Objective Assessment of the bacterium L. sphaericus as a dual-action candidate for biological control of mosquito-borne diseases and bioremediation of toxic metals. Methods Larvae of the mosquito, C quinquefasciatus, ...Objective Assessment of the bacterium L. sphaericus as a dual-action candidate for biological control of mosquito-borne diseases and bioremediation of toxic metals. Methods Larvae of the mosquito, C quinquefasciatus, were first evaluated for metal tolerance and then exposed to 5 ppm cadmium, chromium, arsenic, and lead in assays together with seven strains of L. sphaericus. A probit regression analysis was used to estimate the LCso of Cd, Cr, As, and Pb to C. quinquefosciatus. An analysis of covariance and multifactorial ANOVA examined the metal biosorption and larvicidal properties of the seven strains of L. sphaericus. Results We found that L. sphaericus adsorbed the toxic metal ions and was toxic against mosquito larvae. The L. sphoericus strain 111（3）7 resulted in a larvae mortality of over 80% for all the tested metals. This strain also exhibited the capacity to adsorb 76% of arsenic, 32% of lead, 25% of chromium, and 7% of cadmium. Conclusion This study found combined metal adsorption and larval toxicity associated with three strains of L. sphaericus [111（3）7, OT4b.31, and CBAM5]. This suggests that a combination of these strains shows strong dual potential for biological control of mosquitos in heavy metal-contaminated areas and remediate the heavy metal contamination as well.展开更多
基金funded by the Research Fund from the Science Faculty of the Universidad de Los Andes and Microbiological Research Center(CIMIC)
文摘Objective Assessment of the bacterium L. sphaericus as a dual-action candidate for biological control of mosquito-borne diseases and bioremediation of toxic metals. Methods Larvae of the mosquito, C quinquefasciatus, were first evaluated for metal tolerance and then exposed to 5 ppm cadmium, chromium, arsenic, and lead in assays together with seven strains of L. sphaericus. A probit regression analysis was used to estimate the LCso of Cd, Cr, As, and Pb to C. quinquefosciatus. An analysis of covariance and multifactorial ANOVA examined the metal biosorption and larvicidal properties of the seven strains of L. sphaericus. Results We found that L. sphaericus adsorbed the toxic metal ions and was toxic against mosquito larvae. The L. sphoericus strain 111（3）7 resulted in a larvae mortality of over 80% for all the tested metals. This strain also exhibited the capacity to adsorb 76% of arsenic, 32% of lead, 25% of chromium, and 7% of cadmium. Conclusion This study found combined metal adsorption and larval toxicity associated with three strains of L. sphaericus [111（3）7, OT4b.31, and CBAM5]. This suggests that a combination of these strains shows strong dual potential for biological control of mosquitos in heavy metal-contaminated areas and remediate the heavy metal contamination as well.
