,we have established the first transgenic methods for this economically important insect.We cloned and identified the ubiquitous actin5C promoter(Hiactin5C-p3k)and 3 endogenous U6 promoters(HiU6:1,HiU6:2,and HiU6:3).The Hiactin5C pro-moter was used to drive expression of a hyperactive variant of the piggyBac transposase,which exhibited up to 6-fold improvement in transformation rate when compared to the wild-type transposase.Furthermore,we evaluated the 3 HiU6 promoters using this trans-genic system.HiU6:1 and HiU6:2 promoters provided the highest knockdown efficiency with RNAi and are thus promising candidates for future Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)development.Overall,our findings provide valuable genetic engineering toolkits for basic research and genetic manipulation of the BSF.展开更多
文摘Mass production of black soldier fly,Hermetia illucens(L.)(Diptera:Stra-tiomyidae),larvae results in massive heat generation,which impacts facility management,waste conversion,and larval production.We tested daily substrate temperatures with dif-ferent population densities(i.e.,0,500,1000,5000,and 10000 larvae/pan),different pop-ulation sizes(i.e.,166,1000,and 10000 larvae at a fixed feed ratio)and air temperatures(i.e.,20 and 30℃)on various production parameters.Impacts of shifting larvae from 30 to 20℃on either day 9 or 11 were also determined.Larval activity increased substrate tem-peratures significantly(i.e.,at least 10℃above air temperatures).Low air temperature favored growth with the higher population sizes while high temperature favored growth with low population sizes.The greatest average individual larval weights(e.g.,0.126 and 0.124 g)and feed conversion ratios(e.g.,1.92 and 2.08 g/g)were recorded for either 10000 larvae reared at 20℃or 100 larvae reared at 30 C.Shifting temperatures from high(30℃)to low(20℃)in between(~10-d-old larvae)impacted larval production weights(16%increases)and feed conversion ratios(increased 14%).Facilities should consider the impact of larval density,population size,and air temperature during black soldier fly mass production as these factors impact overall larval production.
基金supported by the National Natural Science Foundation of China(32021001,31830093)Strategic Priority Research Program of Chinese Academy of Sciences(Grant no.XDPB16).
文摘The black soldier fly(BSF),Hermetia illucens,is a promising insect for miti-gating solid waste problems as its larvae are able to bioconvert organic waste into valuable biomass.We recently reported a high-quality genome assembly of the BSF;analysis of this genome sequence will further the understanding of insect biology and identify genes that can be manipulated to improve efficiency of bioconversion.To enable genetic manip-ulation of the BSF,we have established the first transgenic methods for this economically important insect.We cloned and identified the ubiquitous actin5C promoter(Hiactin5C-p3k)and 3 endogenous U6 promoters(HiU6:1,HiU6:2,and HiU6:3).The Hiactin5C pro-moter was used to drive expression of a hyperactive variant of the piggyBac transposase,which exhibited up to 6-fold improvement in transformation rate when compared to the wild-type transposase.Furthermore,we evaluated the 3 HiU6 promoters using this trans-genic system.HiU6:1 and HiU6:2 promoters provided the highest knockdown efficiency with RNAi and are thus promising candidates for future Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)development.Overall,our findings provide valuable genetic engineering toolkits for basic research and genetic manipulation of the BSF.
