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微孔板法与GeneXpert MTB/RIF技术检测结核分枝杆菌对利福平耐药性的价值 被引量:12
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作者 李静 梁亚萍 +3 位作者 王卓 陈基平 王智存 仵倩红 《中国防痨杂志》 CAS CSCD 2019年第2期162-168,共7页
目的探讨微孔板法和GeneXpert MTB/RIF(简称“GeneXpert”)技术检测MTB对利福平(RFP)耐药性的价值。方法收集陕西省结核病防治院2017年收住入院和门诊诊治的疑似结核病患者6893例,按照临床诊断标准确诊为结核病患者6086例,同时使用BACTE... 目的探讨微孔板法和GeneXpert MTB/RIF(简称“GeneXpert”)技术检测MTB对利福平(RFP)耐药性的价值。方法收集陕西省结核病防治院2017年收住入院和门诊诊治的疑似结核病患者6893例,按照临床诊断标准确诊为结核病患者6086例,同时使用BACTECMGIT960液体培养(简称“MGIT960法”)和GeneXpert技术检测上述确诊患者的425份临床标本;其中,痰标本217份、肺泡灌洗液标本170份、肺外标本(包括胸腹腔积液、脓液,脑脊液等)38份;排除3例报告错误或无法判读、2例培养结果污染的标本,有效标本份数为420份。对其中GeneXpert技术和MGIT 960液体培养均为阳性的372株临床分离株同时进行微孔板法、GeneXpert技术和MGIT 960耐药性检测,其中痰标本191例、肺泡灌洗液标本158例、肺外标本(包括胸腹腔积液、脓液及脑脊液等)23例,以MGIT960检测结果为标准,分析微孔板法和GeneXpert技术检测结核分枝杆菌对RFP耐药性的诊断价值。结果GeneXpert技术、微孔板法和MGIT960法对RFP耐药性检测总的阳性率分别为18.8%(70/372)、16.7%(62/372)、16.7%(62/372)。以MGIT960法检测结果为标准,微孔板法和GeneXpert法对利福平耐药性检测的敏感度、特异度、符合率、Kappa值分别为91.9%(57/62)、98.4%(305/310)、97.3%(362/372)、0.8,96.8%(60/62)、96.8%(300/310)、96.8%(360/372)、0.8;微孔板法和GeneXpert法对痰液、肺泡灌洗液、肺外标本利福平耐药性检测的敏感度、特异度、符合率、Kappa值分别为96.7%(29/30)、98.1%(158/161)、97.9%(187/191)、0.9,93.3%(28/30)、96.3%(155/161)、95.8%(183/191)、0.9,91.7%(22/24)、98.5%(132/134)、97.5%(154/158)、0.9和100.0%(24/24)、97.0%(130/134)、97.5%(154/158)、0.9,75.0%(6/8)、100.0%(15/15)、91.3%(21/23)、0.8,100.0%(8/8)、100.0%(15/15)、100.0%(23/23)、1.0。结论以MGIT960检测结果为标准,微孔板法和GeneXpert技术对利福平耐药性检测总的阳性率和不同标本对利福平耐药性检测的阳性率均具有较好的一致性。 展开更多
关键词 分枝杆菌 结核 利福平 结核 抗多种药物性 核酸扩增技术 微生物敏感性试验 对比研究
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Experimental study on Ti+Nb bearing ultra-low carbon bake hardening sheet steel hot-rolled in the ferrite region 被引量:6
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作者 ji-ping chen Yong-lin Kang +2 位作者 Ying-min Hao Guang-ming Liu Ai-ming Xiong 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2009年第5期540-548,共9页
A Ti+Nb bearing ultra-low carbon bake hardening sheet steel hot-rolled in the conventional austenite region and in the ferrite region with lubrication was experimentally studied. Subsequent cold rolling and continuou... A Ti+Nb bearing ultra-low carbon bake hardening sheet steel hot-rolled in the conventional austenite region and in the ferrite region with lubrication was experimentally studied. Subsequent cold rolling and continuous annealing processes were also conducted. The results show that microstructures of ultra-low carbon bake hardening hot strips at room temperature are basically irregular polygonal ferrites. The yield strength, ultimate tensile strength, n value, and r value of the No.2 specimen hot-rolled in the ferrite region with lubrication are 243 MPa, 364 MPa, 0.29, and 1.74, respectively, which are similar to those of the No.1 specimen hot-roiled in the conventional austenite region. The elongation rate and bake hardening value of No.2 specimen are 51% and 49.4 MPa, respectively, which are greater than those of No. 1 specimen. The No.2 specimen hot-rolled in the ferrite region with lubrication exhibits good mechanical properties and relatively excellent baking hardening performance. Therefore, the hot rolling experiment of Ti+Nb bearing ultra-low carbon bake hardening steel in the ferrite region with lubrication is feasible and can be considered in the future industrial trial production. 展开更多
关键词 ultra low carbon steel bake hardening TEXTURE MICROSTRUCTURE mechanical properties
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Effect of hydrogen-storage pressure on the detonation characteristics of emulsion explosives sensitized by glass microballoons 被引量:1
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作者 ji-ping chen Hong-hao Ma +2 位作者 Yi-xin Wang Liang-liang Huang Zhao-wu Shen 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2022年第5期747-754,共8页
In this study,hydrogen-storage glass microballoons were introduced into emulsion explosives to improve the detonation performance of the explosives.The effect of hydrogen-storage pressure on the detonation characteris... In this study,hydrogen-storage glass microballoons were introduced into emulsion explosives to improve the detonation performance of the explosives.The effect of hydrogen-storage pressure on the detonation characteristics of emulsion explosives was systematically investigated.Detonation velocity experiments shows that the change of sensitizing gas and the increase of hydrogen pressure have different effects on the detonation velocity.The experimental parameters of underwater explosion increase first and then decreases with the increase of hydrogen pressure.The decrease of these parameters indicates that the strength of glass microballoons is the limiting factor to improve the detonation performance of hydrogen-storage emulsion explosives.Compared with the traditional emulsion explosives,the maximum peak pressure of shock wave of hydrogen-storage emulsion explosives increases by 10.6%at 1.0 m and 10.2%at 1.2 m,the maximum values of shock impulse increase by 5.7%at 1.0 m and 19.4%at 1.2 m.The stored hydrogen has dual effects of sensitizers and energetic additives,which can improve the energy output of emulsion explosives. 展开更多
关键词 Emulsion explosives Hydrogen-storage pressure Glass microballoons Underwater explosion
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Interstitial Solution Carbon Concentration and Defects of Ti+Nb ULC-BH Steel by Internal Friction and Positron Annihilation Methods
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作者 ji-ping chen Yong-lin KANG 《Journal of Iron and Steel Research(International)》 SCIE EI CAS CSCD 2014年第1期91-97,共7页
The interstitial solution carbon concentration and defects in continuously annealed Ti+Nb bearing ULC- BH (ultra-low carbon bake hardening) steel samples are investigated by multi-functional internal friction appar... The interstitial solution carbon concentration and defects in continuously annealed Ti+Nb bearing ULC- BH (ultra-low carbon bake hardening) steel samples are investigated by multi-functional internal friction apparatus, positron annihilation lifetime spectroscopy (PALS) and coincidence Doppler broadening spectroscopy (CDBS). The relationship of internal friction peaks, interstitial solution carbon concentrations and movable dislocations in the sam- ples under different conditions is analyzed. A correlation of lifetime component v~ values with interstitial solution car- bon concentrations in the samples for different continuous annealing processes is established, while a correlation of li- fetime component r2 values with multi-vacancies, vacancy clusters, microvoids and other types of defects for various continuous annealing processes is also demonstrated. Furthermore, the average lifetime results illustrate the overall defect densities for various continuous annealing processes. The CDBS analysis reflects the chemical surroundings of the defects at the annihilation sites and reveals that the peak heights of the ratio curves relate to the total number of defects such as interstitial carbon atoms, dislocations, vacancies and other types of defects. The results show that in- ternal friction, PALS and CDBS are effective techniques to identify and characterize the interstitial solution carbon concentration, multi-vacancies, vacancy clusters, microvoids and other types of microscopic defects in annealed Ti+ Nb bearing ULC-BH steel. 展开更多
关键词 ULC-BH steels internal frictions positron annihilation lifetimes coincidence Doppler broadening
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