BACKGROUND The healthcare burden of inflammatory bowel disease(IBD)is rising globally and there are limited non-invasive biomarkers for accurate and early diagnosis.AIM To understand the important role that intestinal...BACKGROUND The healthcare burden of inflammatory bowel disease(IBD)is rising globally and there are limited non-invasive biomarkers for accurate and early diagnosis.AIM To understand the important role that intestinal microbiota play in IBD pathogenesis and identify anti-microbial antibody signatures that benefit clinical management of IBD patients.METHODS We performed serological profiling of 100 Crohn’s disease(CD)patients,100 ulcerative colitis(UC)patients and 100 healthy controls against 1173 bacterial and 397 viral proteins from 50 bacteria and 33 viruses on protein microarrays.The study subjects were randomly divided into discovery(n=150)and validation(n=150)sets.Statistical analysis was performed using R packages.RESULTS Anti-bacterial antibody responses showed greater differential prevalence among the three subject groups than anti-viral antibody responses.We identified novel antibodies against the antigens of Bacteroidetes vulgatus(BVU_0562)and Streptococcus pneumoniae(SP_1992)showing higher prevalence in CD patients relative to healthy controls.We also identified antibodies against the antigen of Streptococcus pyogenes(SPy_2009)showing higher prevalence in healthy controls relative to UC patients.Using these novel antibodies,we built biomarker panels with area under the curve(AUC)of 0.81,0.87,and 0.82 distinguishing CD vs control,UC vs control,and CD vs UC,respectively.Subgroup analysis revealed that penetrating CD behavior,colonic CD location,CD patients with a history of surgery,and extensive UC exhibited highest antibody prevalence among all patients.We demonstrated that autoantibodies and anti-microbial antibodies in CD patients had minimal correlation.CONCLUSION We have identified antibody signatures for CD and UC using a comprehensive analysis of antimicrobial antibody response in IBD.These antibodies and the source microorganisms of their target antigens improve our understanding of the role of specific microorganisms in IBD pathogenesis and,after future validation,should aid early and accurate diagnosis of IBD.展开更多
Contact stress is the important cause of wear and failure of knee joint prostheses implanted in the human body during movement.The calculation of contact stress on joint prostheses is often inaccurate due to the devia...Contact stress is the important cause of wear and failure of knee joint prostheses implanted in the human body during movement.The calculation of contact stress on joint prostheses is often inaccurate due to the deviation of joint position during activity.This paper proposes a correction method for contact stress based on finite element results.Firstly,the contact stress and contact radius between femoral and tibial prostheses of knee joint were calculated and analyzed by Hertz contact theory.Then,individualized finite element models based on the individualized geometry of the joint prosthesis and individualized loading were constructed,and the finite element contact stress was analyzed.The finite element results were calibrated utilizing the Hertz contact theory.Additionally,this study investigated the impact of three gait patterns(normal gait,medial thrust gait,and bouncing gait)on the contact stress experienced by the tibial insert.The research methods and results can provide ideas for theoretical analysis and finite element simulation of prosthetic joint contact,as well as offer theoretical support and analysis methods for evaluating the lifespan of prosthetic joints.展开更多
文摘BACKGROUND The healthcare burden of inflammatory bowel disease(IBD)is rising globally and there are limited non-invasive biomarkers for accurate and early diagnosis.AIM To understand the important role that intestinal microbiota play in IBD pathogenesis and identify anti-microbial antibody signatures that benefit clinical management of IBD patients.METHODS We performed serological profiling of 100 Crohn’s disease(CD)patients,100 ulcerative colitis(UC)patients and 100 healthy controls against 1173 bacterial and 397 viral proteins from 50 bacteria and 33 viruses on protein microarrays.The study subjects were randomly divided into discovery(n=150)and validation(n=150)sets.Statistical analysis was performed using R packages.RESULTS Anti-bacterial antibody responses showed greater differential prevalence among the three subject groups than anti-viral antibody responses.We identified novel antibodies against the antigens of Bacteroidetes vulgatus(BVU_0562)and Streptococcus pneumoniae(SP_1992)showing higher prevalence in CD patients relative to healthy controls.We also identified antibodies against the antigen of Streptococcus pyogenes(SPy_2009)showing higher prevalence in healthy controls relative to UC patients.Using these novel antibodies,we built biomarker panels with area under the curve(AUC)of 0.81,0.87,and 0.82 distinguishing CD vs control,UC vs control,and CD vs UC,respectively.Subgroup analysis revealed that penetrating CD behavior,colonic CD location,CD patients with a history of surgery,and extensive UC exhibited highest antibody prevalence among all patients.We demonstrated that autoantibodies and anti-microbial antibodies in CD patients had minimal correlation.CONCLUSION We have identified antibody signatures for CD and UC using a comprehensive analysis of antimicrobial antibody response in IBD.These antibodies and the source microorganisms of their target antigens improve our understanding of the role of specific microorganisms in IBD pathogenesis and,after future validation,should aid early and accurate diagnosis of IBD.
基金The National Natural Science Foundation of China(No.61602259,U1736204)Research Foundation for Advanced Talents of Nanjing University of Posts and Telecommunications(No.NY216022)the National Key Research and Development Program of China(No.2018YFC0830200).
基金supported by the National Natural Science Foundation of China(Grant numbers 12102287,12302477,11972243)the Fundamental Research Program of Shanxi Province(Grant number 202203021222081).
文摘Contact stress is the important cause of wear and failure of knee joint prostheses implanted in the human body during movement.The calculation of contact stress on joint prostheses is often inaccurate due to the deviation of joint position during activity.This paper proposes a correction method for contact stress based on finite element results.Firstly,the contact stress and contact radius between femoral and tibial prostheses of knee joint were calculated and analyzed by Hertz contact theory.Then,individualized finite element models based on the individualized geometry of the joint prosthesis and individualized loading were constructed,and the finite element contact stress was analyzed.The finite element results were calibrated utilizing the Hertz contact theory.Additionally,this study investigated the impact of three gait patterns(normal gait,medial thrust gait,and bouncing gait)on the contact stress experienced by the tibial insert.The research methods and results can provide ideas for theoretical analysis and finite element simulation of prosthetic joint contact,as well as offer theoretical support and analysis methods for evaluating the lifespan of prosthetic joints.
