Cancer is a big challenge that has plagued the human beings for ages and one of the most effective treatments is chemotherapy. However, the low tumor-targeting ability limits the wide clinical application of chemother...Cancer is a big challenge that has plagued the human beings for ages and one of the most effective treatments is chemotherapy. However, the low tumor-targeting ability limits the wide clinical application of chemotherapy. The microenvironment plays a critical role in many aspects of tumor genesis. It generates the tumor vasculature and it is highly implicated in the progression to metastasis. To maintain a suitable environment for tumor progression, there are special microenvironment in tumor cell, such as low pH, high level of glutathione(GSH) and reactive oxygen species(ROS), and more special enzymes, which is different to normal cell. Microenvironment-targeted therapy strategy could create new opportunities for therapeutic targeting. Compared to other targeting strategies, microenvironment-targeted therapy strategy will control the drug release into tumor cells more accurately. Redox responsive drug delivery systems(DDSs) are developed based on the high level of GSH in tumor cells. However, there are also GSH in normal cell though its level is lower. In order to control the release of drugs more accurately and reduce side effects, other drug release stimuli have been introduced to redox responsive DDSs. Under the synergistic reaction of two stimuli, redox dual-stimuli responsive DDSs will control the release of drugs more accurately and quickly and even increase the accumulation. This review summarizes strategies of redox dual-stimuli responsive DDSs such as pH, light, enzyme, ROS, and magnetic guide to delivery chemotherapeutic agents more accurately, aiming at providing new ideas for further promoting the drug release,enhancing tumor-targeting and improving anticancer effects. To better illustrate the redox dual-stimuli responsive DDS, preparations of carriers are also briefly described in the review.展开更多
AIM: To study the effect of glucose on sodium butyrate- induced proliferation inhibition and apoptosis in HT-29 cell line, and explored its possible mechanisms. METHODS: HT-29 cells were grown in RPMI-1640 medium supp...AIM: To study the effect of glucose on sodium butyrate- induced proliferation inhibition and apoptosis in HT-29 cell line, and explored its possible mechanisms. METHODS: HT-29 cells were grown in RPMI-1640 medium supplemented with 10% fetal calf serum, and were allowed to adhere for 24 h, and then replaced with experimental medium. Cell survival rates were detected by MTT assay. Apoptosis was detected by TUNEL assay. Glucose transport protein 1 (GLUT1) and monocarboxylate transporter 1 (MCT1) mRNA expression was detected by RT-PCR. RESULTS: Low concentration of glucose induced apoptosis and regulated proliferation in HT-29 cell line, and glucose can obviously inhibit the effect of proliferation inhibition and apoptosis induced by sodium butyrate. Glucose also down-regulated the expression of MCT1mRNA (0.28 ± 0.07 vs 0.19 ± 0.10, P < 0.05), and decreased the expression of GLUT1mRNA slightly (0.18 ± 0.04 vs 0.13 ± 0.03, P < 0.05). CONCLUSION: Glucose can regulate the effect of proliferation inhibition and apoptosis induced by sodium butyrate and this influence may be associated with the intracellular concentration of glucose and sodium butyrate.展开更多
The lack of effective methods of diagnosis and treatment presents a major barrier to combat against tumor.The biomimetic concept is an emerging field that expresses great application potential in tumor fighting.Strate...The lack of effective methods of diagnosis and treatment presents a major barrier to combat against tumor.The biomimetic concept is an emerging field that expresses great application potential in tumor fighting.Strategy for combining nano-systems with biomimetic technology has gained increasing attention that is proved bioinspired,environmentally benign,and promising.Herein,we provide an up-to-date review of biomimetic nanosystems as well as their applications in tumor therapy.In addition,the challenges and future directions of biomimetic nano-systems to achieve clinical translation are also pointed out.展开更多
Public bulletin boards are widely used in various public places,but due to the large size of the billboards,it is not easy to carry,and the traditional billboards are integrated,when the billboards need to be transfer...Public bulletin boards are widely used in various public places,but due to the large size of the billboards,it is not easy to carry,and the traditional billboards are integrated,when the billboards need to be transferred,the limited storage space of the vehicle needs to be occupied It is extremely inconvenient,and at night,the traditional bulletin boards need to install additional light sources to illuminate them,which greatly increases energy consumption.Based on this paper designed a new structure of the bulletin board,this structure can make the bulletin board when not in use completely folded up and easy to carry,and in use can be completely unfolded and in the bottom of the added retractable support leg structure can be placed anywhere,and according to the terrain or human will to adjust the height.A solar panel is placed on the top of the bulletin board,which can store solar energy during the day and supply energy to the LED lights on the top at night for lighting,which saves energy and is very environmentally friendly.展开更多
锂硫电池固有的缓慢转化动力学和严重的穿梭效应导致其可逆容量和循环寿命差,严重阻碍了其实际应用.为了解决这些问题,我们设计并构建了一种Ni/Ni_(2)P异质结嵌入介孔碳纳米球的复合材料(Ni/Ni_(2)P-MCN),将其用于锂硫电池隔膜改性以促...锂硫电池固有的缓慢转化动力学和严重的穿梭效应导致其可逆容量和循环寿命差,严重阻碍了其实际应用.为了解决这些问题,我们设计并构建了一种Ni/Ni_(2)P异质结嵌入介孔碳纳米球的复合材料(Ni/Ni_(2)P-MCN),将其用于锂硫电池隔膜改性以促进多硫化物的催化转化.研究发现,Ni/Ni_(2)P-MCN改性隔膜可以通过丰富的异质结化学吸附位点吸附多硫化物、抑制穿梭效应,而且对多硫化物的转化具有优异的催化活性.此外,具有暴露介孔结构的导电碳球可以作为物理屏障,容纳沉积的不溶性Li_(2)S.因此,使用Ni/Ni_(2)P-MCN改性隔膜的电池显示出优异的倍率性能(5 C下431 mA h g^(-1))和循环稳定性(1500次循环下平均容量衰减约0.031%).在4.2 mg cm^(-2)的高载硫下,输出面积比容量约3.5 mA h cm^(-2).我们认为,这种独特的Ni/Ni_(2)P异质结/多孔碳复合材料在高性能、可持续储能器件中具备巨大的应用潜力.展开更多
Triptolide is a diterpenoid compound that inhibits the inflammation of rheumatoid arthritis(RA).However,the use of triptolide is limited due to its strong gastrointestinal toxicity.The purpose of this work was to deve...Triptolide is a diterpenoid compound that inhibits the inflammation of rheumatoid arthritis(RA).However,the use of triptolide is limited due to its strong gastrointestinal toxicity.The purpose of this work was to develop a transdermal delivery system for triptolide to reduce this toxicity.A microemulsion-based hydrogel(MBH)was prepared from the combination of Gemseal 40-oleic acid as oil phase,Tween 80-labrasol as surfactant,anhydrous ethanol as co-surfactant,water as aqueous phase and Poloxamer 407 as hydrogel matrix.Rheological measurements,environmental scanning electron microscopy(ESEM)and transdermal experiments in vitro were used to characterize triptolide-loaded and blank MBH preparations.The effects of Poloxamer 407 and triptolide on the rheological properties and microstructures of the MBH were determined.Transparent and homogeneous MBH could only be formed when the concentration of Poloxamer 407 in the selected O/W microemulsion was in the range of 14.0–16.0%(w/w).When the concentration of Poloxamer 407 increased,the rheological properties such as the yield stresses(sy),storage and loss moduli(G′,G″)of the formulations increased,and the network structures became more compact.The addition of triptolide did not change the interconnected network structures of the MBH preparations.MBH preparations afford a better sustained release profile when compared to microemulsions,a finding confirmed by an in vitro permeation test in mice.MBH appears to be a promising vehicle for transdermal delivery of triptolide.展开更多
基金National Natural Science Foundation of China (81202480,81302723)Natural Science Foundation of Liaoning Province (2015020749)。
文摘Cancer is a big challenge that has plagued the human beings for ages and one of the most effective treatments is chemotherapy. However, the low tumor-targeting ability limits the wide clinical application of chemotherapy. The microenvironment plays a critical role in many aspects of tumor genesis. It generates the tumor vasculature and it is highly implicated in the progression to metastasis. To maintain a suitable environment for tumor progression, there are special microenvironment in tumor cell, such as low pH, high level of glutathione(GSH) and reactive oxygen species(ROS), and more special enzymes, which is different to normal cell. Microenvironment-targeted therapy strategy could create new opportunities for therapeutic targeting. Compared to other targeting strategies, microenvironment-targeted therapy strategy will control the drug release into tumor cells more accurately. Redox responsive drug delivery systems(DDSs) are developed based on the high level of GSH in tumor cells. However, there are also GSH in normal cell though its level is lower. In order to control the release of drugs more accurately and reduce side effects, other drug release stimuli have been introduced to redox responsive DDSs. Under the synergistic reaction of two stimuli, redox dual-stimuli responsive DDSs will control the release of drugs more accurately and quickly and even increase the accumulation. This review summarizes strategies of redox dual-stimuli responsive DDSs such as pH, light, enzyme, ROS, and magnetic guide to delivery chemotherapeutic agents more accurately, aiming at providing new ideas for further promoting the drug release,enhancing tumor-targeting and improving anticancer effects. To better illustrate the redox dual-stimuli responsive DDS, preparations of carriers are also briefly described in the review.
基金Supported by the Key Technologies R&D Program of Hubei Province, No. 2004AA304B08
文摘AIM: To study the effect of glucose on sodium butyrate- induced proliferation inhibition and apoptosis in HT-29 cell line, and explored its possible mechanisms. METHODS: HT-29 cells were grown in RPMI-1640 medium supplemented with 10% fetal calf serum, and were allowed to adhere for 24 h, and then replaced with experimental medium. Cell survival rates were detected by MTT assay. Apoptosis was detected by TUNEL assay. Glucose transport protein 1 (GLUT1) and monocarboxylate transporter 1 (MCT1) mRNA expression was detected by RT-PCR. RESULTS: Low concentration of glucose induced apoptosis and regulated proliferation in HT-29 cell line, and glucose can obviously inhibit the effect of proliferation inhibition and apoptosis induced by sodium butyrate. Glucose also down-regulated the expression of MCT1mRNA (0.28 ± 0.07 vs 0.19 ± 0.10, P < 0.05), and decreased the expression of GLUT1mRNA slightly (0.18 ± 0.04 vs 0.13 ± 0.03, P < 0.05). CONCLUSION: Glucose can regulate the effect of proliferation inhibition and apoptosis induced by sodium butyrate and this influence may be associated with the intracellular concentration of glucose and sodium butyrate.
基金We are grateful to financial support from National Natural Science Foundation of China(81202480,81302723)Natural Science Foundation of Liaoning Province(2015020749).
文摘The lack of effective methods of diagnosis and treatment presents a major barrier to combat against tumor.The biomimetic concept is an emerging field that expresses great application potential in tumor fighting.Strategy for combining nano-systems with biomimetic technology has gained increasing attention that is proved bioinspired,environmentally benign,and promising.Herein,we provide an up-to-date review of biomimetic nanosystems as well as their applications in tumor therapy.In addition,the challenges and future directions of biomimetic nano-systems to achieve clinical translation are also pointed out.
基金College Students Innovation and Entrepreneurship Project(S202214435012).
文摘Public bulletin boards are widely used in various public places,but due to the large size of the billboards,it is not easy to carry,and the traditional billboards are integrated,when the billboards need to be transferred,the limited storage space of the vehicle needs to be occupied It is extremely inconvenient,and at night,the traditional bulletin boards need to install additional light sources to illuminate them,which greatly increases energy consumption.Based on this paper designed a new structure of the bulletin board,this structure can make the bulletin board when not in use completely folded up and easy to carry,and in use can be completely unfolded and in the bottom of the added retractable support leg structure can be placed anywhere,and according to the terrain or human will to adjust the height.A solar panel is placed on the top of the bulletin board,which can store solar energy during the day and supply energy to the LED lights on the top at night for lighting,which saves energy and is very environmentally friendly.
基金financially supported by the National Natural Science Foundation of China(52072124)Shanghai Municipal Science and Technology Major Project(2018SHZDZX03)+3 种基金the Natural Science Foundation of Shanghai(20ZR1414900)the Leading Talents in Shanghai in2018the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learningthe 111 Project(B14018)。
文摘锂硫电池固有的缓慢转化动力学和严重的穿梭效应导致其可逆容量和循环寿命差,严重阻碍了其实际应用.为了解决这些问题,我们设计并构建了一种Ni/Ni_(2)P异质结嵌入介孔碳纳米球的复合材料(Ni/Ni_(2)P-MCN),将其用于锂硫电池隔膜改性以促进多硫化物的催化转化.研究发现,Ni/Ni_(2)P-MCN改性隔膜可以通过丰富的异质结化学吸附位点吸附多硫化物、抑制穿梭效应,而且对多硫化物的转化具有优异的催化活性.此外,具有暴露介孔结构的导电碳球可以作为物理屏障,容纳沉积的不溶性Li_(2)S.因此,使用Ni/Ni_(2)P-MCN改性隔膜的电池显示出优异的倍率性能(5 C下431 mA h g^(-1))和循环稳定性(1500次循环下平均容量衰减约0.031%).在4.2 mg cm^(-2)的高载硫下,输出面积比容量约3.5 mA h cm^(-2).我们认为,这种独特的Ni/Ni_(2)P异质结/多孔碳复合材料在高性能、可持续储能器件中具备巨大的应用潜力.
基金by the Advanced Technology Platform of TCM Delivery System from State Center for Drug Research and Development(No.2009ZX09310-005)Jiangxi Young Scientist Training Program(No.20112BCB23011)Jiangxi Science and Technology Pillar Program(No.2010BSA18400).
文摘Triptolide is a diterpenoid compound that inhibits the inflammation of rheumatoid arthritis(RA).However,the use of triptolide is limited due to its strong gastrointestinal toxicity.The purpose of this work was to develop a transdermal delivery system for triptolide to reduce this toxicity.A microemulsion-based hydrogel(MBH)was prepared from the combination of Gemseal 40-oleic acid as oil phase,Tween 80-labrasol as surfactant,anhydrous ethanol as co-surfactant,water as aqueous phase and Poloxamer 407 as hydrogel matrix.Rheological measurements,environmental scanning electron microscopy(ESEM)and transdermal experiments in vitro were used to characterize triptolide-loaded and blank MBH preparations.The effects of Poloxamer 407 and triptolide on the rheological properties and microstructures of the MBH were determined.Transparent and homogeneous MBH could only be formed when the concentration of Poloxamer 407 in the selected O/W microemulsion was in the range of 14.0–16.0%(w/w).When the concentration of Poloxamer 407 increased,the rheological properties such as the yield stresses(sy),storage and loss moduli(G′,G″)of the formulations increased,and the network structures became more compact.The addition of triptolide did not change the interconnected network structures of the MBH preparations.MBH preparations afford a better sustained release profile when compared to microemulsions,a finding confirmed by an in vitro permeation test in mice.MBH appears to be a promising vehicle for transdermal delivery of triptolide.
